[2023-03-15 03:02:04,956] [INFO] DFAST_QC pipeline started.
[2023-03-15 03:02:04,957] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 03:02:04,957] [INFO] DQC Reference Directory: /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference
[2023-03-15 03:02:06,581] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 03:02:06,582] [INFO] Task started: Prodigal
[2023-03-15 03:02:06,582] [INFO] Running command: cat /var/lib/cwl/stgff31f0c8-21ee-4331-ace6-d34e6b351700/OceanDNA-b45438.fa | prodigal -d OceanDNA-b45438/cds.fna -a OceanDNA-b45438/protein.faa -g 11 -q > /dev/null
[2023-03-15 03:02:31,849] [INFO] Task succeeded: Prodigal
[2023-03-15 03:02:31,849] [INFO] Task started: HMMsearch
[2023-03-15 03:02:31,849] [INFO] Running command: hmmsearch --tblout OceanDNA-b45438/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/reference_markers.hmm OceanDNA-b45438/protein.faa > /dev/null
[2023-03-15 03:02:32,040] [INFO] Task succeeded: HMMsearch
[2023-03-15 03:02:32,040] [INFO] Found 6/6 markers.
[2023-03-15 03:02:32,063] [INFO] Query marker FASTA was written to OceanDNA-b45438/markers.fasta
[2023-03-15 03:02:32,064] [INFO] Task started: Blastn
[2023-03-15 03:02:32,064] [INFO] Running command: blastn -query OceanDNA-b45438/markers.fasta -db /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/reference_markers.fasta -out OceanDNA-b45438/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:02:32,579] [INFO] Task succeeded: Blastn
[2023-03-15 03:02:32,580] [INFO] Selected 15 target genomes.
[2023-03-15 03:02:32,580] [INFO] Target genome list was writen to OceanDNA-b45438/target_genomes.txt
[2023-03-15 03:02:32,591] [INFO] Task started: fastANI
[2023-03-15 03:02:32,591] [INFO] Running command: fastANI --query /var/lib/cwl/stgff31f0c8-21ee-4331-ace6-d34e6b351700/OceanDNA-b45438.fa --refList OceanDNA-b45438/target_genomes.txt --output OceanDNA-b45438/fastani_result.tsv --threads 1
[2023-03-15 03:02:41,706] [INFO] Task succeeded: fastANI
[2023-03-15 03:02:41,706] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 03:02:41,707] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 03:02:41,707] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 03:02:41,707] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 03:02:41,707] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 03:02:41,707] [INFO] DFAST Taxonomy check result was written to OceanDNA-b45438/tc_result.tsv
[2023-03-15 03:02:41,708] [INFO] ===== Taxonomy check completed =====
[2023-03-15 03:02:41,708] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 03:02:41,708] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/checkm_data
[2023-03-15 03:02:41,711] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 03:02:41,720] [INFO] Task started: CheckM
[2023-03-15 03:02:41,720] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b45438/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b45438/checkm_input OceanDNA-b45438/checkm_result
[2023-03-15 03:03:47,914] [INFO] Task succeeded: CheckM
[2023-03-15 03:03:47,915] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 94.91%
Contamintation: 6.94%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 03:03:47,921] [INFO] ===== Completeness check finished =====
[2023-03-15 03:03:47,921] [INFO] ===== Start GTDB Search =====
[2023-03-15 03:03:47,921] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b45438/markers.fasta)
[2023-03-15 03:03:47,922] [INFO] Task started: Blastn
[2023-03-15 03:03:47,922] [INFO] Running command: blastn -query OceanDNA-b45438/markers.fasta -db /var/lib/cwl/stg3f8ded07-be51-4e98-9513-97ba5402744d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b45438/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:03:48,682] [INFO] Task succeeded: Blastn
[2023-03-15 03:03:48,684] [INFO] Selected 27 target genomes.
[2023-03-15 03:03:48,684] [INFO] Target genome list was writen to OceanDNA-b45438/target_genomes_gtdb.txt
[2023-03-15 03:03:48,706] [INFO] Task started: fastANI
[2023-03-15 03:03:48,707] [INFO] Running command: fastANI --query /var/lib/cwl/stgff31f0c8-21ee-4331-ace6-d34e6b351700/OceanDNA-b45438.fa --refList OceanDNA-b45438/target_genomes_gtdb.txt --output OceanDNA-b45438/fastani_result_gtdb.tsv --threads 1
[2023-03-15 03:04:02,124] [INFO] Task succeeded: fastANI
[2023-03-15 03:04:02,126] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 03:04:02,127] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018668635.1	s__JABJCN01 sp018668635	78.8641	114	768	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Opitutales;f__JABJCN01;g__JABJCN01	95.0	99.52	99.29	0.87	0.84	6	-
--------------------------------------------------------------------------------
[2023-03-15 03:04:02,128] [INFO] GTDB search result was written to OceanDNA-b45438/result_gtdb.tsv
[2023-03-15 03:04:02,129] [INFO] ===== GTDB Search completed =====
[2023-03-15 03:04:02,130] [INFO] DFAST_QC result json was written to OceanDNA-b45438/dqc_result.json
[2023-03-15 03:04:02,130] [INFO] DFAST_QC completed!
[2023-03-15 03:04:02,130] [INFO] Total running time: 0h1m57s