[2023-03-18 00:21:54,212] [INFO] DFAST_QC pipeline started.
[2023-03-18 00:21:54,212] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 00:21:54,212] [INFO] DQC Reference Directory: /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference
[2023-03-18 00:21:55,650] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 00:21:55,650] [INFO] Task started: Prodigal
[2023-03-18 00:21:55,651] [INFO] Running command: cat /var/lib/cwl/stg2bae2deb-203b-4085-b256-326f0bd0a7ed/OceanDNA-b44723.fa | prodigal -d OceanDNA-b44723/cds.fna -a OceanDNA-b44723/protein.faa -g 11 -q > /dev/null
[2023-03-18 00:22:04,068] [INFO] Task succeeded: Prodigal
[2023-03-18 00:22:04,068] [INFO] Task started: HMMsearch
[2023-03-18 00:22:04,068] [INFO] Running command: hmmsearch --tblout OceanDNA-b44723/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/reference_markers.hmm OceanDNA-b44723/protein.faa > /dev/null
[2023-03-18 00:22:04,225] [INFO] Task succeeded: HMMsearch
[2023-03-18 00:22:04,225] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg2bae2deb-203b-4085-b256-326f0bd0a7ed/OceanDNA-b44723.fa]
[2023-03-18 00:22:04,237] [INFO] Query marker FASTA was written to OceanDNA-b44723/markers.fasta
[2023-03-18 00:22:04,238] [INFO] Task started: Blastn
[2023-03-18 00:22:04,238] [INFO] Running command: blastn -query OceanDNA-b44723/markers.fasta -db /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/reference_markers.fasta -out OceanDNA-b44723/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 00:22:04,736] [INFO] Task succeeded: Blastn
[2023-03-18 00:22:04,736] [INFO] Selected 10 target genomes.
[2023-03-18 00:22:04,737] [INFO] Target genome list was writen to OceanDNA-b44723/target_genomes.txt
[2023-03-18 00:22:04,744] [INFO] Task started: fastANI
[2023-03-18 00:22:04,744] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bae2deb-203b-4085-b256-326f0bd0a7ed/OceanDNA-b44723.fa --refList OceanDNA-b44723/target_genomes.txt --output OceanDNA-b44723/fastani_result.tsv --threads 1
[2023-03-18 00:22:09,787] [INFO] Task succeeded: fastANI
[2023-03-18 00:22:09,787] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 00:22:09,788] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 00:22:09,788] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 00:22:09,788] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 00:22:09,788] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 00:22:09,788] [INFO] DFAST Taxonomy check result was written to OceanDNA-b44723/tc_result.tsv
[2023-03-18 00:22:09,788] [INFO] ===== Taxonomy check completed =====
[2023-03-18 00:22:09,788] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 00:22:09,789] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/checkm_data
[2023-03-18 00:22:09,791] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 00:22:09,795] [INFO] Task started: CheckM
[2023-03-18 00:22:09,795] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b44723/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b44723/checkm_input OceanDNA-b44723/checkm_result
[2023-03-18 00:22:37,113] [INFO] Task succeeded: CheckM
[2023-03-18 00:22:37,113] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 54.77%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 00:22:37,115] [INFO] ===== Completeness check finished =====
[2023-03-18 00:22:37,115] [INFO] ===== Start GTDB Search =====
[2023-03-18 00:22:37,115] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b44723/markers.fasta)
[2023-03-18 00:22:37,117] [INFO] Task started: Blastn
[2023-03-18 00:22:37,117] [INFO] Running command: blastn -query OceanDNA-b44723/markers.fasta -db /var/lib/cwl/stg865eb1b4-c9a0-469e-9c08-9035788ff8e2/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b44723/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 00:22:37,774] [INFO] Task succeeded: Blastn
[2023-03-18 00:22:37,775] [INFO] Selected 11 target genomes.
[2023-03-18 00:22:37,775] [INFO] Target genome list was writen to OceanDNA-b44723/target_genomes_gtdb.txt
[2023-03-18 00:22:37,790] [INFO] Task started: fastANI
[2023-03-18 00:22:37,790] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bae2deb-203b-4085-b256-326f0bd0a7ed/OceanDNA-b44723.fa --refList OceanDNA-b44723/target_genomes_gtdb.txt --output OceanDNA-b44723/fastani_result_gtdb.tsv --threads 1
[2023-03-18 00:22:43,001] [INFO] Task succeeded: fastANI
[2023-03-18 00:22:43,005] [INFO] Found 4 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 00:22:43,005] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902562015.1	s__Arctic96AD-7 sp902562015	82.6108	178	393	d__Bacteria;p__SAR324;c__SAR324;o__SAR324;f__NAC60-12;g__Arctic96AD-7	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902564345.1	s__Arctic96AD-7 sp902564345	80.7885	189	393	d__Bacteria;p__SAR324;c__SAR324;o__SAR324;f__NAC60-12;g__Arctic96AD-7	95.0	98.84	98.84	0.77	0.77	2	-
GCA_002716165.1	s__Arctic96AD-7 sp002716165	80.514	179	393	d__Bacteria;p__SAR324;c__SAR324;o__SAR324;f__NAC60-12;g__Arctic96AD-7	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001627865.1	s__Arctic96AD-7 sp001627865	77.2335	169	393	d__Bacteria;p__SAR324;c__SAR324;o__SAR324;f__NAC60-12;g__Arctic96AD-7	95.0	99.71	99.45	0.95	0.94	9	-
--------------------------------------------------------------------------------
[2023-03-18 00:22:43,005] [INFO] GTDB search result was written to OceanDNA-b44723/result_gtdb.tsv
[2023-03-18 00:22:43,005] [INFO] ===== GTDB Search completed =====
[2023-03-18 00:22:43,006] [INFO] DFAST_QC result json was written to OceanDNA-b44723/dqc_result.json
[2023-03-18 00:22:43,006] [INFO] DFAST_QC completed!
[2023-03-18 00:22:43,006] [INFO] Total running time: 0h0m49s