[2023-06-05 02:23:13,330] [INFO] DFAST_QC pipeline started.
[2023-06-05 02:23:13,332] [INFO] DFAST_QC version: 0.5.7
[2023-06-05 02:23:13,332] [INFO] DQC Reference Directory: /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference
[2023-06-05 02:23:17,171] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-05 02:23:17,173] [INFO] Task started: Prodigal
[2023-06-05 02:23:17,173] [INFO] Running command: gunzip -c /var/lib/cwl/stg94bb8cd3-d41f-4256-ba44-b23c4f74320b/GCA_004551895.1_ASM455189v1_genomic.fna.gz | prodigal -d GCA_004551895.1_ASM455189v1_genomic.fna/cds.fna -a GCA_004551895.1_ASM455189v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-05 02:23:21,920] [INFO] Task succeeded: Prodigal
[2023-06-05 02:23:21,921] [INFO] Task started: HMMsearch
[2023-06-05 02:23:21,921] [INFO] Running command: hmmsearch --tblout GCA_004551895.1_ASM455189v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/reference_markers.hmm GCA_004551895.1_ASM455189v1_genomic.fna/protein.faa > /dev/null
[2023-06-05 02:23:22,130] [INFO] Task succeeded: HMMsearch
[2023-06-05 02:23:22,132] [INFO] Found 6/6 markers.
[2023-06-05 02:23:22,159] [INFO] Query marker FASTA was written to GCA_004551895.1_ASM455189v1_genomic.fna/markers.fasta
[2023-06-05 02:23:22,160] [INFO] Task started: Blastn
[2023-06-05 02:23:22,160] [INFO] Running command: blastn -query GCA_004551895.1_ASM455189v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/reference_markers.fasta -out GCA_004551895.1_ASM455189v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 02:23:23,979] [INFO] Task succeeded: Blastn
[2023-06-05 02:23:23,985] [INFO] Selected 26 target genomes.
[2023-06-05 02:23:23,986] [INFO] Target genome list was writen to GCA_004551895.1_ASM455189v1_genomic.fna/target_genomes.txt
[2023-06-05 02:23:23,989] [INFO] Task started: fastANI
[2023-06-05 02:23:23,989] [INFO] Running command: fastANI --query /var/lib/cwl/stg94bb8cd3-d41f-4256-ba44-b23c4f74320b/GCA_004551895.1_ASM455189v1_genomic.fna.gz --refList GCA_004551895.1_ASM455189v1_genomic.fna/target_genomes.txt --output GCA_004551895.1_ASM455189v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-05 02:23:36,894] [INFO] Task succeeded: fastANI
[2023-06-05 02:23:36,895] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-05 02:23:36,895] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-05 02:23:36,905] [INFO] Found 10 fastANI hits (0 hits with ANI > threshold)
[2023-06-05 02:23:36,905] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-06-05 02:23:36,906] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Dysosmobacter acutus	strain=MSJ-2	GCA_018919205.1	2841504	2841504	type	True	77.472	63	501	95	below_threshold
Flavonifractor plautii	strain=ATCC 29863	GCA_000239295.1	292800	292800	suspected-type	True	77.4121	61	501	95	below_threshold
Flavonifractor plautii	strain=JCM 32125	GCA_010508875.1	292800	292800	suspected-type	True	77.2814	65	501	95	below_threshold
Pseudoflavonifractor gallinarum	strain=DSM 107456	GCA_014982855.1	2779352	2779352	type	True	77.1988	64	501	95	below_threshold
Vescimonas fastidiosa	strain=MM35	GCA_018326305.1	2714353	2714353	type	True	76.9963	56	501	95	below_threshold
Dysosmobacter welbionis	strain=J115	GCA_005121165.3	2093857	2093857	type	True	76.8825	61	501	95	below_threshold
Intestinimonas butyriciproducens	strain=DSM 26588	GCA_003096335.1	1297617	1297617	type	True	76.866	51	501	95	below_threshold
Clostridium phoceensis	strain=GD3	GCA_001244495.1	1650661	1650661	type	True	76.8643	52	501	95	below_threshold
Intestinimonas butyriciproducens	strain=DSM 26588	GCA_024622565.1	1297617	1297617	type	True	76.8202	52	501	95	below_threshold
Anaerotruncus massiliensis	strain=AT3	GCA_900199635.1	1673720	1673720	type	True	76.7249	67	501	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-05 02:23:36,908] [INFO] DFAST Taxonomy check result was written to GCA_004551895.1_ASM455189v1_genomic.fna/tc_result.tsv
[2023-06-05 02:23:36,908] [INFO] ===== Taxonomy check completed =====
[2023-06-05 02:23:36,909] [INFO] ===== Start completeness check using CheckM =====
[2023-06-05 02:23:36,909] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/checkm_data
[2023-06-05 02:23:36,910] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-05 02:23:36,933] [INFO] Task started: CheckM
[2023-06-05 02:23:36,934] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_004551895.1_ASM455189v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_004551895.1_ASM455189v1_genomic.fna/checkm_input GCA_004551895.1_ASM455189v1_genomic.fna/checkm_result
[2023-06-05 02:23:57,065] [INFO] Task succeeded: CheckM
[2023-06-05 02:23:57,067] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-05 02:23:57,086] [INFO] ===== Completeness check finished =====
[2023-06-05 02:23:57,086] [INFO] ===== Start GTDB Search =====
[2023-06-05 02:23:57,086] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_004551895.1_ASM455189v1_genomic.fna/markers.fasta)
[2023-06-05 02:23:57,087] [INFO] Task started: Blastn
[2023-06-05 02:23:57,087] [INFO] Running command: blastn -query GCA_004551895.1_ASM455189v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9dc8a4ee-dc97-4737-896f-db4fea2c5cb9/dqc_reference/reference_markers_gtdb.fasta -out GCA_004551895.1_ASM455189v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 02:23:58,471] [INFO] Task succeeded: Blastn
[2023-06-05 02:23:58,479] [INFO] Selected 6 target genomes.
[2023-06-05 02:23:58,480] [INFO] Target genome list was writen to GCA_004551895.1_ASM455189v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-05 02:23:58,480] [INFO] Task started: fastANI
[2023-06-05 02:23:58,480] [INFO] Running command: fastANI --query /var/lib/cwl/stg94bb8cd3-d41f-4256-ba44-b23c4f74320b/GCA_004551895.1_ASM455189v1_genomic.fna.gz --refList GCA_004551895.1_ASM455189v1_genomic.fna/target_genomes_gtdb.txt --output GCA_004551895.1_ASM455189v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-05 02:24:00,883] [INFO] Task succeeded: fastANI
[2023-06-05 02:24:00,894] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-05 02:24:00,894] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900317855.1	s__CAG-103 sp900317855	95.8689	431	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	97.11	95.56	0.88	0.84	11	conclusive
GCA_000432375.1	s__CAG-103 sp000432375	94.1818	400	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	98.15	97.87	0.87	0.82	10	-
GCA_900757655.1	s__CAG-103 sp900757655	94.0611	407	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	96.74	95.66	0.88	0.82	7	-
GCA_900543625.1	s__CAG-103 sp900543625	90.59	426	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	96.93	95.23	0.89	0.87	12	-
GCA_004556965.1	s__CAG-103 sp004556965	82.0606	299	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	98.49	98.32	0.91	0.88	6	-
GCA_018265835.1	s__CAG-103 sp018265835	81.5551	292	501	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Oscillospiraceae;g__CAG-103	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-05 02:24:00,897] [INFO] GTDB search result was written to GCA_004551895.1_ASM455189v1_genomic.fna/result_gtdb.tsv
[2023-06-05 02:24:00,898] [INFO] ===== GTDB Search completed =====
[2023-06-05 02:24:00,903] [INFO] DFAST_QC result json was written to GCA_004551895.1_ASM455189v1_genomic.fna/dqc_result.json
[2023-06-05 02:24:00,903] [INFO] DFAST_QC completed!
[2023-06-05 02:24:00,904] [INFO] Total running time: 0h0m48s