[2023-06-05 05:15:25,591] [INFO] DFAST_QC pipeline started.
[2023-06-05 05:15:25,593] [INFO] DFAST_QC version: 0.5.7
[2023-06-05 05:15:25,594] [INFO] DQC Reference Directory: /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference
[2023-06-05 05:15:27,188] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-05 05:15:27,189] [INFO] Task started: Prodigal
[2023-06-05 05:15:27,189] [INFO] Running command: gunzip -c /var/lib/cwl/stge0d883e5-72b4-4b10-8895-ac2d8d564ce9/GCA_001776335.1_ASM177633v1_genomic.fna.gz | prodigal -d GCA_001776335.1_ASM177633v1_genomic.fna/cds.fna -a GCA_001776335.1_ASM177633v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-05 05:15:30,479] [INFO] Task succeeded: Prodigal
[2023-06-05 05:15:30,480] [INFO] Task started: HMMsearch
[2023-06-05 05:15:30,480] [INFO] Running command: hmmsearch --tblout GCA_001776335.1_ASM177633v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/reference_markers.hmm GCA_001776335.1_ASM177633v1_genomic.fna/protein.faa > /dev/null
[2023-06-05 05:15:30,719] [INFO] Task succeeded: HMMsearch
[2023-06-05 05:15:30,720] [WARNING] Found 5/6 markers. [/var/lib/cwl/stge0d883e5-72b4-4b10-8895-ac2d8d564ce9/GCA_001776335.1_ASM177633v1_genomic.fna.gz]
[2023-06-05 05:15:30,737] [INFO] Query marker FASTA was written to GCA_001776335.1_ASM177633v1_genomic.fna/markers.fasta
[2023-06-05 05:15:30,737] [INFO] Task started: Blastn
[2023-06-05 05:15:30,738] [INFO] Running command: blastn -query GCA_001776335.1_ASM177633v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/reference_markers.fasta -out GCA_001776335.1_ASM177633v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 05:15:31,308] [INFO] Task succeeded: Blastn
[2023-06-05 05:15:31,312] [INFO] Selected 14 target genomes.
[2023-06-05 05:15:31,312] [INFO] Target genome list was writen to GCA_001776335.1_ASM177633v1_genomic.fna/target_genomes.txt
[2023-06-05 05:15:31,316] [INFO] Task started: fastANI
[2023-06-05 05:15:31,317] [INFO] Running command: fastANI --query /var/lib/cwl/stge0d883e5-72b4-4b10-8895-ac2d8d564ce9/GCA_001776335.1_ASM177633v1_genomic.fna.gz --refList GCA_001776335.1_ASM177633v1_genomic.fna/target_genomes.txt --output GCA_001776335.1_ASM177633v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-05 05:15:38,522] [INFO] Task succeeded: fastANI
[2023-06-05 05:15:38,522] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-05 05:15:38,523] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-05 05:15:38,525] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-05 05:15:38,525] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-05 05:15:38,526] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-05 05:15:38,528] [INFO] DFAST Taxonomy check result was written to GCA_001776335.1_ASM177633v1_genomic.fna/tc_result.tsv
[2023-06-05 05:15:38,529] [INFO] ===== Taxonomy check completed =====
[2023-06-05 05:15:38,529] [INFO] ===== Start completeness check using CheckM =====
[2023-06-05 05:15:38,529] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/checkm_data
[2023-06-05 05:15:38,532] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-05 05:15:38,547] [INFO] Task started: CheckM
[2023-06-05 05:15:38,547] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_001776335.1_ASM177633v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_001776335.1_ASM177633v1_genomic.fna/checkm_input GCA_001776335.1_ASM177633v1_genomic.fna/checkm_result
[2023-06-05 05:15:56,324] [INFO] Task succeeded: CheckM
[2023-06-05 05:15:56,325] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.04%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-05 05:15:56,343] [INFO] ===== Completeness check finished =====
[2023-06-05 05:15:56,344] [INFO] ===== Start GTDB Search =====
[2023-06-05 05:15:56,344] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_001776335.1_ASM177633v1_genomic.fna/markers.fasta)
[2023-06-05 05:15:56,344] [INFO] Task started: Blastn
[2023-06-05 05:15:56,344] [INFO] Running command: blastn -query GCA_001776335.1_ASM177633v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3d659fa2-98cb-4e43-8f4c-fc8f1a93f748/dqc_reference/reference_markers_gtdb.fasta -out GCA_001776335.1_ASM177633v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 05:15:57,095] [INFO] Task succeeded: Blastn
[2023-06-05 05:15:57,099] [INFO] Selected 17 target genomes.
[2023-06-05 05:15:57,100] [INFO] Target genome list was writen to GCA_001776335.1_ASM177633v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-05 05:15:57,269] [INFO] Task started: fastANI
[2023-06-05 05:15:57,270] [INFO] Running command: fastANI --query /var/lib/cwl/stge0d883e5-72b4-4b10-8895-ac2d8d564ce9/GCA_001776335.1_ASM177633v1_genomic.fna.gz --refList GCA_001776335.1_ASM177633v1_genomic.fna/target_genomes_gtdb.txt --output GCA_001776335.1_ASM177633v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-05 05:16:02,226] [INFO] Task succeeded: fastANI
[2023-06-05 05:16:02,229] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-05 05:16:02,229] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001776335.1	s__2-01-FULL-41-11 sp001776335	100.0	248	253	d__Bacteria;p__Patescibacteria;c__Microgenomatia;o__Curtissbacterales;f__GWA2-41-24;g__2-01-FULL-41-11	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-05 05:16:02,231] [INFO] GTDB search result was written to GCA_001776335.1_ASM177633v1_genomic.fna/result_gtdb.tsv
[2023-06-05 05:16:02,231] [INFO] ===== GTDB Search completed =====
[2023-06-05 05:16:02,234] [INFO] DFAST_QC result json was written to GCA_001776335.1_ASM177633v1_genomic.fna/dqc_result.json
[2023-06-05 05:16:02,234] [INFO] DFAST_QC completed!
[2023-06-05 05:16:02,234] [INFO] Total running time: 0h0m37s