[2023-03-14 14:59:36,907] [INFO] DFAST_QC pipeline started.
[2023-03-14 14:59:36,907] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 14:59:36,907] [INFO] DQC Reference Directory: /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference
[2023-03-14 14:59:38,203] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 14:59:38,203] [INFO] Task started: Prodigal
[2023-03-14 14:59:38,203] [INFO] Running command: cat /var/lib/cwl/stge7bf2fc3-7f1b-4f55-b872-55b9bdfd8808/OceanDNA-b6102.fa | prodigal -d OceanDNA-b6102/cds.fna -a OceanDNA-b6102/protein.faa -g 11 -q > /dev/null
[2023-03-14 15:00:00,352] [INFO] Task succeeded: Prodigal
[2023-03-14 15:00:00,352] [INFO] Task started: HMMsearch
[2023-03-14 15:00:00,352] [INFO] Running command: hmmsearch --tblout OceanDNA-b6102/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/reference_markers.hmm OceanDNA-b6102/protein.faa > /dev/null
[2023-03-14 15:00:00,508] [INFO] Task succeeded: HMMsearch
[2023-03-14 15:00:00,508] [WARNING] Found 5/6 markers. [/var/lib/cwl/stge7bf2fc3-7f1b-4f55-b872-55b9bdfd8808/OceanDNA-b6102.fa]
[2023-03-14 15:00:00,521] [INFO] Query marker FASTA was written to OceanDNA-b6102/markers.fasta
[2023-03-14 15:00:00,522] [INFO] Task started: Blastn
[2023-03-14 15:00:00,522] [INFO] Running command: blastn -query OceanDNA-b6102/markers.fasta -db /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/reference_markers.fasta -out OceanDNA-b6102/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 15:00:01,034] [INFO] Task succeeded: Blastn
[2023-03-14 15:00:01,035] [INFO] Selected 16 target genomes.
[2023-03-14 15:00:01,035] [INFO] Target genome list was writen to OceanDNA-b6102/target_genomes.txt
[2023-03-14 15:00:01,043] [INFO] Task started: fastANI
[2023-03-14 15:00:01,043] [INFO] Running command: fastANI --query /var/lib/cwl/stge7bf2fc3-7f1b-4f55-b872-55b9bdfd8808/OceanDNA-b6102.fa --refList OceanDNA-b6102/target_genomes.txt --output OceanDNA-b6102/fastani_result.tsv --threads 1
[2023-03-14 15:00:10,938] [INFO] Task succeeded: fastANI
[2023-03-14 15:00:10,938] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 15:00:10,938] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 15:00:10,939] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 15:00:10,939] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 15:00:10,939] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 15:00:10,939] [INFO] DFAST Taxonomy check result was written to OceanDNA-b6102/tc_result.tsv
[2023-03-14 15:00:10,940] [INFO] ===== Taxonomy check completed =====
[2023-03-14 15:00:10,940] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 15:00:10,940] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/checkm_data
[2023-03-14 15:00:10,943] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 15:00:10,991] [INFO] Task started: CheckM
[2023-03-14 15:00:10,991] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b6102/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b6102/checkm_input OceanDNA-b6102/checkm_result
[2023-03-14 15:01:02,820] [INFO] Task succeeded: CheckM
[2023-03-14 15:01:02,820] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 15:01:02,822] [INFO] ===== Completeness check finished =====
[2023-03-14 15:01:02,823] [INFO] ===== Start GTDB Search =====
[2023-03-14 15:01:02,823] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b6102/markers.fasta)
[2023-03-14 15:01:02,824] [INFO] Task started: Blastn
[2023-03-14 15:01:02,824] [INFO] Running command: blastn -query OceanDNA-b6102/markers.fasta -db /var/lib/cwl/stgda0ef315-f334-4a93-87f4-1fc9ce68c523/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b6102/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 15:01:03,514] [INFO] Task succeeded: Blastn
[2023-03-14 15:01:03,515] [INFO] Selected 21 target genomes.
[2023-03-14 15:01:03,515] [INFO] Target genome list was writen to OceanDNA-b6102/target_genomes_gtdb.txt
[2023-03-14 15:01:03,558] [INFO] Task started: fastANI
[2023-03-14 15:01:03,558] [INFO] Running command: fastANI --query /var/lib/cwl/stge7bf2fc3-7f1b-4f55-b872-55b9bdfd8808/OceanDNA-b6102.fa --refList OceanDNA-b6102/target_genomes_gtdb.txt --output OceanDNA-b6102/fastani_result_gtdb.tsv --threads 1
[2023-03-14 15:01:13,800] [INFO] Task succeeded: fastANI
[2023-03-14 15:01:13,802] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-14 15:01:13,802] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002378565.1	s__UBA3442 sp002378565	95.2729	409	509	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Schleiferiaceae;g__UBA3442	95.0	99.19	98.61	0.89	0.85	22	conclusive
--------------------------------------------------------------------------------
[2023-03-14 15:01:13,803] [INFO] GTDB search result was written to OceanDNA-b6102/result_gtdb.tsv
[2023-03-14 15:01:13,803] [INFO] ===== GTDB Search completed =====
[2023-03-14 15:01:13,804] [INFO] DFAST_QC result json was written to OceanDNA-b6102/dqc_result.json
[2023-03-14 15:01:13,804] [INFO] DFAST_QC completed!
[2023-03-14 15:01:13,804] [INFO] Total running time: 0h1m37s