[2023-03-15 09:25:29,027] [INFO] DFAST_QC pipeline started.
[2023-03-15 09:25:29,028] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 09:25:29,028] [INFO] DQC Reference Directory: /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference
[2023-03-15 09:25:30,382] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 09:25:30,382] [INFO] Task started: Prodigal
[2023-03-15 09:25:30,382] [INFO] Running command: cat /var/lib/cwl/stg8e59b38d-feb8-49ee-80e6-b5985db75790/OceanDNA-b58.fa | prodigal -d OceanDNA-b58/cds.fna -a OceanDNA-b58/protein.faa -g 11 -q > /dev/null
[2023-03-15 09:25:39,753] [INFO] Task succeeded: Prodigal
[2023-03-15 09:25:39,753] [INFO] Task started: HMMsearch
[2023-03-15 09:25:39,753] [INFO] Running command: hmmsearch --tblout OceanDNA-b58/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/reference_markers.hmm OceanDNA-b58/protein.faa > /dev/null
[2023-03-15 09:25:39,921] [INFO] Task succeeded: HMMsearch
[2023-03-15 09:25:39,922] [INFO] Found 6/6 markers.
[2023-03-15 09:25:39,935] [INFO] Query marker FASTA was written to OceanDNA-b58/markers.fasta
[2023-03-15 09:25:39,936] [INFO] Task started: Blastn
[2023-03-15 09:25:39,936] [INFO] Running command: blastn -query OceanDNA-b58/markers.fasta -db /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/reference_markers.fasta -out OceanDNA-b58/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:25:40,450] [INFO] Task succeeded: Blastn
[2023-03-15 09:25:40,451] [INFO] Selected 21 target genomes.
[2023-03-15 09:25:40,451] [INFO] Target genome list was writen to OceanDNA-b58/target_genomes.txt
[2023-03-15 09:25:40,464] [INFO] Task started: fastANI
[2023-03-15 09:25:40,464] [INFO] Running command: fastANI --query /var/lib/cwl/stg8e59b38d-feb8-49ee-80e6-b5985db75790/OceanDNA-b58.fa --refList OceanDNA-b58/target_genomes.txt --output OceanDNA-b58/fastani_result.tsv --threads 1
[2023-03-15 09:25:55,732] [INFO] Task succeeded: fastANI
[2023-03-15 09:25:55,733] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 09:25:55,733] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 09:25:55,733] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 09:25:55,733] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 09:25:55,733] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 09:25:55,734] [INFO] DFAST Taxonomy check result was written to OceanDNA-b58/tc_result.tsv
[2023-03-15 09:25:55,734] [INFO] ===== Taxonomy check completed =====
[2023-03-15 09:25:55,734] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 09:25:55,734] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/checkm_data
[2023-03-15 09:25:55,737] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 09:25:55,756] [INFO] Task started: CheckM
[2023-03-15 09:25:55,756] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b58/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b58/checkm_input OceanDNA-b58/checkm_result
[2023-03-15 09:26:24,169] [INFO] Task succeeded: CheckM
[2023-03-15 09:26:24,170] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.15%
Contamintation: 1.04%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 09:26:24,172] [INFO] ===== Completeness check finished =====
[2023-03-15 09:26:24,172] [INFO] ===== Start GTDB Search =====
[2023-03-15 09:26:24,172] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b58/markers.fasta)
[2023-03-15 09:26:24,173] [INFO] Task started: Blastn
[2023-03-15 09:26:24,173] [INFO] Running command: blastn -query OceanDNA-b58/markers.fasta -db /var/lib/cwl/stg552bb104-effe-49e0-a802-7882dd4392a0/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b58/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:26:24,896] [INFO] Task succeeded: Blastn
[2023-03-15 09:26:24,897] [INFO] Selected 29 target genomes.
[2023-03-15 09:26:24,897] [INFO] Target genome list was writen to OceanDNA-b58/target_genomes_gtdb.txt
[2023-03-15 09:26:25,607] [INFO] Task started: fastANI
[2023-03-15 09:26:25,607] [INFO] Running command: fastANI --query /var/lib/cwl/stg8e59b38d-feb8-49ee-80e6-b5985db75790/OceanDNA-b58.fa --refList OceanDNA-b58/target_genomes_gtdb.txt --output OceanDNA-b58/fastani_result_gtdb.tsv --threads 1
[2023-03-15 09:26:40,809] [INFO] Task succeeded: fastANI
[2023-03-15 09:26:40,810] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 09:26:40,810] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 09:26:40,810] [INFO] GTDB search result was written to OceanDNA-b58/result_gtdb.tsv
[2023-03-15 09:26:40,810] [INFO] ===== GTDB Search completed =====
[2023-03-15 09:26:40,811] [INFO] DFAST_QC result json was written to OceanDNA-b58/dqc_result.json
[2023-03-15 09:26:40,811] [INFO] DFAST_QC completed!
[2023-03-15 09:26:40,811] [INFO] Total running time: 0h1m12s