[2023-03-17 01:23:45,120] [INFO] DFAST_QC pipeline started.
[2023-03-17 01:23:45,121] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 01:23:45,121] [INFO] DQC Reference Directory: /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference
[2023-03-17 01:23:46,318] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 01:23:46,318] [INFO] Task started: Prodigal
[2023-03-17 01:23:46,318] [INFO] Running command: cat /var/lib/cwl/stg2a5023b4-1824-4e17-8429-b0e0a5b4c35e/OceanDNA-b5229.fa | prodigal -d OceanDNA-b5229/cds.fna -a OceanDNA-b5229/protein.faa -g 11 -q > /dev/null
[2023-03-17 01:23:53,220] [INFO] Task succeeded: Prodigal
[2023-03-17 01:23:53,221] [INFO] Task started: HMMsearch
[2023-03-17 01:23:53,221] [INFO] Running command: hmmsearch --tblout OceanDNA-b5229/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/reference_markers.hmm OceanDNA-b5229/protein.faa > /dev/null
[2023-03-17 01:23:53,381] [INFO] Task succeeded: HMMsearch
[2023-03-17 01:23:53,382] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg2a5023b4-1824-4e17-8429-b0e0a5b4c35e/OceanDNA-b5229.fa]
[2023-03-17 01:23:53,391] [INFO] Query marker FASTA was written to OceanDNA-b5229/markers.fasta
[2023-03-17 01:23:53,391] [INFO] Task started: Blastn
[2023-03-17 01:23:53,391] [INFO] Running command: blastn -query OceanDNA-b5229/markers.fasta -db /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/reference_markers.fasta -out OceanDNA-b5229/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 01:23:53,936] [INFO] Task succeeded: Blastn
[2023-03-17 01:23:53,937] [INFO] Selected 23 target genomes.
[2023-03-17 01:23:53,937] [INFO] Target genome list was writen to OceanDNA-b5229/target_genomes.txt
[2023-03-17 01:23:53,950] [INFO] Task started: fastANI
[2023-03-17 01:23:53,951] [INFO] Running command: fastANI --query /var/lib/cwl/stg2a5023b4-1824-4e17-8429-b0e0a5b4c35e/OceanDNA-b5229.fa --refList OceanDNA-b5229/target_genomes.txt --output OceanDNA-b5229/fastani_result.tsv --threads 1
[2023-03-17 01:24:06,463] [INFO] Task succeeded: fastANI
[2023-03-17 01:24:06,464] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 01:24:06,464] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 01:24:06,464] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 01:24:06,464] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 01:24:06,464] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 01:24:06,464] [INFO] DFAST Taxonomy check result was written to OceanDNA-b5229/tc_result.tsv
[2023-03-17 01:24:06,464] [INFO] ===== Taxonomy check completed =====
[2023-03-17 01:24:06,464] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 01:24:06,465] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/checkm_data
[2023-03-17 01:24:06,467] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 01:24:06,471] [INFO] Task started: CheckM
[2023-03-17 01:24:06,471] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b5229/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b5229/checkm_input OceanDNA-b5229/checkm_result
[2023-03-17 01:24:28,981] [INFO] Task succeeded: CheckM
[2023-03-17 01:24:28,981] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.67%
Contamintation: 3.12%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-17 01:24:28,983] [INFO] ===== Completeness check finished =====
[2023-03-17 01:24:28,983] [INFO] ===== Start GTDB Search =====
[2023-03-17 01:24:28,983] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b5229/markers.fasta)
[2023-03-17 01:24:28,984] [INFO] Task started: Blastn
[2023-03-17 01:24:28,984] [INFO] Running command: blastn -query OceanDNA-b5229/markers.fasta -db /var/lib/cwl/stgacf7215c-046f-47f8-9970-242259b69a15/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b5229/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 01:24:29,843] [INFO] Task succeeded: Blastn
[2023-03-17 01:24:29,844] [INFO] Selected 23 target genomes.
[2023-03-17 01:24:29,844] [INFO] Target genome list was writen to OceanDNA-b5229/target_genomes_gtdb.txt
[2023-03-17 01:24:30,619] [INFO] Task started: fastANI
[2023-03-17 01:24:30,619] [INFO] Running command: fastANI --query /var/lib/cwl/stg2a5023b4-1824-4e17-8429-b0e0a5b4c35e/OceanDNA-b5229.fa --refList OceanDNA-b5229/target_genomes_gtdb.txt --output OceanDNA-b5229/fastani_result_gtdb.tsv --threads 1
[2023-03-17 01:24:37,769] [INFO] Task succeeded: fastANI
[2023-03-17 01:24:37,772] [INFO] Found 4 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 01:24:37,772] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002714805.1	s__UBA8444 sp002714805	86.4683	293	357	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UBA7312;g__UBA8444	95.0	95.59	95.59	0.86	0.86	2	-
GCA_003454845.1	s__UBA8444 sp003454845	83.3379	124	357	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UBA7312;g__UBA8444	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016779995.1	s__UBA8444 sp016779995	83.0866	240	357	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UBA7312;g__UBA8444	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016779935.1	s__GCA-2862585 sp016779935	76.9709	83	357	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UBA7312;g__GCA-2862585	95.0	99.89	99.89	0.93	0.93	2	-
--------------------------------------------------------------------------------
[2023-03-17 01:24:37,772] [INFO] GTDB search result was written to OceanDNA-b5229/result_gtdb.tsv
[2023-03-17 01:24:37,773] [INFO] ===== GTDB Search completed =====
[2023-03-17 01:24:37,773] [INFO] DFAST_QC result json was written to OceanDNA-b5229/dqc_result.json
[2023-03-17 01:24:37,773] [INFO] DFAST_QC completed!
[2023-03-17 01:24:37,773] [INFO] Total running time: 0h0m53s