[2023-06-16 19:07:00,321] [INFO] DFAST_QC pipeline started.
[2023-06-16 19:07:00,324] [INFO] DFAST_QC version: 0.5.7
[2023-06-16 19:07:00,324] [INFO] DQC Reference Directory: /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference
[2023-06-16 19:07:01,570] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-16 19:07:01,571] [INFO] Task started: Prodigal
[2023-06-16 19:07:01,571] [INFO] Running command: gunzip -c /var/lib/cwl/stgf604fa0d-acd1-454f-9f69-5c94fb8a2a6f/GCA_003246545.1_ASM324654v1_genomic.fna.gz | prodigal -d GCA_003246545.1_ASM324654v1_genomic.fna/cds.fna -a GCA_003246545.1_ASM324654v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-16 19:07:02,723] [INFO] Task succeeded: Prodigal
[2023-06-16 19:07:02,723] [INFO] Task started: HMMsearch
[2023-06-16 19:07:02,723] [INFO] Running command: hmmsearch --tblout GCA_003246545.1_ASM324654v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/reference_markers.hmm GCA_003246545.1_ASM324654v1_genomic.fna/protein.faa > /dev/null
[2023-06-16 19:07:02,818] [INFO] Task succeeded: HMMsearch
[2023-06-16 19:07:02,819] [WARNING] Found 1/6 markers. [/var/lib/cwl/stgf604fa0d-acd1-454f-9f69-5c94fb8a2a6f/GCA_003246545.1_ASM324654v1_genomic.fna.gz]
[2023-06-16 19:07:02,834] [INFO] Query marker FASTA was written to GCA_003246545.1_ASM324654v1_genomic.fna/markers.fasta
[2023-06-16 19:07:02,834] [INFO] Task started: Blastn
[2023-06-16 19:07:02,834] [INFO] Running command: blastn -query GCA_003246545.1_ASM324654v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/reference_markers.fasta -out GCA_003246545.1_ASM324654v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-16 19:07:03,313] [INFO] Task succeeded: Blastn
[2023-06-16 19:07:03,317] [INFO] Selected 8 target genomes.
[2023-06-16 19:07:03,317] [INFO] Target genome list was writen to GCA_003246545.1_ASM324654v1_genomic.fna/target_genomes.txt
[2023-06-16 19:07:03,321] [INFO] Task started: fastANI
[2023-06-16 19:07:03,321] [INFO] Running command: fastANI --query /var/lib/cwl/stgf604fa0d-acd1-454f-9f69-5c94fb8a2a6f/GCA_003246545.1_ASM324654v1_genomic.fna.gz --refList GCA_003246545.1_ASM324654v1_genomic.fna/target_genomes.txt --output GCA_003246545.1_ASM324654v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-16 19:07:07,512] [INFO] Task succeeded: fastANI
[2023-06-16 19:07:07,513] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-16 19:07:07,513] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-16 19:07:07,515] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-16 19:07:07,515] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-16 19:07:07,515] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-16 19:07:07,517] [INFO] DFAST Taxonomy check result was written to GCA_003246545.1_ASM324654v1_genomic.fna/tc_result.tsv
[2023-06-16 19:07:07,517] [INFO] ===== Taxonomy check completed =====
[2023-06-16 19:07:07,517] [INFO] ===== Start completeness check using CheckM =====
[2023-06-16 19:07:07,518] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/checkm_data
[2023-06-16 19:07:07,520] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-16 19:07:07,529] [INFO] Task started: CheckM
[2023-06-16 19:07:07,529] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_003246545.1_ASM324654v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_003246545.1_ASM324654v1_genomic.fna/checkm_input GCA_003246545.1_ASM324654v1_genomic.fna/checkm_result
[2023-06-16 19:07:18,280] [INFO] Task succeeded: CheckM
[2023-06-16 19:07:18,282] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 39.58%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-16 19:07:18,299] [INFO] ===== Completeness check finished =====
[2023-06-16 19:07:18,299] [INFO] ===== Start GTDB Search =====
[2023-06-16 19:07:18,299] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_003246545.1_ASM324654v1_genomic.fna/markers.fasta)
[2023-06-16 19:07:18,300] [INFO] Task started: Blastn
[2023-06-16 19:07:18,300] [INFO] Running command: blastn -query GCA_003246545.1_ASM324654v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb2ece469-5df2-4f61-8e68-a2b28f496f62/dqc_reference/reference_markers_gtdb.fasta -out GCA_003246545.1_ASM324654v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-16 19:07:18,797] [INFO] Task succeeded: Blastn
[2023-06-16 19:07:18,801] [INFO] Selected 8 target genomes.
[2023-06-16 19:07:18,801] [INFO] Target genome list was writen to GCA_003246545.1_ASM324654v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-16 19:07:18,810] [INFO] Task started: fastANI
[2023-06-16 19:07:18,810] [INFO] Running command: fastANI --query /var/lib/cwl/stgf604fa0d-acd1-454f-9f69-5c94fb8a2a6f/GCA_003246545.1_ASM324654v1_genomic.fna.gz --refList GCA_003246545.1_ASM324654v1_genomic.fna/target_genomes_gtdb.txt --output GCA_003246545.1_ASM324654v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-16 19:07:24,541] [INFO] Task succeeded: fastANI
[2023-06-16 19:07:24,544] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-16 19:07:24,545] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003249625.1	s__Propionivibrio sp003249625	83.8115	74	118	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Rhodocyclaceae;g__Propionivibrio	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-16 19:07:24,547] [INFO] GTDB search result was written to GCA_003246545.1_ASM324654v1_genomic.fna/result_gtdb.tsv
[2023-06-16 19:07:24,547] [INFO] ===== GTDB Search completed =====
[2023-06-16 19:07:24,549] [INFO] DFAST_QC result json was written to GCA_003246545.1_ASM324654v1_genomic.fna/dqc_result.json
[2023-06-16 19:07:24,550] [INFO] DFAST_QC completed!
[2023-06-16 19:07:24,550] [INFO] Total running time: 0h0m24s