[2023-03-16 17:15:23,145] [INFO] DFAST_QC pipeline started.
[2023-03-16 17:15:23,145] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 17:15:23,145] [INFO] DQC Reference Directory: /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference
[2023-03-16 17:15:24,225] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 17:15:24,225] [INFO] Task started: Prodigal
[2023-03-16 17:15:24,226] [INFO] Running command: cat /var/lib/cwl/stg8ad101cd-c089-494c-b6d0-37ac6f2b9711/OceanDNA-b60.fa | prodigal -d OceanDNA-b60/cds.fna -a OceanDNA-b60/protein.faa -g 11 -q > /dev/null
[2023-03-16 17:15:38,649] [INFO] Task succeeded: Prodigal
[2023-03-16 17:15:38,649] [INFO] Task started: HMMsearch
[2023-03-16 17:15:38,649] [INFO] Running command: hmmsearch --tblout OceanDNA-b60/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/reference_markers.hmm OceanDNA-b60/protein.faa > /dev/null
[2023-03-16 17:15:38,830] [INFO] Task succeeded: HMMsearch
[2023-03-16 17:15:38,831] [INFO] Found 6/6 markers.
[2023-03-16 17:15:38,847] [INFO] Query marker FASTA was written to OceanDNA-b60/markers.fasta
[2023-03-16 17:15:38,848] [INFO] Task started: Blastn
[2023-03-16 17:15:38,848] [INFO] Running command: blastn -query OceanDNA-b60/markers.fasta -db /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/reference_markers.fasta -out OceanDNA-b60/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:15:39,364] [INFO] Task succeeded: Blastn
[2023-03-16 17:15:39,364] [INFO] Selected 10 target genomes.
[2023-03-16 17:15:39,365] [INFO] Target genome list was writen to OceanDNA-b60/target_genomes.txt
[2023-03-16 17:15:39,380] [INFO] Task started: fastANI
[2023-03-16 17:15:39,380] [INFO] Running command: fastANI --query /var/lib/cwl/stg8ad101cd-c089-494c-b6d0-37ac6f2b9711/OceanDNA-b60.fa --refList OceanDNA-b60/target_genomes.txt --output OceanDNA-b60/fastani_result.tsv --threads 1
[2023-03-16 17:15:44,656] [INFO] Task succeeded: fastANI
[2023-03-16 17:15:44,656] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 17:15:44,657] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 17:15:44,657] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 17:15:44,657] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 17:15:44,657] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 17:15:44,657] [INFO] DFAST Taxonomy check result was written to OceanDNA-b60/tc_result.tsv
[2023-03-16 17:15:44,657] [INFO] ===== Taxonomy check completed =====
[2023-03-16 17:15:44,657] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 17:15:44,658] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/checkm_data
[2023-03-16 17:15:44,660] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 17:15:44,666] [INFO] Task started: CheckM
[2023-03-16 17:15:44,666] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b60/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b60/checkm_input OceanDNA-b60/checkm_result
[2023-03-16 17:16:34,435] [INFO] Task succeeded: CheckM
[2023-03-16 17:16:34,435] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 17:16:34,438] [INFO] ===== Completeness check finished =====
[2023-03-16 17:16:34,438] [INFO] ===== Start GTDB Search =====
[2023-03-16 17:16:34,438] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b60/markers.fasta)
[2023-03-16 17:16:34,440] [INFO] Task started: Blastn
[2023-03-16 17:16:34,440] [INFO] Running command: blastn -query OceanDNA-b60/markers.fasta -db /var/lib/cwl/stg4583acc1-6bf8-4c42-bd80-0cf45d200521/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b60/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:16:35,177] [INFO] Task succeeded: Blastn
[2023-03-16 17:16:35,179] [INFO] Selected 25 target genomes.
[2023-03-16 17:16:35,179] [INFO] Target genome list was writen to OceanDNA-b60/target_genomes_gtdb.txt
[2023-03-16 17:16:35,212] [INFO] Task started: fastANI
[2023-03-16 17:16:35,212] [INFO] Running command: fastANI --query /var/lib/cwl/stg8ad101cd-c089-494c-b6d0-37ac6f2b9711/OceanDNA-b60.fa --refList OceanDNA-b60/target_genomes_gtdb.txt --output OceanDNA-b60/fastani_result_gtdb.tsv --threads 1
[2023-03-16 17:16:48,266] [INFO] Task succeeded: fastANI
[2023-03-16 17:16:48,267] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 17:16:48,267] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-16 17:16:48,267] [INFO] GTDB search result was written to OceanDNA-b60/result_gtdb.tsv
[2023-03-16 17:16:48,267] [INFO] ===== GTDB Search completed =====
[2023-03-16 17:16:48,268] [INFO] DFAST_QC result json was written to OceanDNA-b60/dqc_result.json
[2023-03-16 17:16:48,268] [INFO] DFAST_QC completed!
[2023-03-16 17:16:48,268] [INFO] Total running time: 0h1m25s