[2023-06-28 00:19:20,808] [INFO] DFAST_QC pipeline started.
[2023-06-28 00:19:20,810] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 00:19:20,811] [INFO] DQC Reference Directory: /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference
[2023-06-28 00:19:22,058] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 00:19:22,059] [INFO] Task started: Prodigal
[2023-06-28 00:19:22,059] [INFO] Running command: gunzip -c /var/lib/cwl/stg2bdc0acd-8379-42a2-aae9-2df4c66c3968/GCA_002084345.1_ASM208434v1_genomic.fna.gz | prodigal -d GCA_002084345.1_ASM208434v1_genomic.fna/cds.fna -a GCA_002084345.1_ASM208434v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 00:19:28,312] [INFO] Task succeeded: Prodigal
[2023-06-28 00:19:28,312] [INFO] Task started: HMMsearch
[2023-06-28 00:19:28,313] [INFO] Running command: hmmsearch --tblout GCA_002084345.1_ASM208434v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/reference_markers.hmm GCA_002084345.1_ASM208434v1_genomic.fna/protein.faa > /dev/null
[2023-06-28 00:19:28,564] [INFO] Task succeeded: HMMsearch
[2023-06-28 00:19:28,565] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg2bdc0acd-8379-42a2-aae9-2df4c66c3968/GCA_002084345.1_ASM208434v1_genomic.fna.gz]
[2023-06-28 00:19:28,593] [INFO] Query marker FASTA was written to GCA_002084345.1_ASM208434v1_genomic.fna/markers.fasta
[2023-06-28 00:19:28,593] [INFO] Task started: Blastn
[2023-06-28 00:19:28,593] [INFO] Running command: blastn -query GCA_002084345.1_ASM208434v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/reference_markers.fasta -out GCA_002084345.1_ASM208434v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 00:19:29,090] [INFO] Task succeeded: Blastn
[2023-06-28 00:19:29,095] [INFO] Selected 5 target genomes.
[2023-06-28 00:19:29,096] [INFO] Target genome list was writen to GCA_002084345.1_ASM208434v1_genomic.fna/target_genomes.txt
[2023-06-28 00:19:29,097] [INFO] Task started: fastANI
[2023-06-28 00:19:29,097] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bdc0acd-8379-42a2-aae9-2df4c66c3968/GCA_002084345.1_ASM208434v1_genomic.fna.gz --refList GCA_002084345.1_ASM208434v1_genomic.fna/target_genomes.txt --output GCA_002084345.1_ASM208434v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 00:19:32,241] [INFO] Task succeeded: fastANI
[2023-06-28 00:19:32,242] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 00:19:32,242] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 00:19:32,244] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-28 00:19:32,244] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-28 00:19:32,244] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-28 00:19:32,247] [INFO] DFAST Taxonomy check result was written to GCA_002084345.1_ASM208434v1_genomic.fna/tc_result.tsv
[2023-06-28 00:19:32,248] [INFO] ===== Taxonomy check completed =====
[2023-06-28 00:19:32,249] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 00:19:32,249] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/checkm_data
[2023-06-28 00:19:32,253] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 00:19:32,280] [INFO] Task started: CheckM
[2023-06-28 00:19:32,280] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_002084345.1_ASM208434v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_002084345.1_ASM208434v1_genomic.fna/checkm_input GCA_002084345.1_ASM208434v1_genomic.fna/checkm_result
[2023-06-28 00:19:57,383] [INFO] Task succeeded: CheckM
[2023-06-28 00:19:57,385] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.83%
Contamintation: 16.67%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-28 00:19:57,410] [INFO] ===== Completeness check finished =====
[2023-06-28 00:19:57,411] [INFO] ===== Start GTDB Search =====
[2023-06-28 00:19:57,411] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_002084345.1_ASM208434v1_genomic.fna/markers.fasta)
[2023-06-28 00:19:57,411] [INFO] Task started: Blastn
[2023-06-28 00:19:57,411] [INFO] Running command: blastn -query GCA_002084345.1_ASM208434v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb480a001-e14e-41be-9ec7-109ed55082f3/dqc_reference/reference_markers_gtdb.fasta -out GCA_002084345.1_ASM208434v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 00:19:57,893] [INFO] Task succeeded: Blastn
[2023-06-28 00:19:57,897] [INFO] Selected 11 target genomes.
[2023-06-28 00:19:57,897] [INFO] Target genome list was writen to GCA_002084345.1_ASM208434v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 00:19:57,904] [INFO] Task started: fastANI
[2023-06-28 00:19:57,904] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bdc0acd-8379-42a2-aae9-2df4c66c3968/GCA_002084345.1_ASM208434v1_genomic.fna.gz --refList GCA_002084345.1_ASM208434v1_genomic.fna/target_genomes_gtdb.txt --output GCA_002084345.1_ASM208434v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 00:20:04,065] [INFO] Task succeeded: fastANI
[2023-06-28 00:20:04,070] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-28 00:20:04,070] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_016931785.1	s__JAFGED01 sp016931785	76.4413	60	672	d__Bacteria;p__Chloroflexota;c__Anaerolineae;o__B4-G1;f__B4-G1;g__JAFGED01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-28 00:20:04,072] [INFO] GTDB search result was written to GCA_002084345.1_ASM208434v1_genomic.fna/result_gtdb.tsv
[2023-06-28 00:20:04,073] [INFO] ===== GTDB Search completed =====
[2023-06-28 00:20:04,076] [INFO] DFAST_QC result json was written to GCA_002084345.1_ASM208434v1_genomic.fna/dqc_result.json
[2023-06-28 00:20:04,077] [INFO] DFAST_QC completed!
[2023-06-28 00:20:04,077] [INFO] Total running time: 0h0m43s