[2023-03-15 08:09:45,414] [INFO] DFAST_QC pipeline started.
[2023-03-15 08:09:45,414] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 08:09:45,414] [INFO] DQC Reference Directory: /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference
[2023-03-15 08:09:46,759] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 08:09:46,760] [INFO] Task started: Prodigal
[2023-03-15 08:09:46,760] [INFO] Running command: cat /var/lib/cwl/stg06dcfed9-14b4-47d3-acb2-779281f0d3df/OceanDNA-b8214.fa | prodigal -d OceanDNA-b8214/cds.fna -a OceanDNA-b8214/protein.faa -g 11 -q > /dev/null
[2023-03-15 08:09:52,054] [INFO] Task succeeded: Prodigal
[2023-03-15 08:09:52,054] [INFO] Task started: HMMsearch
[2023-03-15 08:09:52,054] [INFO] Running command: hmmsearch --tblout OceanDNA-b8214/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/reference_markers.hmm OceanDNA-b8214/protein.faa > /dev/null
[2023-03-15 08:09:52,274] [INFO] Task succeeded: HMMsearch
[2023-03-15 08:09:52,275] [INFO] Found 6/6 markers.
[2023-03-15 08:09:52,283] [INFO] Query marker FASTA was written to OceanDNA-b8214/markers.fasta
[2023-03-15 08:09:52,284] [INFO] Task started: Blastn
[2023-03-15 08:09:52,284] [INFO] Running command: blastn -query OceanDNA-b8214/markers.fasta -db /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/reference_markers.fasta -out OceanDNA-b8214/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:09:52,892] [INFO] Task succeeded: Blastn
[2023-03-15 08:09:52,892] [INFO] Selected 33 target genomes.
[2023-03-15 08:09:52,893] [INFO] Target genome list was writen to OceanDNA-b8214/target_genomes.txt
[2023-03-15 08:09:52,908] [INFO] Task started: fastANI
[2023-03-15 08:09:52,908] [INFO] Running command: fastANI --query /var/lib/cwl/stg06dcfed9-14b4-47d3-acb2-779281f0d3df/OceanDNA-b8214.fa --refList OceanDNA-b8214/target_genomes.txt --output OceanDNA-b8214/fastani_result.tsv --threads 1
[2023-03-15 08:10:13,114] [INFO] Task succeeded: fastANI
[2023-03-15 08:10:13,115] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 08:10:13,115] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 08:10:13,115] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 08:10:13,115] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 08:10:13,115] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 08:10:13,115] [INFO] DFAST Taxonomy check result was written to OceanDNA-b8214/tc_result.tsv
[2023-03-15 08:10:13,116] [INFO] ===== Taxonomy check completed =====
[2023-03-15 08:10:13,116] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 08:10:13,116] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/checkm_data
[2023-03-15 08:10:13,119] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 08:10:13,122] [INFO] Task started: CheckM
[2023-03-15 08:10:13,122] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b8214/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b8214/checkm_input OceanDNA-b8214/checkm_result
[2023-03-15 08:10:32,937] [INFO] Task succeeded: CheckM
[2023-03-15 08:10:32,938] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 64.77%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 08:10:32,942] [INFO] ===== Completeness check finished =====
[2023-03-15 08:10:32,942] [INFO] ===== Start GTDB Search =====
[2023-03-15 08:10:32,942] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b8214/markers.fasta)
[2023-03-15 08:10:32,943] [INFO] Task started: Blastn
[2023-03-15 08:10:32,943] [INFO] Running command: blastn -query OceanDNA-b8214/markers.fasta -db /var/lib/cwl/stgb4a5d6fb-3e88-49bc-988e-42f6d1f128b8/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b8214/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:10:33,871] [INFO] Task succeeded: Blastn
[2023-03-15 08:10:33,873] [INFO] Selected 8 target genomes.
[2023-03-15 08:10:33,873] [INFO] Target genome list was writen to OceanDNA-b8214/target_genomes_gtdb.txt
[2023-03-15 08:10:33,880] [INFO] Task started: fastANI
[2023-03-15 08:10:33,880] [INFO] Running command: fastANI --query /var/lib/cwl/stg06dcfed9-14b4-47d3-acb2-779281f0d3df/OceanDNA-b8214.fa --refList OceanDNA-b8214/target_genomes_gtdb.txt --output OceanDNA-b8214/fastani_result_gtdb.tsv --threads 1
[2023-03-15 08:10:37,079] [INFO] Task succeeded: fastANI
[2023-03-15 08:10:37,084] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 08:10:37,084] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003331265.1	s__MAG-121220-bin8 sp003331265	81.4619	200	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905181705.1	s__MAG-121220-bin8 sp905181705	81.2029	227	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014239275.1	s__MAG-121220-bin8 sp014239275	79.39	183	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	99.08	98.67	0.74	0.67	4	-
GCA_902511755.1	s__MAG-121220-bin8 sp902511755	79.2173	145	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	99.22	98.71	0.74	0.72	3	-
GCA_002702895.1	s__MAG-121220-bin8 sp002702895	79.1646	188	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	N/A	N/A	N/A	N/A	1	-
GCA_012959395.1	s__MAG-121220-bin8 sp012959395	78.3655	135	289	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__MAG-121220-bin8	95.0	97.96	97.81	0.81	0.77	13	-
--------------------------------------------------------------------------------
[2023-03-15 08:10:37,085] [INFO] GTDB search result was written to OceanDNA-b8214/result_gtdb.tsv
[2023-03-15 08:10:37,085] [INFO] ===== GTDB Search completed =====
[2023-03-15 08:10:37,086] [INFO] DFAST_QC result json was written to OceanDNA-b8214/dqc_result.json
[2023-03-15 08:10:37,086] [INFO] DFAST_QC completed!
[2023-03-15 08:10:37,086] [INFO] Total running time: 0h0m52s