[2023-03-15 06:03:50,991] [INFO] DFAST_QC pipeline started.
[2023-03-15 06:03:50,991] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 06:03:50,991] [INFO] DQC Reference Directory: /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference
[2023-03-15 06:03:52,084] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 06:03:52,084] [INFO] Task started: Prodigal
[2023-03-15 06:03:52,084] [INFO] Running command: cat /var/lib/cwl/stg8027bbd0-37e9-4ef8-a7f9-278c43233780/OceanDNA-b45236.fa | prodigal -d OceanDNA-b45236/cds.fna -a OceanDNA-b45236/protein.faa -g 11 -q > /dev/null
[2023-03-15 06:04:18,078] [INFO] Task succeeded: Prodigal
[2023-03-15 06:04:18,079] [INFO] Task started: HMMsearch
[2023-03-15 06:04:18,079] [INFO] Running command: hmmsearch --tblout OceanDNA-b45236/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/reference_markers.hmm OceanDNA-b45236/protein.faa > /dev/null
[2023-03-15 06:04:18,287] [INFO] Task succeeded: HMMsearch
[2023-03-15 06:04:18,288] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg8027bbd0-37e9-4ef8-a7f9-278c43233780/OceanDNA-b45236.fa]
[2023-03-15 06:04:18,311] [INFO] Query marker FASTA was written to OceanDNA-b45236/markers.fasta
[2023-03-15 06:04:18,313] [INFO] Task started: Blastn
[2023-03-15 06:04:18,313] [INFO] Running command: blastn -query OceanDNA-b45236/markers.fasta -db /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/reference_markers.fasta -out OceanDNA-b45236/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 06:04:18,853] [INFO] Task succeeded: Blastn
[2023-03-15 06:04:18,853] [INFO] Selected 10 target genomes.
[2023-03-15 06:04:18,854] [INFO] Target genome list was writen to OceanDNA-b45236/target_genomes.txt
[2023-03-15 06:04:18,859] [INFO] Task started: fastANI
[2023-03-15 06:04:18,859] [INFO] Running command: fastANI --query /var/lib/cwl/stg8027bbd0-37e9-4ef8-a7f9-278c43233780/OceanDNA-b45236.fa --refList OceanDNA-b45236/target_genomes.txt --output OceanDNA-b45236/fastani_result.tsv --threads 1
[2023-03-15 06:04:25,521] [INFO] Task succeeded: fastANI
[2023-03-15 06:04:25,522] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 06:04:25,522] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 06:04:25,522] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 06:04:25,522] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 06:04:25,522] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 06:04:25,522] [INFO] DFAST Taxonomy check result was written to OceanDNA-b45236/tc_result.tsv
[2023-03-15 06:04:25,522] [INFO] ===== Taxonomy check completed =====
[2023-03-15 06:04:25,522] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 06:04:25,523] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/checkm_data
[2023-03-15 06:04:25,526] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 06:04:25,532] [INFO] Task started: CheckM
[2023-03-15 06:04:25,532] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b45236/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b45236/checkm_input OceanDNA-b45236/checkm_result
[2023-03-15 06:05:30,438] [INFO] Task succeeded: CheckM
[2023-03-15 06:05:30,439] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 4.69%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-15 06:05:30,468] [INFO] ===== Completeness check finished =====
[2023-03-15 06:05:30,468] [INFO] ===== Start GTDB Search =====
[2023-03-15 06:05:30,469] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b45236/markers.fasta)
[2023-03-15 06:05:30,470] [INFO] Task started: Blastn
[2023-03-15 06:05:30,470] [INFO] Running command: blastn -query OceanDNA-b45236/markers.fasta -db /var/lib/cwl/stg815fc5a8-a8c4-4d3f-82e6-48bd35a405e0/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b45236/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 06:05:31,305] [INFO] Task succeeded: Blastn
[2023-03-15 06:05:31,309] [INFO] Selected 13 target genomes.
[2023-03-15 06:05:31,310] [INFO] Target genome list was writen to OceanDNA-b45236/target_genomes_gtdb.txt
[2023-03-15 06:05:31,326] [INFO] Task started: fastANI
[2023-03-15 06:05:31,326] [INFO] Running command: fastANI --query /var/lib/cwl/stg8027bbd0-37e9-4ef8-a7f9-278c43233780/OceanDNA-b45236.fa --refList OceanDNA-b45236/target_genomes_gtdb.txt --output OceanDNA-b45236/fastani_result_gtdb.tsv --threads 1
[2023-03-15 06:05:39,228] [INFO] Task succeeded: fastANI
[2023-03-15 06:05:39,231] [INFO] Found 3 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 06:05:39,232] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002729885.1	s__UBA3465 sp002729885	99.9829	1437	1453	d__Bacteria;p__Spirochaetota;c__Leptospirae;o__Leptospirales;f__Leptonemataceae;g__UBA3465	95.0	99.96	99.92	0.99	0.98	4	conclusive
GCA_002717505.1	s__UBA3465 sp002717505	81.3688	780	1453	d__Bacteria;p__Spirochaetota;c__Leptospirae;o__Leptospirales;f__Leptonemataceae;g__UBA3465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002730875.1	s__UBA3465 sp002730875	78.789	515	1453	d__Bacteria;p__Spirochaetota;c__Leptospirae;o__Leptospirales;f__Leptonemataceae;g__UBA3465	95.0	99.98	99.97	0.99	0.98	7	-
--------------------------------------------------------------------------------
[2023-03-15 06:05:39,233] [INFO] GTDB search result was written to OceanDNA-b45236/result_gtdb.tsv
[2023-03-15 06:05:39,235] [INFO] ===== GTDB Search completed =====
[2023-03-15 06:05:39,238] [INFO] DFAST_QC result json was written to OceanDNA-b45236/dqc_result.json
[2023-03-15 06:05:39,238] [INFO] DFAST_QC completed!
[2023-03-15 06:05:39,238] [INFO] Total running time: 0h1m48s