[2023-03-16 20:07:31,796] [INFO] DFAST_QC pipeline started.
[2023-03-16 20:07:31,796] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 20:07:31,796] [INFO] DQC Reference Directory: /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference
[2023-03-16 20:07:32,925] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 20:07:32,926] [INFO] Task started: Prodigal
[2023-03-16 20:07:32,926] [INFO] Running command: cat /var/lib/cwl/stga7c81724-c564-4047-8706-b6e3f429700e/OceanDNA-b467.fa | prodigal -d OceanDNA-b467/cds.fna -a OceanDNA-b467/protein.faa -g 11 -q > /dev/null
[2023-03-16 20:07:55,130] [INFO] Task succeeded: Prodigal
[2023-03-16 20:07:55,130] [INFO] Task started: HMMsearch
[2023-03-16 20:07:55,130] [INFO] Running command: hmmsearch --tblout OceanDNA-b467/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/reference_markers.hmm OceanDNA-b467/protein.faa > /dev/null
[2023-03-16 20:07:55,335] [INFO] Task succeeded: HMMsearch
[2023-03-16 20:07:55,335] [INFO] Found 6/6 markers.
[2023-03-16 20:07:55,360] [INFO] Query marker FASTA was written to OceanDNA-b467/markers.fasta
[2023-03-16 20:07:55,360] [INFO] Task started: Blastn
[2023-03-16 20:07:55,360] [INFO] Running command: blastn -query OceanDNA-b467/markers.fasta -db /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/reference_markers.fasta -out OceanDNA-b467/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 20:07:56,064] [INFO] Task succeeded: Blastn
[2023-03-16 20:07:56,066] [INFO] Selected 15 target genomes.
[2023-03-16 20:07:56,066] [INFO] Target genome list was writen to OceanDNA-b467/target_genomes.txt
[2023-03-16 20:07:56,074] [INFO] Task started: fastANI
[2023-03-16 20:07:56,074] [INFO] Running command: fastANI --query /var/lib/cwl/stga7c81724-c564-4047-8706-b6e3f429700e/OceanDNA-b467.fa --refList OceanDNA-b467/target_genomes.txt --output OceanDNA-b467/fastani_result.tsv --threads 1
[2023-03-16 20:08:07,559] [INFO] Task succeeded: fastANI
[2023-03-16 20:08:07,559] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 20:08:07,559] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 20:08:07,565] [INFO] Found 7 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 20:08:07,565] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-16 20:08:07,565] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Ilumatobacter coccineus	strain=YM16-304	GCA_000348785.1	467094	467094	type	True	77.9898	410	1129	95	below_threshold
Ilumatobacter nonamiensis	strain=YM16-303	GCA_000350145.1	467093	467093	type	True	77.5554	297	1129	95	below_threshold
Ilumatobacter fluminis	strain=DSM 18936	GCA_004364865.1	467091	467091	type	True	77.1088	226	1129	95	below_threshold
Desertimonas flava	strain=SYSU D60003	GCA_003426815.1	2064846	2064846	type	True	76.2421	181	1129	95	below_threshold
Actinomarinicola tropica	strain=SCSIO 58843	GCA_009650215.1	2789776	2789776	type	True	76.0571	90	1129	95	below_threshold
Rhabdothermincola salaria	strain=EGI L10124	GCA_021246445.1	2903142	2903142	type	True	75.8227	80	1129	95	below_threshold
Cryptosporangium phraense	strain=A-T 5661	GCA_006912135.1	2593070	2593070	type	True	75.0929	62	1129	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-16 20:08:07,566] [INFO] DFAST Taxonomy check result was written to OceanDNA-b467/tc_result.tsv
[2023-03-16 20:08:07,568] [INFO] ===== Taxonomy check completed =====
[2023-03-16 20:08:07,568] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 20:08:07,568] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/checkm_data
[2023-03-16 20:08:07,569] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 20:08:07,600] [INFO] Task started: CheckM
[2023-03-16 20:08:07,600] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b467/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b467/checkm_input OceanDNA-b467/checkm_result
[2023-03-16 20:09:19,213] [INFO] Task succeeded: CheckM
[2023-03-16 20:09:19,213] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 20:09:19,216] [INFO] ===== Completeness check finished =====
[2023-03-16 20:09:19,217] [INFO] ===== Start GTDB Search =====
[2023-03-16 20:09:19,217] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b467/markers.fasta)
[2023-03-16 20:09:19,218] [INFO] Task started: Blastn
[2023-03-16 20:09:19,218] [INFO] Running command: blastn -query OceanDNA-b467/markers.fasta -db /var/lib/cwl/stg5767cf4a-f3b4-4b9d-a856-758ef482d578/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b467/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 20:09:20,591] [INFO] Task succeeded: Blastn
[2023-03-16 20:09:20,593] [INFO] Selected 16 target genomes.
[2023-03-16 20:09:20,593] [INFO] Target genome list was writen to OceanDNA-b467/target_genomes_gtdb.txt
[2023-03-16 20:09:20,608] [INFO] Task started: fastANI
[2023-03-16 20:09:20,608] [INFO] Running command: fastANI --query /var/lib/cwl/stga7c81724-c564-4047-8706-b6e3f429700e/OceanDNA-b467.fa --refList OceanDNA-b467/target_genomes_gtdb.txt --output OceanDNA-b467/fastani_result_gtdb.tsv --threads 1
[2023-03-16 20:09:33,834] [INFO] Task succeeded: fastANI
[2023-03-16 20:09:33,843] [INFO] Found 14 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 20:09:33,843] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_905182555.1	s__Ilumatobacter_A sp905182555	86.8693	721	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018666235.1	s__Ilumatobacter_A sp018666235	78.5709	450	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	99.79	99.78	0.94	0.93	4	-
GCF_000348785.1	s__Ilumatobacter_A coccineus	77.9898	410	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013002175.1	s__Ilumatobacter_A sp013002175	77.8657	323	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000350145.1	s__Ilumatobacter_A nonamiensis	77.5554	297	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002711735.1	s__Ilumatobacter_A sp002711735	77.3707	169	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	99.50	99.50	0.82	0.82	2	-
GCF_004364865.1	s__Ilumatobacter fluminis	77.1085	226	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013213745.1	s__JABSQH01 sp013213745	76.9005	191	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__JABSQH01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905480335.1	s__Ilumatobacter_A sp905480335	76.6353	175	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Ilumatobacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015767405.1	s__Sva-07 sp015767405	76.6027	152	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Sva-07	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009704985.1	s__VFMC01 sp009704985	76.3316	176	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__VFMC01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016213625.1	s__UBA668 sp016213625	76.2159	157	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__UBA668	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016700055.1	s__Kalu-18 sp016700055	76.0261	135	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__Kalu-18	95.0	N/A	N/A	N/A	N/A	1	-
GCA_005778215.1	s__UBA668 sp005778215	75.8946	108	1129	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__UBA668	95.0	99.54	99.53	0.90	0.89	3	-
--------------------------------------------------------------------------------
[2023-03-16 20:09:33,844] [INFO] GTDB search result was written to OceanDNA-b467/result_gtdb.tsv
[2023-03-16 20:09:33,844] [INFO] ===== GTDB Search completed =====
[2023-03-16 20:09:33,845] [INFO] DFAST_QC result json was written to OceanDNA-b467/dqc_result.json
[2023-03-16 20:09:33,845] [INFO] DFAST_QC completed!
[2023-03-16 20:09:33,845] [INFO] Total running time: 0h2m2s