[2023-03-18 23:31:26,226] [INFO] DFAST_QC pipeline started.
[2023-03-18 23:31:26,226] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 23:31:26,227] [INFO] DQC Reference Directory: /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference
[2023-03-18 23:31:27,313] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 23:31:27,313] [INFO] Task started: Prodigal
[2023-03-18 23:31:27,314] [INFO] Running command: cat /var/lib/cwl/stg1a85f2a3-afe6-4d96-bf49-68d1560c8d50/OceanDNA-b5592.fa | prodigal -d OceanDNA-b5592/cds.fna -a OceanDNA-b5592/protein.faa -g 11 -q > /dev/null
[2023-03-18 23:31:40,938] [INFO] Task succeeded: Prodigal
[2023-03-18 23:31:40,939] [INFO] Task started: HMMsearch
[2023-03-18 23:31:40,939] [INFO] Running command: hmmsearch --tblout OceanDNA-b5592/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/reference_markers.hmm OceanDNA-b5592/protein.faa > /dev/null
[2023-03-18 23:31:41,120] [INFO] Task succeeded: HMMsearch
[2023-03-18 23:31:41,121] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg1a85f2a3-afe6-4d96-bf49-68d1560c8d50/OceanDNA-b5592.fa]
[2023-03-18 23:31:41,135] [INFO] Query marker FASTA was written to OceanDNA-b5592/markers.fasta
[2023-03-18 23:31:41,136] [INFO] Task started: Blastn
[2023-03-18 23:31:41,136] [INFO] Running command: blastn -query OceanDNA-b5592/markers.fasta -db /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/reference_markers.fasta -out OceanDNA-b5592/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:31:41,676] [INFO] Task succeeded: Blastn
[2023-03-18 23:31:41,676] [INFO] Selected 21 target genomes.
[2023-03-18 23:31:41,677] [INFO] Target genome list was writen to OceanDNA-b5592/target_genomes.txt
[2023-03-18 23:31:41,685] [INFO] Task started: fastANI
[2023-03-18 23:31:41,685] [INFO] Running command: fastANI --query /var/lib/cwl/stg1a85f2a3-afe6-4d96-bf49-68d1560c8d50/OceanDNA-b5592.fa --refList OceanDNA-b5592/target_genomes.txt --output OceanDNA-b5592/fastani_result.tsv --threads 1
[2023-03-18 23:31:54,277] [INFO] Task succeeded: fastANI
[2023-03-18 23:31:54,277] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 23:31:54,277] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 23:31:54,277] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 23:31:54,277] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 23:31:54,277] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 23:31:54,278] [INFO] DFAST Taxonomy check result was written to OceanDNA-b5592/tc_result.tsv
[2023-03-18 23:31:54,278] [INFO] ===== Taxonomy check completed =====
[2023-03-18 23:31:54,278] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 23:31:54,278] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/checkm_data
[2023-03-18 23:31:54,281] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 23:31:54,340] [INFO] Task started: CheckM
[2023-03-18 23:31:54,340] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b5592/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b5592/checkm_input OceanDNA-b5592/checkm_result
[2023-03-18 23:32:33,453] [INFO] Task succeeded: CheckM
[2023-03-18 23:32:33,453] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 7.23%
Strain heterogeneity: 75.00%
--------------------------------------------------------------------------------
[2023-03-18 23:32:33,456] [INFO] ===== Completeness check finished =====
[2023-03-18 23:32:33,456] [INFO] ===== Start GTDB Search =====
[2023-03-18 23:32:33,456] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b5592/markers.fasta)
[2023-03-18 23:32:33,457] [INFO] Task started: Blastn
[2023-03-18 23:32:33,457] [INFO] Running command: blastn -query OceanDNA-b5592/markers.fasta -db /var/lib/cwl/stg6da7f912-99c3-468b-995d-4d440f1b650f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b5592/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:32:34,280] [INFO] Task succeeded: Blastn
[2023-03-18 23:32:34,281] [INFO] Selected 27 target genomes.
[2023-03-18 23:32:34,281] [INFO] Target genome list was writen to OceanDNA-b5592/target_genomes_gtdb.txt
[2023-03-18 23:32:34,297] [INFO] Task started: fastANI
[2023-03-18 23:32:34,297] [INFO] Running command: fastANI --query /var/lib/cwl/stg1a85f2a3-afe6-4d96-bf49-68d1560c8d50/OceanDNA-b5592.fa --refList OceanDNA-b5592/target_genomes_gtdb.txt --output OceanDNA-b5592/fastani_result_gtdb.tsv --threads 1
[2023-03-18 23:32:46,288] [INFO] Task succeeded: fastANI
[2023-03-18 23:32:46,290] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 23:32:46,290] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009921445.1	s__RFXV01 sp009921445	93.4431	340	434	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Schleiferiaceae;g__RFXV01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 23:32:46,290] [INFO] GTDB search result was written to OceanDNA-b5592/result_gtdb.tsv
[2023-03-18 23:32:46,291] [INFO] ===== GTDB Search completed =====
[2023-03-18 23:32:46,291] [INFO] DFAST_QC result json was written to OceanDNA-b5592/dqc_result.json
[2023-03-18 23:32:46,291] [INFO] DFAST_QC completed!
[2023-03-18 23:32:46,291] [INFO] Total running time: 0h1m20s