[2023-03-18 07:54:29,687] [INFO] DFAST_QC pipeline started.
[2023-03-18 07:54:29,688] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 07:54:29,688] [INFO] DQC Reference Directory: /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference
[2023-03-18 07:54:30,783] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 07:54:30,783] [INFO] Task started: Prodigal
[2023-03-18 07:54:30,784] [INFO] Running command: cat /var/lib/cwl/stg3b64d03a-89b9-4c51-a374-307d6be03991/OceanDNA-b46534.fa | prodigal -d OceanDNA-b46534/cds.fna -a OceanDNA-b46534/protein.faa -g 11 -q > /dev/null
[2023-03-18 07:54:49,306] [INFO] Task succeeded: Prodigal
[2023-03-18 07:54:49,306] [INFO] Task started: HMMsearch
[2023-03-18 07:54:49,306] [INFO] Running command: hmmsearch --tblout OceanDNA-b46534/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/reference_markers.hmm OceanDNA-b46534/protein.faa > /dev/null
[2023-03-18 07:54:49,461] [INFO] Task succeeded: HMMsearch
[2023-03-18 07:54:49,462] [INFO] Found 6/6 markers.
[2023-03-18 07:54:49,480] [INFO] Query marker FASTA was written to OceanDNA-b46534/markers.fasta
[2023-03-18 07:54:49,480] [INFO] Task started: Blastn
[2023-03-18 07:54:49,480] [INFO] Running command: blastn -query OceanDNA-b46534/markers.fasta -db /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/reference_markers.fasta -out OceanDNA-b46534/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:54:50,040] [INFO] Task succeeded: Blastn
[2023-03-18 07:54:50,042] [INFO] Selected 20 target genomes.
[2023-03-18 07:54:50,042] [INFO] Target genome list was writen to OceanDNA-b46534/target_genomes.txt
[2023-03-18 07:54:50,054] [INFO] Task started: fastANI
[2023-03-18 07:54:50,054] [INFO] Running command: fastANI --query /var/lib/cwl/stg3b64d03a-89b9-4c51-a374-307d6be03991/OceanDNA-b46534.fa --refList OceanDNA-b46534/target_genomes.txt --output OceanDNA-b46534/fastani_result.tsv --threads 1
[2023-03-18 07:55:03,810] [INFO] Task succeeded: fastANI
[2023-03-18 07:55:03,811] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 07:55:03,811] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 07:55:03,811] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 07:55:03,811] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 07:55:03,811] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 07:55:03,813] [INFO] DFAST Taxonomy check result was written to OceanDNA-b46534/tc_result.tsv
[2023-03-18 07:55:03,813] [INFO] ===== Taxonomy check completed =====
[2023-03-18 07:55:03,813] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 07:55:03,813] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/checkm_data
[2023-03-18 07:55:03,816] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 07:55:03,821] [INFO] Task started: CheckM
[2023-03-18 07:55:03,821] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b46534/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b46534/checkm_input OceanDNA-b46534/checkm_result
[2023-03-18 07:55:52,784] [INFO] Task succeeded: CheckM
[2023-03-18 07:55:52,784] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.37%
Contamintation: 5.15%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 07:55:52,786] [INFO] ===== Completeness check finished =====
[2023-03-18 07:55:52,786] [INFO] ===== Start GTDB Search =====
[2023-03-18 07:55:52,787] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b46534/markers.fasta)
[2023-03-18 07:55:52,787] [INFO] Task started: Blastn
[2023-03-18 07:55:52,787] [INFO] Running command: blastn -query OceanDNA-b46534/markers.fasta -db /var/lib/cwl/stg43273a7d-b194-4b1b-8f89-8a1424f5f531/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b46534/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:55:53,681] [INFO] Task succeeded: Blastn
[2023-03-18 07:55:53,682] [INFO] Selected 27 target genomes.
[2023-03-18 07:55:53,682] [INFO] Target genome list was writen to OceanDNA-b46534/target_genomes_gtdb.txt
[2023-03-18 07:55:53,794] [INFO] Task started: fastANI
[2023-03-18 07:55:53,794] [INFO] Running command: fastANI --query /var/lib/cwl/stg3b64d03a-89b9-4c51-a374-307d6be03991/OceanDNA-b46534.fa --refList OceanDNA-b46534/target_genomes_gtdb.txt --output OceanDNA-b46534/fastani_result_gtdb.tsv --threads 1
[2023-03-18 07:56:10,155] [INFO] Task succeeded: fastANI
[2023-03-18 07:56:10,160] [INFO] Found 7 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 07:56:10,161] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002724615.1	s__UBA6614 sp002724615	87.8501	115	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA6614	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002721895.1	s__UBA6614 sp002721895	80.4548	167	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA6614	95.0	97.45	95.11	0.88	0.86	3	-
GCA_002718935.1	s__UBA1100 sp002718935	78.1077	70	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA1100	95.0	N/A	N/A	N/A	N/A	1	-
GCA_011523245.1	s__UBA1100 sp011523245	76.9584	53	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA1100	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015660155.1	s__JACNJL01 sp015660155	76.4225	54	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__JACNJL01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009695465.1	s__SYLI01 sp009695465	76.3331	70	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__SYLI01	95.0	99.82	99.82	0.91	0.91	2	-
GCA_005809105.1	s__SYLI01 sp005809105	75.7415	55	568	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__SYLI01	95.0	99.90	99.86	0.97	0.96	4	-
--------------------------------------------------------------------------------
[2023-03-18 07:56:10,161] [INFO] GTDB search result was written to OceanDNA-b46534/result_gtdb.tsv
[2023-03-18 07:56:10,161] [INFO] ===== GTDB Search completed =====
[2023-03-18 07:56:10,162] [INFO] DFAST_QC result json was written to OceanDNA-b46534/dqc_result.json
[2023-03-18 07:56:10,162] [INFO] DFAST_QC completed!
[2023-03-18 07:56:10,162] [INFO] Total running time: 0h1m40s