[2023-03-16 11:37:45,205] [INFO] DFAST_QC pipeline started.
[2023-03-16 11:37:45,205] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 11:37:45,206] [INFO] DQC Reference Directory: /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference
[2023-03-16 11:37:47,975] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 11:37:47,975] [INFO] Task started: Prodigal
[2023-03-16 11:37:47,975] [INFO] Running command: cat /var/lib/cwl/stg18998afe-05f5-4efb-a9ae-ee152b2dce34/OceanDNA-b46295.fa | prodigal -d OceanDNA-b46295/cds.fna -a OceanDNA-b46295/protein.faa -g 11 -q > /dev/null
[2023-03-16 11:38:15,190] [INFO] Task succeeded: Prodigal
[2023-03-16 11:38:15,191] [INFO] Task started: HMMsearch
[2023-03-16 11:38:15,191] [INFO] Running command: hmmsearch --tblout OceanDNA-b46295/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/reference_markers.hmm OceanDNA-b46295/protein.faa > /dev/null
[2023-03-16 11:38:15,385] [INFO] Task succeeded: HMMsearch
[2023-03-16 11:38:15,385] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg18998afe-05f5-4efb-a9ae-ee152b2dce34/OceanDNA-b46295.fa]
[2023-03-16 11:38:15,412] [INFO] Query marker FASTA was written to OceanDNA-b46295/markers.fasta
[2023-03-16 11:38:15,412] [INFO] Task started: Blastn
[2023-03-16 11:38:15,412] [INFO] Running command: blastn -query OceanDNA-b46295/markers.fasta -db /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/reference_markers.fasta -out OceanDNA-b46295/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 11:38:15,933] [INFO] Task succeeded: Blastn
[2023-03-16 11:38:15,934] [INFO] Selected 25 target genomes.
[2023-03-16 11:38:15,934] [INFO] Target genome list was writen to OceanDNA-b46295/target_genomes.txt
[2023-03-16 11:38:15,949] [INFO] Task started: fastANI
[2023-03-16 11:38:15,949] [INFO] Running command: fastANI --query /var/lib/cwl/stg18998afe-05f5-4efb-a9ae-ee152b2dce34/OceanDNA-b46295.fa --refList OceanDNA-b46295/target_genomes.txt --output OceanDNA-b46295/fastani_result.tsv --threads 1
[2023-03-16 11:38:33,441] [INFO] Task succeeded: fastANI
[2023-03-16 11:38:33,442] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 11:38:33,442] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 11:38:33,442] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 11:38:33,442] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 11:38:33,442] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 11:38:33,442] [INFO] DFAST Taxonomy check result was written to OceanDNA-b46295/tc_result.tsv
[2023-03-16 11:38:33,442] [INFO] ===== Taxonomy check completed =====
[2023-03-16 11:38:33,443] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 11:38:33,443] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/checkm_data
[2023-03-16 11:38:33,446] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 11:38:33,451] [INFO] Task started: CheckM
[2023-03-16 11:38:33,451] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b46295/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b46295/checkm_input OceanDNA-b46295/checkm_result
[2023-03-16 11:39:41,507] [INFO] Task succeeded: CheckM
[2023-03-16 11:39:41,507] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.39%
Contamintation: 1.04%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 11:39:41,511] [INFO] ===== Completeness check finished =====
[2023-03-16 11:39:41,511] [INFO] ===== Start GTDB Search =====
[2023-03-16 11:39:41,511] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b46295/markers.fasta)
[2023-03-16 11:39:41,512] [INFO] Task started: Blastn
[2023-03-16 11:39:41,512] [INFO] Running command: blastn -query OceanDNA-b46295/markers.fasta -db /var/lib/cwl/stgf70b02da-8c0f-4d28-8a2a-bd7ef9d0ca84/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b46295/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 11:39:43,526] [INFO] Task succeeded: Blastn
[2023-03-16 11:39:43,527] [INFO] Selected 29 target genomes.
[2023-03-16 11:39:43,528] [INFO] Target genome list was writen to OceanDNA-b46295/target_genomes_gtdb.txt
[2023-03-16 11:39:44,755] [INFO] Task started: fastANI
[2023-03-16 11:39:44,755] [INFO] Running command: fastANI --query /var/lib/cwl/stg18998afe-05f5-4efb-a9ae-ee152b2dce34/OceanDNA-b46295.fa --refList OceanDNA-b46295/target_genomes_gtdb.txt --output OceanDNA-b46295/fastani_result_gtdb.tsv --threads 1
[2023-03-16 11:40:08,926] [INFO] Task succeeded: fastANI
[2023-03-16 11:40:08,926] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 11:40:08,926] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-16 11:40:08,926] [INFO] GTDB search result was written to OceanDNA-b46295/result_gtdb.tsv
[2023-03-16 11:40:08,927] [INFO] ===== GTDB Search completed =====
[2023-03-16 11:40:08,927] [INFO] DFAST_QC result json was written to OceanDNA-b46295/dqc_result.json
[2023-03-16 11:40:08,927] [INFO] DFAST_QC completed!
[2023-03-16 11:40:08,927] [INFO] Total running time: 0h2m24s