[2023-03-17 14:51:39,396] [INFO] DFAST_QC pipeline started.
[2023-03-17 14:51:39,396] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 14:51:39,396] [INFO] DQC Reference Directory: /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference
[2023-03-17 14:51:40,528] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 14:51:40,529] [INFO] Task started: Prodigal
[2023-03-17 14:51:40,529] [INFO] Running command: cat /var/lib/cwl/stgc2b4cdf6-d6fa-4736-a55a-8bda696a5e2d/OceanDNA-b57.fa | prodigal -d OceanDNA-b57/cds.fna -a OceanDNA-b57/protein.faa -g 11 -q > /dev/null
[2023-03-17 14:51:49,628] [INFO] Task succeeded: Prodigal
[2023-03-17 14:51:49,628] [INFO] Task started: HMMsearch
[2023-03-17 14:51:49,628] [INFO] Running command: hmmsearch --tblout OceanDNA-b57/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/reference_markers.hmm OceanDNA-b57/protein.faa > /dev/null
[2023-03-17 14:51:49,984] [INFO] Task succeeded: HMMsearch
[2023-03-17 14:51:49,985] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgc2b4cdf6-d6fa-4736-a55a-8bda696a5e2d/OceanDNA-b57.fa]
[2023-03-17 14:51:50,013] [INFO] Query marker FASTA was written to OceanDNA-b57/markers.fasta
[2023-03-17 14:51:50,014] [INFO] Task started: Blastn
[2023-03-17 14:51:50,014] [INFO] Running command: blastn -query OceanDNA-b57/markers.fasta -db /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/reference_markers.fasta -out OceanDNA-b57/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 14:51:51,502] [INFO] Task succeeded: Blastn
[2023-03-17 14:51:51,503] [INFO] Selected 18 target genomes.
[2023-03-17 14:51:51,503] [INFO] Target genome list was writen to OceanDNA-b57/target_genomes.txt
[2023-03-17 14:51:51,514] [INFO] Task started: fastANI
[2023-03-17 14:51:51,515] [INFO] Running command: fastANI --query /var/lib/cwl/stgc2b4cdf6-d6fa-4736-a55a-8bda696a5e2d/OceanDNA-b57.fa --refList OceanDNA-b57/target_genomes.txt --output OceanDNA-b57/fastani_result.tsv --threads 1
[2023-03-17 14:52:11,425] [INFO] Task succeeded: fastANI
[2023-03-17 14:52:11,425] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 14:52:11,425] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 14:52:11,426] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 14:52:11,426] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 14:52:11,426] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 14:52:11,426] [INFO] DFAST Taxonomy check result was written to OceanDNA-b57/tc_result.tsv
[2023-03-17 14:52:11,426] [INFO] ===== Taxonomy check completed =====
[2023-03-17 14:52:11,426] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 14:52:11,426] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/checkm_data
[2023-03-17 14:52:11,429] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 14:52:11,432] [INFO] Task started: CheckM
[2023-03-17 14:52:11,432] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b57/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b57/checkm_input OceanDNA-b57/checkm_result
[2023-03-17 14:52:44,749] [INFO] Task succeeded: CheckM
[2023-03-17 14:52:44,749] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 75.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 14:52:44,751] [INFO] ===== Completeness check finished =====
[2023-03-17 14:52:44,751] [INFO] ===== Start GTDB Search =====
[2023-03-17 14:52:44,751] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b57/markers.fasta)
[2023-03-17 14:52:44,752] [INFO] Task started: Blastn
[2023-03-17 14:52:44,752] [INFO] Running command: blastn -query OceanDNA-b57/markers.fasta -db /var/lib/cwl/stg624278a0-74f1-4fcb-8030-7ccf144e5584/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b57/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 14:52:46,244] [INFO] Task succeeded: Blastn
[2023-03-17 14:52:46,245] [INFO] Selected 24 target genomes.
[2023-03-17 14:52:46,245] [INFO] Target genome list was writen to OceanDNA-b57/target_genomes_gtdb.txt
[2023-03-17 14:52:46,733] [INFO] Task started: fastANI
[2023-03-17 14:52:46,733] [INFO] Running command: fastANI --query /var/lib/cwl/stgc2b4cdf6-d6fa-4736-a55a-8bda696a5e2d/OceanDNA-b57.fa --refList OceanDNA-b57/target_genomes_gtdb.txt --output OceanDNA-b57/fastani_result_gtdb.tsv --threads 1
[2023-03-17 14:53:08,433] [INFO] Task succeeded: fastANI
[2023-03-17 14:53:08,433] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 14:53:08,433] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-17 14:53:08,433] [INFO] GTDB search result was written to OceanDNA-b57/result_gtdb.tsv
[2023-03-17 14:53:08,434] [INFO] ===== GTDB Search completed =====
[2023-03-17 14:53:08,434] [INFO] DFAST_QC result json was written to OceanDNA-b57/dqc_result.json
[2023-03-17 14:53:08,434] [INFO] DFAST_QC completed!
[2023-03-17 14:53:08,434] [INFO] Total running time: 0h1m29s