[2023-03-16 02:38:04,889] [INFO] DFAST_QC pipeline started.
[2023-03-16 02:38:04,890] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 02:38:04,890] [INFO] DQC Reference Directory: /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference
[2023-03-16 02:38:06,110] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 02:38:06,111] [INFO] Task started: Prodigal
[2023-03-16 02:38:06,111] [INFO] Running command: cat /var/lib/cwl/stgfb35d007-0531-4123-8338-19b6a3ae89e6/OceanDNA-b46877.fa | prodigal -d OceanDNA-b46877/cds.fna -a OceanDNA-b46877/protein.faa -g 11 -q > /dev/null
[2023-03-16 02:38:14,887] [INFO] Task succeeded: Prodigal
[2023-03-16 02:38:14,887] [INFO] Task started: HMMsearch
[2023-03-16 02:38:14,887] [INFO] Running command: hmmsearch --tblout OceanDNA-b46877/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/reference_markers.hmm OceanDNA-b46877/protein.faa > /dev/null
[2023-03-16 02:38:15,064] [INFO] Task succeeded: HMMsearch
[2023-03-16 02:38:15,064] [INFO] Found 6/6 markers.
[2023-03-16 02:38:15,074] [INFO] Query marker FASTA was written to OceanDNA-b46877/markers.fasta
[2023-03-16 02:38:15,074] [INFO] Task started: Blastn
[2023-03-16 02:38:15,074] [INFO] Running command: blastn -query OceanDNA-b46877/markers.fasta -db /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/reference_markers.fasta -out OceanDNA-b46877/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 02:38:15,593] [INFO] Task succeeded: Blastn
[2023-03-16 02:38:15,594] [INFO] Selected 16 target genomes.
[2023-03-16 02:38:15,594] [INFO] Target genome list was writen to OceanDNA-b46877/target_genomes.txt
[2023-03-16 02:38:15,605] [INFO] Task started: fastANI
[2023-03-16 02:38:15,605] [INFO] Running command: fastANI --query /var/lib/cwl/stgfb35d007-0531-4123-8338-19b6a3ae89e6/OceanDNA-b46877.fa --refList OceanDNA-b46877/target_genomes.txt --output OceanDNA-b46877/fastani_result.tsv --threads 1
[2023-03-16 02:38:22,787] [INFO] Task succeeded: fastANI
[2023-03-16 02:38:22,788] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 02:38:22,788] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 02:38:22,788] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 02:38:22,788] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 02:38:22,788] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 02:38:22,788] [INFO] DFAST Taxonomy check result was written to OceanDNA-b46877/tc_result.tsv
[2023-03-16 02:38:22,788] [INFO] ===== Taxonomy check completed =====
[2023-03-16 02:38:22,788] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 02:38:22,789] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/checkm_data
[2023-03-16 02:38:22,791] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 02:38:22,794] [INFO] Task started: CheckM
[2023-03-16 02:38:22,794] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b46877/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b46877/checkm_input OceanDNA-b46877/checkm_result
[2023-03-16 02:38:49,484] [INFO] Task succeeded: CheckM
[2023-03-16 02:38:49,485] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 62.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 02:38:49,486] [INFO] ===== Completeness check finished =====
[2023-03-16 02:38:49,486] [INFO] ===== Start GTDB Search =====
[2023-03-16 02:38:49,486] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b46877/markers.fasta)
[2023-03-16 02:38:49,487] [INFO] Task started: Blastn
[2023-03-16 02:38:49,487] [INFO] Running command: blastn -query OceanDNA-b46877/markers.fasta -db /var/lib/cwl/stg6dead430-42d4-4089-a6fa-55440d11c88e/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b46877/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 02:38:50,243] [INFO] Task succeeded: Blastn
[2023-03-16 02:38:50,244] [INFO] Selected 20 target genomes.
[2023-03-16 02:38:50,244] [INFO] Target genome list was writen to OceanDNA-b46877/target_genomes_gtdb.txt
[2023-03-16 02:38:50,612] [INFO] Task started: fastANI
[2023-03-16 02:38:50,612] [INFO] Running command: fastANI --query /var/lib/cwl/stgfb35d007-0531-4123-8338-19b6a3ae89e6/OceanDNA-b46877.fa --refList OceanDNA-b46877/target_genomes_gtdb.txt --output OceanDNA-b46877/fastani_result_gtdb.tsv --threads 1
[2023-03-16 02:38:58,656] [INFO] Task succeeded: fastANI
[2023-03-16 02:38:58,659] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 02:38:58,659] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002387565.1	s__UBA4581 sp002387565	99.9513	406	425	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__UBA4581	95.0	99.92	99.92	0.95	0.95	2	conclusive
GCA_002386905.1	s__UBA4581 sp002386905	83.8099	332	425	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__UBA4581	95.0	99.78	99.78	0.92	0.92	2	-
--------------------------------------------------------------------------------
[2023-03-16 02:38:58,659] [INFO] GTDB search result was written to OceanDNA-b46877/result_gtdb.tsv
[2023-03-16 02:38:58,659] [INFO] ===== GTDB Search completed =====
[2023-03-16 02:38:58,660] [INFO] DFAST_QC result json was written to OceanDNA-b46877/dqc_result.json
[2023-03-16 02:38:58,660] [INFO] DFAST_QC completed!
[2023-03-16 02:38:58,660] [INFO] Total running time: 0h0m54s