[2023-03-18 23:15:22,408] [INFO] DFAST_QC pipeline started.
[2023-03-18 23:15:22,408] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 23:15:22,409] [INFO] DQC Reference Directory: /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference
[2023-03-18 23:15:26,291] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 23:15:26,292] [INFO] Task started: Prodigal
[2023-03-18 23:15:26,292] [INFO] Running command: cat /var/lib/cwl/stgf9f40036-9821-4105-8e9d-e0e215d632e9/OceanDNA-b6587.fa | prodigal -d OceanDNA-b6587/cds.fna -a OceanDNA-b6587/protein.faa -g 11 -q > /dev/null
[2023-03-18 23:15:38,517] [INFO] Task succeeded: Prodigal
[2023-03-18 23:15:38,517] [INFO] Task started: HMMsearch
[2023-03-18 23:15:38,517] [INFO] Running command: hmmsearch --tblout OceanDNA-b6587/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/reference_markers.hmm OceanDNA-b6587/protein.faa > /dev/null
[2023-03-18 23:15:38,769] [INFO] Task succeeded: HMMsearch
[2023-03-18 23:15:38,769] [INFO] Found 6/6 markers.
[2023-03-18 23:15:38,783] [INFO] Query marker FASTA was written to OceanDNA-b6587/markers.fasta
[2023-03-18 23:15:38,784] [INFO] Task started: Blastn
[2023-03-18 23:15:38,784] [INFO] Running command: blastn -query OceanDNA-b6587/markers.fasta -db /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/reference_markers.fasta -out OceanDNA-b6587/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:15:40,763] [INFO] Task succeeded: Blastn
[2023-03-18 23:15:40,765] [INFO] Selected 35 target genomes.
[2023-03-18 23:15:40,765] [INFO] Target genome list was writen to OceanDNA-b6587/target_genomes.txt
[2023-03-18 23:15:40,813] [INFO] Task started: fastANI
[2023-03-18 23:15:40,813] [INFO] Running command: fastANI --query /var/lib/cwl/stgf9f40036-9821-4105-8e9d-e0e215d632e9/OceanDNA-b6587.fa --refList OceanDNA-b6587/target_genomes.txt --output OceanDNA-b6587/fastani_result.tsv --threads 1
[2023-03-18 23:15:58,765] [INFO] Task succeeded: fastANI
[2023-03-18 23:15:58,765] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 23:15:58,765] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 23:15:58,765] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 23:15:58,766] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 23:15:58,766] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 23:15:58,766] [INFO] DFAST Taxonomy check result was written to OceanDNA-b6587/tc_result.tsv
[2023-03-18 23:15:58,766] [INFO] ===== Taxonomy check completed =====
[2023-03-18 23:15:58,766] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 23:15:58,766] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/checkm_data
[2023-03-18 23:15:58,769] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 23:15:58,772] [INFO] Task started: CheckM
[2023-03-18 23:15:58,772] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b6587/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b6587/checkm_input OceanDNA-b6587/checkm_result
[2023-03-18 23:16:36,449] [INFO] Task succeeded: CheckM
[2023-03-18 23:16:36,449] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 78.01%
Contamintation: 7.01%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 23:16:36,452] [INFO] ===== Completeness check finished =====
[2023-03-18 23:16:36,452] [INFO] ===== Start GTDB Search =====
[2023-03-18 23:16:36,452] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b6587/markers.fasta)
[2023-03-18 23:16:36,454] [INFO] Task started: Blastn
[2023-03-18 23:16:36,454] [INFO] Running command: blastn -query OceanDNA-b6587/markers.fasta -db /var/lib/cwl/stg942f9e35-a853-4943-b59b-29d9971fb23e/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b6587/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:16:37,360] [INFO] Task succeeded: Blastn
[2023-03-18 23:16:37,361] [INFO] Selected 29 target genomes.
[2023-03-18 23:16:37,361] [INFO] Target genome list was writen to OceanDNA-b6587/target_genomes_gtdb.txt
[2023-03-18 23:16:37,414] [INFO] Task started: fastANI
[2023-03-18 23:16:37,415] [INFO] Running command: fastANI --query /var/lib/cwl/stgf9f40036-9821-4105-8e9d-e0e215d632e9/OceanDNA-b6587.fa --refList OceanDNA-b6587/target_genomes_gtdb.txt --output OceanDNA-b6587/fastani_result_gtdb.tsv --threads 1
[2023-03-18 23:16:51,987] [INFO] Task succeeded: fastANI
[2023-03-18 23:16:51,991] [INFO] Found 5 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 23:16:51,991] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_011525065.1	s__BACL21 sp011525065	97.8209	303	501	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__BACL21	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_900299425.1	s__BACL21 sp900299425	78.4949	156	501	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__BACL21	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009921505.1	s__BACL21 sp009921505	78.1101	147	501	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__BACL21	95.0	97.10	97.10	0.82	0.82	2	-
GCA_002335345.1	s__BACL21 sp002335345	77.4836	137	501	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__BACL21	95.0	98.72	98.64	0.77	0.75	3	-
GCA_002384055.1	s__BACL21 sp002384055	76.9727	56	501	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__BACL21	95.0	99.79	99.68	0.93	0.91	4	-
--------------------------------------------------------------------------------
[2023-03-18 23:16:51,996] [INFO] GTDB search result was written to OceanDNA-b6587/result_gtdb.tsv
[2023-03-18 23:16:51,999] [INFO] ===== GTDB Search completed =====
[2023-03-18 23:16:51,999] [INFO] DFAST_QC result json was written to OceanDNA-b6587/dqc_result.json
[2023-03-18 23:16:51,999] [INFO] DFAST_QC completed!
[2023-03-18 23:16:51,999] [INFO] Total running time: 0h1m30s