[2023-03-16 23:55:45,530] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:55:45,530] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:55:45,530] [INFO] DQC Reference Directory: /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference
[2023-03-16 23:55:46,667] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:55:46,667] [INFO] Task started: Prodigal
[2023-03-16 23:55:46,667] [INFO] Running command: cat /var/lib/cwl/stg94d5ae24-a310-4395-b331-63a82620c546/OceanDNA-b45854.fa | prodigal -d OceanDNA-b45854/cds.fna -a OceanDNA-b45854/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:56:09,294] [INFO] Task succeeded: Prodigal
[2023-03-16 23:56:09,294] [INFO] Task started: HMMsearch
[2023-03-16 23:56:09,294] [INFO] Running command: hmmsearch --tblout OceanDNA-b45854/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/reference_markers.hmm OceanDNA-b45854/protein.faa > /dev/null
[2023-03-16 23:56:09,479] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:56:09,480] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg94d5ae24-a310-4395-b331-63a82620c546/OceanDNA-b45854.fa]
[2023-03-16 23:56:09,503] [INFO] Query marker FASTA was written to OceanDNA-b45854/markers.fasta
[2023-03-16 23:56:09,504] [INFO] Task started: Blastn
[2023-03-16 23:56:09,504] [INFO] Running command: blastn -query OceanDNA-b45854/markers.fasta -db /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/reference_markers.fasta -out OceanDNA-b45854/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:56:10,009] [INFO] Task succeeded: Blastn
[2023-03-16 23:56:10,010] [INFO] Selected 14 target genomes.
[2023-03-16 23:56:10,010] [INFO] Target genome list was writen to OceanDNA-b45854/target_genomes.txt
[2023-03-16 23:56:10,017] [INFO] Task started: fastANI
[2023-03-16 23:56:10,017] [INFO] Running command: fastANI --query /var/lib/cwl/stg94d5ae24-a310-4395-b331-63a82620c546/OceanDNA-b45854.fa --refList OceanDNA-b45854/target_genomes.txt --output OceanDNA-b45854/fastani_result.tsv --threads 1
[2023-03-16 23:56:19,457] [INFO] Task succeeded: fastANI
[2023-03-16 23:56:19,457] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:56:19,458] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:56:19,458] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:56:19,458] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:56:19,458] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:56:19,458] [INFO] DFAST Taxonomy check result was written to OceanDNA-b45854/tc_result.tsv
[2023-03-16 23:56:19,458] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:56:19,458] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:56:19,458] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/checkm_data
[2023-03-16 23:56:19,461] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:56:19,467] [INFO] Task started: CheckM
[2023-03-16 23:56:19,467] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b45854/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b45854/checkm_input OceanDNA-b45854/checkm_result
[2023-03-16 23:57:18,325] [INFO] Task succeeded: CheckM
[2023-03-16 23:57:18,326] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 62.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 23:57:18,328] [INFO] ===== Completeness check finished =====
[2023-03-16 23:57:18,328] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:57:18,328] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b45854/markers.fasta)
[2023-03-16 23:57:18,329] [INFO] Task started: Blastn
[2023-03-16 23:57:18,329] [INFO] Running command: blastn -query OceanDNA-b45854/markers.fasta -db /var/lib/cwl/stg8f933b4b-644d-4ec6-b164-8847a7291874/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b45854/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:57:19,009] [INFO] Task succeeded: Blastn
[2023-03-16 23:57:19,010] [INFO] Selected 14 target genomes.
[2023-03-16 23:57:19,010] [INFO] Target genome list was writen to OceanDNA-b45854/target_genomes_gtdb.txt
[2023-03-16 23:57:19,575] [INFO] Task started: fastANI
[2023-03-16 23:57:19,575] [INFO] Running command: fastANI --query /var/lib/cwl/stg94d5ae24-a310-4395-b331-63a82620c546/OceanDNA-b45854.fa --refList OceanDNA-b45854/target_genomes_gtdb.txt --output OceanDNA-b45854/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:57:29,718] [INFO] Task succeeded: fastANI
[2023-03-16 23:57:29,720] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 23:57:29,720] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018402455.1	s__M30B9 sp018402455	78.8739	215	622	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Opitutales;f__Opitutaceae;g__M30B9	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018667915.1	s__M30B9 sp018667915	77.8131	151	622	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Opitutales;f__Opitutaceae;g__M30B9	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 23:57:29,721] [INFO] GTDB search result was written to OceanDNA-b45854/result_gtdb.tsv
[2023-03-16 23:57:29,721] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:57:29,721] [INFO] DFAST_QC result json was written to OceanDNA-b45854/dqc_result.json
[2023-03-16 23:57:29,722] [INFO] DFAST_QC completed!
[2023-03-16 23:57:29,722] [INFO] Total running time: 0h1m44s