[2023-03-16 18:36:54,194] [INFO] DFAST_QC pipeline started.
[2023-03-16 18:36:54,194] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 18:36:54,194] [INFO] DQC Reference Directory: /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference
[2023-03-16 18:36:56,433] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 18:36:56,434] [INFO] Task started: Prodigal
[2023-03-16 18:36:56,434] [INFO] Running command: cat /var/lib/cwl/stg11e5999c-968b-40f3-8fa7-38010a33d82b/OceanDNA-b46554.fa | prodigal -d OceanDNA-b46554/cds.fna -a OceanDNA-b46554/protein.faa -g 11 -q > /dev/null
[2023-03-16 18:37:16,910] [INFO] Task succeeded: Prodigal
[2023-03-16 18:37:16,910] [INFO] Task started: HMMsearch
[2023-03-16 18:37:16,910] [INFO] Running command: hmmsearch --tblout OceanDNA-b46554/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/reference_markers.hmm OceanDNA-b46554/protein.faa > /dev/null
[2023-03-16 18:37:17,080] [INFO] Task succeeded: HMMsearch
[2023-03-16 18:37:17,081] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg11e5999c-968b-40f3-8fa7-38010a33d82b/OceanDNA-b46554.fa]
[2023-03-16 18:37:17,098] [INFO] Query marker FASTA was written to OceanDNA-b46554/markers.fasta
[2023-03-16 18:37:17,098] [INFO] Task started: Blastn
[2023-03-16 18:37:17,098] [INFO] Running command: blastn -query OceanDNA-b46554/markers.fasta -db /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/reference_markers.fasta -out OceanDNA-b46554/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 18:37:17,618] [INFO] Task succeeded: Blastn
[2023-03-16 18:37:17,619] [INFO] Selected 15 target genomes.
[2023-03-16 18:37:17,619] [INFO] Target genome list was writen to OceanDNA-b46554/target_genomes.txt
[2023-03-16 18:37:17,654] [INFO] Task started: fastANI
[2023-03-16 18:37:17,654] [INFO] Running command: fastANI --query /var/lib/cwl/stg11e5999c-968b-40f3-8fa7-38010a33d82b/OceanDNA-b46554.fa --refList OceanDNA-b46554/target_genomes.txt --output OceanDNA-b46554/fastani_result.tsv --threads 1
[2023-03-16 18:37:31,485] [INFO] Task succeeded: fastANI
[2023-03-16 18:37:31,486] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 18:37:31,486] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 18:37:31,486] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 18:37:31,486] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 18:37:31,486] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 18:37:31,486] [INFO] DFAST Taxonomy check result was written to OceanDNA-b46554/tc_result.tsv
[2023-03-16 18:37:31,487] [INFO] ===== Taxonomy check completed =====
[2023-03-16 18:37:31,487] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 18:37:31,487] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/checkm_data
[2023-03-16 18:37:31,490] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 18:37:31,499] [INFO] Task started: CheckM
[2023-03-16 18:37:31,499] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b46554/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b46554/checkm_input OceanDNA-b46554/checkm_result
[2023-03-16 18:38:40,768] [INFO] Task succeeded: CheckM
[2023-03-16 18:38:40,769] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 73.96%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 18:38:40,771] [INFO] ===== Completeness check finished =====
[2023-03-16 18:38:40,771] [INFO] ===== Start GTDB Search =====
[2023-03-16 18:38:40,771] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b46554/markers.fasta)
[2023-03-16 18:38:40,771] [INFO] Task started: Blastn
[2023-03-16 18:38:40,771] [INFO] Running command: blastn -query OceanDNA-b46554/markers.fasta -db /var/lib/cwl/stg0c265703-fcff-48d8-97d3-10455229867b/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b46554/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 18:38:41,180] [INFO] Task succeeded: Blastn
[2023-03-16 18:38:41,181] [INFO] Selected 15 target genomes.
[2023-03-16 18:38:41,181] [INFO] Target genome list was writen to OceanDNA-b46554/target_genomes_gtdb.txt
[2023-03-16 18:38:41,217] [INFO] Task started: fastANI
[2023-03-16 18:38:41,217] [INFO] Running command: fastANI --query /var/lib/cwl/stg11e5999c-968b-40f3-8fa7-38010a33d82b/OceanDNA-b46554.fa --refList OceanDNA-b46554/target_genomes_gtdb.txt --output OceanDNA-b46554/fastani_result_gtdb.tsv --threads 1
[2023-03-16 18:38:48,347] [INFO] Task succeeded: fastANI
[2023-03-16 18:38:48,351] [INFO] Found 5 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 18:38:48,351] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015660155.1	s__JACNJL01 sp015660155	77.6058	93	726	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__JACNJL01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014382585.1	s__JACNJL01 sp014382585	76.8145	126	726	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__JACNJL01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002718935.1	s__UBA1100 sp002718935	76.601	77	726	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA1100	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003517285.1	s__UBA1100 sp003517285	76.4483	76	726	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA1100	95.0	N/A	N/A	N/A	N/A	1	-
GCA_011523245.1	s__UBA1100 sp011523245	76.4427	68	726	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA1100;g__UBA1100	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 18:38:48,351] [INFO] GTDB search result was written to OceanDNA-b46554/result_gtdb.tsv
[2023-03-16 18:38:48,352] [INFO] ===== GTDB Search completed =====
[2023-03-16 18:38:48,352] [INFO] DFAST_QC result json was written to OceanDNA-b46554/dqc_result.json
[2023-03-16 18:38:48,352] [INFO] DFAST_QC completed!
[2023-03-16 18:38:48,353] [INFO] Total running time: 0h1m54s