[2023-06-26 20:47:25,985] [INFO] DFAST_QC pipeline started.
[2023-06-26 20:47:26,005] [INFO] DFAST_QC version: 0.5.7
[2023-06-26 20:47:26,006] [INFO] DQC Reference Directory: /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference
[2023-06-26 20:47:27,521] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-26 20:47:27,527] [INFO] Task started: Prodigal
[2023-06-26 20:47:27,527] [INFO] Running command: gunzip -c /var/lib/cwl/stge94b75ca-7dbd-46e8-a697-8f958c3694c7/GCA_002433595.1_ASM243359v1_genomic.fna.gz | prodigal -d GCA_002433595.1_ASM243359v1_genomic.fna/cds.fna -a GCA_002433595.1_ASM243359v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-26 20:47:30,623] [INFO] Task succeeded: Prodigal
[2023-06-26 20:47:30,624] [INFO] Task started: HMMsearch
[2023-06-26 20:47:30,624] [INFO] Running command: hmmsearch --tblout GCA_002433595.1_ASM243359v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/reference_markers.hmm GCA_002433595.1_ASM243359v1_genomic.fna/protein.faa > /dev/null
[2023-06-26 20:47:30,829] [INFO] Task succeeded: HMMsearch
[2023-06-26 20:47:30,830] [INFO] Found 6/6 markers.
[2023-06-26 20:47:30,861] [INFO] Query marker FASTA was written to GCA_002433595.1_ASM243359v1_genomic.fna/markers.fasta
[2023-06-26 20:47:30,861] [INFO] Task started: Blastn
[2023-06-26 20:47:30,862] [INFO] Running command: blastn -query GCA_002433595.1_ASM243359v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/reference_markers.fasta -out GCA_002433595.1_ASM243359v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-26 20:47:31,515] [INFO] Task succeeded: Blastn
[2023-06-26 20:47:31,533] [INFO] Selected 14 target genomes.
[2023-06-26 20:47:31,534] [INFO] Target genome list was writen to GCA_002433595.1_ASM243359v1_genomic.fna/target_genomes.txt
[2023-06-26 20:47:31,538] [INFO] Task started: fastANI
[2023-06-26 20:47:31,539] [INFO] Running command: fastANI --query /var/lib/cwl/stge94b75ca-7dbd-46e8-a697-8f958c3694c7/GCA_002433595.1_ASM243359v1_genomic.fna.gz --refList GCA_002433595.1_ASM243359v1_genomic.fna/target_genomes.txt --output GCA_002433595.1_ASM243359v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-26 20:47:41,674] [INFO] Task succeeded: fastANI
[2023-06-26 20:47:41,674] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-26 20:47:41,674] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-26 20:47:41,677] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-26 20:47:41,677] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-26 20:47:41,677] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-26 20:47:41,688] [INFO] DFAST Taxonomy check result was written to GCA_002433595.1_ASM243359v1_genomic.fna/tc_result.tsv
[2023-06-26 20:47:41,689] [INFO] ===== Taxonomy check completed =====
[2023-06-26 20:47:41,689] [INFO] ===== Start completeness check using CheckM =====
[2023-06-26 20:47:41,690] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/checkm_data
[2023-06-26 20:47:41,694] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-26 20:47:41,716] [INFO] Task started: CheckM
[2023-06-26 20:47:41,717] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_002433595.1_ASM243359v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_002433595.1_ASM243359v1_genomic.fna/checkm_input GCA_002433595.1_ASM243359v1_genomic.fna/checkm_result
[2023-06-26 20:47:59,321] [INFO] Task succeeded: CheckM
[2023-06-26 20:47:59,323] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.52%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-26 20:47:59,348] [INFO] ===== Completeness check finished =====
[2023-06-26 20:47:59,348] [INFO] ===== Start GTDB Search =====
[2023-06-26 20:47:59,349] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_002433595.1_ASM243359v1_genomic.fna/markers.fasta)
[2023-06-26 20:47:59,349] [INFO] Task started: Blastn
[2023-06-26 20:47:59,349] [INFO] Running command: blastn -query GCA_002433595.1_ASM243359v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg08cb48c3-245d-401f-aee6-73df9728e029/dqc_reference/reference_markers_gtdb.fasta -out GCA_002433595.1_ASM243359v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-26 20:48:00,198] [INFO] Task succeeded: Blastn
[2023-06-26 20:48:00,202] [INFO] Selected 14 target genomes.
[2023-06-26 20:48:00,202] [INFO] Target genome list was writen to GCA_002433595.1_ASM243359v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-26 20:48:00,280] [INFO] Task started: fastANI
[2023-06-26 20:48:00,280] [INFO] Running command: fastANI --query /var/lib/cwl/stge94b75ca-7dbd-46e8-a697-8f958c3694c7/GCA_002433595.1_ASM243359v1_genomic.fna.gz --refList GCA_002433595.1_ASM243359v1_genomic.fna/target_genomes_gtdb.txt --output GCA_002433595.1_ASM243359v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-26 20:48:05,566] [INFO] Task succeeded: fastANI
[2023-06-26 20:48:05,575] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-26 20:48:05,575] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002433595.1	s__UBA6595 sp002433595	100.0	345	351	d__Bacteria;p__Margulisbacteria;c__Marinamargulisbacteria;o__UBA6595;f__UBA6595;g__UBA6595	95.0	99.81	99.79	0.90	0.88	3	conclusive
--------------------------------------------------------------------------------
[2023-06-26 20:48:05,578] [INFO] GTDB search result was written to GCA_002433595.1_ASM243359v1_genomic.fna/result_gtdb.tsv
[2023-06-26 20:48:05,579] [INFO] ===== GTDB Search completed =====
[2023-06-26 20:48:05,595] [INFO] DFAST_QC result json was written to GCA_002433595.1_ASM243359v1_genomic.fna/dqc_result.json
[2023-06-26 20:48:05,595] [INFO] DFAST_QC completed!
[2023-06-26 20:48:05,596] [INFO] Total running time: 0h0m40s