[2023-03-15 04:45:50,350] [INFO] DFAST_QC pipeline started.
[2023-03-15 04:45:50,352] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 04:45:50,352] [INFO] DQC Reference Directory: /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference
[2023-03-15 04:45:51,439] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 04:45:51,440] [INFO] Task started: Prodigal
[2023-03-15 04:45:51,440] [INFO] Running command: cat /var/lib/cwl/stga9c1db63-0e1b-42cd-be0a-c40ad894ed99/OceanDNA-b45445.fa | prodigal -d OceanDNA-b45445/cds.fna -a OceanDNA-b45445/protein.faa -g 11 -q > /dev/null
[2023-03-15 04:46:30,987] [INFO] Task succeeded: Prodigal
[2023-03-15 04:46:30,987] [INFO] Task started: HMMsearch
[2023-03-15 04:46:30,988] [INFO] Running command: hmmsearch --tblout OceanDNA-b45445/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/reference_markers.hmm OceanDNA-b45445/protein.faa > /dev/null
[2023-03-15 04:46:31,384] [INFO] Task succeeded: HMMsearch
[2023-03-15 04:46:31,384] [INFO] Found 6/6 markers.
[2023-03-15 04:46:31,435] [INFO] Query marker FASTA was written to OceanDNA-b45445/markers.fasta
[2023-03-15 04:46:31,436] [INFO] Task started: Blastn
[2023-03-15 04:46:31,437] [INFO] Running command: blastn -query OceanDNA-b45445/markers.fasta -db /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/reference_markers.fasta -out OceanDNA-b45445/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 04:46:32,068] [INFO] Task succeeded: Blastn
[2023-03-15 04:46:32,081] [INFO] Selected 15 target genomes.
[2023-03-15 04:46:32,081] [INFO] Target genome list was writen to OceanDNA-b45445/target_genomes.txt
[2023-03-15 04:46:32,091] [INFO] Task started: fastANI
[2023-03-15 04:46:32,091] [INFO] Running command: fastANI --query /var/lib/cwl/stga9c1db63-0e1b-42cd-be0a-c40ad894ed99/OceanDNA-b45445.fa --refList OceanDNA-b45445/target_genomes.txt --output OceanDNA-b45445/fastani_result.tsv --threads 1
[2023-03-15 04:46:40,030] [INFO] Task succeeded: fastANI
[2023-03-15 04:46:40,030] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 04:46:40,030] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 04:46:40,030] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 04:46:40,030] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 04:46:40,030] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 04:46:40,037] [INFO] DFAST Taxonomy check result was written to OceanDNA-b45445/tc_result.tsv
[2023-03-15 04:46:40,042] [INFO] ===== Taxonomy check completed =====
[2023-03-15 04:46:40,042] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 04:46:40,042] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/checkm_data
[2023-03-15 04:46:40,045] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 04:46:40,067] [INFO] Task started: CheckM
[2023-03-15 04:46:40,067] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b45445/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b45445/checkm_input OceanDNA-b45445/checkm_result
[2023-03-15 04:48:16,348] [INFO] Task succeeded: CheckM
[2023-03-15 04:48:16,348] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 04:48:16,402] [INFO] ===== Completeness check finished =====
[2023-03-15 04:48:16,402] [INFO] ===== Start GTDB Search =====
[2023-03-15 04:48:16,402] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b45445/markers.fasta)
[2023-03-15 04:48:16,403] [INFO] Task started: Blastn
[2023-03-15 04:48:16,403] [INFO] Running command: blastn -query OceanDNA-b45445/markers.fasta -db /var/lib/cwl/stg38f3aeb9-6a53-4e6c-a136-6b429b15d177/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b45445/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 04:48:17,097] [INFO] Task succeeded: Blastn
[2023-03-15 04:48:17,122] [INFO] Selected 21 target genomes.
[2023-03-15 04:48:17,123] [INFO] Target genome list was writen to OceanDNA-b45445/target_genomes_gtdb.txt
[2023-03-15 04:48:17,140] [INFO] Task started: fastANI
[2023-03-15 04:48:17,140] [INFO] Running command: fastANI --query /var/lib/cwl/stga9c1db63-0e1b-42cd-be0a-c40ad894ed99/OceanDNA-b45445.fa --refList OceanDNA-b45445/target_genomes_gtdb.txt --output OceanDNA-b45445/fastani_result_gtdb.tsv --threads 1
[2023-03-15 04:48:27,367] [INFO] Task succeeded: fastANI
[2023-03-15 04:48:27,369] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 04:48:27,369] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018668635.1	s__JABJCN01 sp018668635	99.5607	1150	1457	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Opitutales;f__JABJCN01;g__JABJCN01	95.0	99.52	99.29	0.87	0.84	6	conclusive
--------------------------------------------------------------------------------
[2023-03-15 04:48:27,370] [INFO] GTDB search result was written to OceanDNA-b45445/result_gtdb.tsv
[2023-03-15 04:48:27,371] [INFO] ===== GTDB Search completed =====
[2023-03-15 04:48:27,372] [INFO] DFAST_QC result json was written to OceanDNA-b45445/dqc_result.json
[2023-03-15 04:48:27,372] [INFO] DFAST_QC completed!
[2023-03-15 04:48:27,372] [INFO] Total running time: 0h2m37s