[2023-03-16 04:24:46,341] [INFO] DFAST_QC pipeline started.
[2023-03-16 04:24:46,341] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 04:24:46,341] [INFO] DQC Reference Directory: /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference
[2023-03-16 04:24:48,114] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 04:24:48,114] [INFO] Task started: Prodigal
[2023-03-16 04:24:48,114] [INFO] Running command: cat /var/lib/cwl/stg9af6008b-a8e5-4e9a-87a9-dcb327c11b7d/OceanDNA-b4960.fa | prodigal -d OceanDNA-b4960/cds.fna -a OceanDNA-b4960/protein.faa -g 11 -q > /dev/null
[2023-03-16 04:24:54,980] [INFO] Task succeeded: Prodigal
[2023-03-16 04:24:54,980] [INFO] Task started: HMMsearch
[2023-03-16 04:24:54,980] [INFO] Running command: hmmsearch --tblout OceanDNA-b4960/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/reference_markers.hmm OceanDNA-b4960/protein.faa > /dev/null
[2023-03-16 04:24:55,165] [INFO] Task succeeded: HMMsearch
[2023-03-16 04:24:55,166] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg9af6008b-a8e5-4e9a-87a9-dcb327c11b7d/OceanDNA-b4960.fa]
[2023-03-16 04:24:55,178] [INFO] Query marker FASTA was written to OceanDNA-b4960/markers.fasta
[2023-03-16 04:24:55,179] [INFO] Task started: Blastn
[2023-03-16 04:24:55,179] [INFO] Running command: blastn -query OceanDNA-b4960/markers.fasta -db /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/reference_markers.fasta -out OceanDNA-b4960/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 04:24:55,693] [INFO] Task succeeded: Blastn
[2023-03-16 04:24:55,694] [INFO] Selected 29 target genomes.
[2023-03-16 04:24:55,695] [INFO] Target genome list was writen to OceanDNA-b4960/target_genomes.txt
[2023-03-16 04:24:55,708] [INFO] Task started: fastANI
[2023-03-16 04:24:55,708] [INFO] Running command: fastANI --query /var/lib/cwl/stg9af6008b-a8e5-4e9a-87a9-dcb327c11b7d/OceanDNA-b4960.fa --refList OceanDNA-b4960/target_genomes.txt --output OceanDNA-b4960/fastani_result.tsv --threads 1
[2023-03-16 04:25:11,207] [INFO] Task succeeded: fastANI
[2023-03-16 04:25:11,208] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 04:25:11,208] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 04:25:11,208] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 04:25:11,208] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 04:25:11,208] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 04:25:11,208] [INFO] DFAST Taxonomy check result was written to OceanDNA-b4960/tc_result.tsv
[2023-03-16 04:25:11,208] [INFO] ===== Taxonomy check completed =====
[2023-03-16 04:25:11,208] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 04:25:11,209] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/checkm_data
[2023-03-16 04:25:11,212] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 04:25:11,215] [INFO] Task started: CheckM
[2023-03-16 04:25:11,215] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b4960/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b4960/checkm_input OceanDNA-b4960/checkm_result
[2023-03-16 04:25:34,346] [INFO] Task succeeded: CheckM
[2023-03-16 04:25:34,347] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 73.46%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 04:25:34,349] [INFO] ===== Completeness check finished =====
[2023-03-16 04:25:34,349] [INFO] ===== Start GTDB Search =====
[2023-03-16 04:25:34,350] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b4960/markers.fasta)
[2023-03-16 04:25:34,350] [INFO] Task started: Blastn
[2023-03-16 04:25:34,350] [INFO] Running command: blastn -query OceanDNA-b4960/markers.fasta -db /var/lib/cwl/stg49729ca4-6568-48b7-aace-a4e71702cfaa/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b4960/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 04:25:35,106] [INFO] Task succeeded: Blastn
[2023-03-16 04:25:35,107] [INFO] Selected 27 target genomes.
[2023-03-16 04:25:35,107] [INFO] Target genome list was writen to OceanDNA-b4960/target_genomes_gtdb.txt
[2023-03-16 04:25:35,162] [INFO] Task started: fastANI
[2023-03-16 04:25:35,162] [INFO] Running command: fastANI --query /var/lib/cwl/stg9af6008b-a8e5-4e9a-87a9-dcb327c11b7d/OceanDNA-b4960.fa --refList OceanDNA-b4960/target_genomes_gtdb.txt --output OceanDNA-b4960/fastani_result_gtdb.tsv --threads 1
[2023-03-16 04:25:46,035] [INFO] Task succeeded: fastANI
[2023-03-16 04:25:46,040] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 04:25:46,041] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002696275.1	s__GCA-002697625 sp002696275	79.8887	221	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002697625.1	s__GCA-002697625 sp002697625	78.3004	144	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902514225.1	s__GCA-002697625 sp902514225	76.9456	56	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905478555.1	s__GCA-002697625 sp905478555	76.6628	93	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	96.63	96.63	0.87	0.87	2	-
GCA_018695965.1	s__GCA-002697625 sp018695965	76.5785	70	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	98.45	97.65	0.78	0.72	6	-
GCA_002710495.1	s__GCA-002697625 sp002710495	76.4085	79	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018647945.1	s__GCA-002697625 sp018647945	76.3317	87	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__GCA-002697625	95.0	99.64	99.58	0.90	0.90	3	-
GCA_905478765.1	s__UWMA-0277 sp905478765	76.0328	55	338	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__BACL11;g__UWMA-0277	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 04:25:46,041] [INFO] GTDB search result was written to OceanDNA-b4960/result_gtdb.tsv
[2023-03-16 04:25:46,041] [INFO] ===== GTDB Search completed =====
[2023-03-16 04:25:46,042] [INFO] DFAST_QC result json was written to OceanDNA-b4960/dqc_result.json
[2023-03-16 04:25:46,042] [INFO] DFAST_QC completed!
[2023-03-16 04:25:46,042] [INFO] Total running time: 0h0m60s