[2023-06-04 20:01:53,487] [INFO] DFAST_QC pipeline started.
[2023-06-04 20:01:53,489] [INFO] DFAST_QC version: 0.5.7
[2023-06-04 20:01:53,489] [INFO] DQC Reference Directory: /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference
[2023-06-04 20:01:54,914] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-04 20:01:54,915] [INFO] Task started: Prodigal
[2023-06-04 20:01:54,916] [INFO] Running command: gunzip -c /var/lib/cwl/stgd4782405-e2f8-4467-a919-bfb9d9233028/GCA_004557935.1_ASM455793v1_genomic.fna.gz | prodigal -d GCA_004557935.1_ASM455793v1_genomic.fna/cds.fna -a GCA_004557935.1_ASM455793v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-04 20:01:57,192] [INFO] Task succeeded: Prodigal
[2023-06-04 20:01:57,192] [INFO] Task started: HMMsearch
[2023-06-04 20:01:57,192] [INFO] Running command: hmmsearch --tblout GCA_004557935.1_ASM455793v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/reference_markers.hmm GCA_004557935.1_ASM455793v1_genomic.fna/protein.faa > /dev/null
[2023-06-04 20:01:57,405] [INFO] Task succeeded: HMMsearch
[2023-06-04 20:01:57,406] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgd4782405-e2f8-4467-a919-bfb9d9233028/GCA_004557935.1_ASM455793v1_genomic.fna.gz]
[2023-06-04 20:01:57,425] [INFO] Query marker FASTA was written to GCA_004557935.1_ASM455793v1_genomic.fna/markers.fasta
[2023-06-04 20:01:57,425] [INFO] Task started: Blastn
[2023-06-04 20:01:57,425] [INFO] Running command: blastn -query GCA_004557935.1_ASM455793v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/reference_markers.fasta -out GCA_004557935.1_ASM455793v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-04 20:01:58,673] [INFO] Task succeeded: Blastn
[2023-06-04 20:01:58,678] [INFO] Selected 13 target genomes.
[2023-06-04 20:01:58,678] [INFO] Target genome list was writen to GCA_004557935.1_ASM455793v1_genomic.fna/target_genomes.txt
[2023-06-04 20:01:58,824] [INFO] Task started: fastANI
[2023-06-04 20:01:58,825] [INFO] Running command: fastANI --query /var/lib/cwl/stgd4782405-e2f8-4467-a919-bfb9d9233028/GCA_004557935.1_ASM455793v1_genomic.fna.gz --refList GCA_004557935.1_ASM455793v1_genomic.fna/target_genomes.txt --output GCA_004557935.1_ASM455793v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-04 20:02:05,944] [INFO] Task succeeded: fastANI
[2023-06-04 20:02:05,945] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-04 20:02:05,946] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-04 20:02:05,948] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-04 20:02:05,948] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-04 20:02:05,949] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-04 20:02:05,958] [INFO] DFAST Taxonomy check result was written to GCA_004557935.1_ASM455793v1_genomic.fna/tc_result.tsv
[2023-06-04 20:02:05,960] [INFO] ===== Taxonomy check completed =====
[2023-06-04 20:02:05,960] [INFO] ===== Start completeness check using CheckM =====
[2023-06-04 20:02:05,961] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/checkm_data
[2023-06-04 20:02:05,963] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-04 20:02:05,978] [INFO] Task started: CheckM
[2023-06-04 20:02:05,978] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_004557935.1_ASM455793v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_004557935.1_ASM455793v1_genomic.fna/checkm_input GCA_004557935.1_ASM455793v1_genomic.fna/checkm_result
[2023-06-04 20:02:20,996] [INFO] Task succeeded: CheckM
[2023-06-04 20:02:20,997] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 84.90%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-04 20:02:21,022] [INFO] ===== Completeness check finished =====
[2023-06-04 20:02:21,023] [INFO] ===== Start GTDB Search =====
[2023-06-04 20:02:21,023] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_004557935.1_ASM455793v1_genomic.fna/markers.fasta)
[2023-06-04 20:02:21,024] [INFO] Task started: Blastn
[2023-06-04 20:02:21,024] [INFO] Running command: blastn -query GCA_004557935.1_ASM455793v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg69445bf3-e129-44ad-a83d-820aa7ffba5b/dqc_reference/reference_markers_gtdb.fasta -out GCA_004557935.1_ASM455793v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-04 20:02:21,786] [INFO] Task succeeded: Blastn
[2023-06-04 20:02:21,791] [INFO] Selected 14 target genomes.
[2023-06-04 20:02:21,791] [INFO] Target genome list was writen to GCA_004557935.1_ASM455793v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-04 20:02:21,897] [INFO] Task started: fastANI
[2023-06-04 20:02:21,898] [INFO] Running command: fastANI --query /var/lib/cwl/stgd4782405-e2f8-4467-a919-bfb9d9233028/GCA_004557935.1_ASM455793v1_genomic.fna.gz --refList GCA_004557935.1_ASM455793v1_genomic.fna/target_genomes_gtdb.txt --output GCA_004557935.1_ASM455793v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-04 20:02:25,718] [INFO] Task succeeded: fastANI
[2023-06-04 20:02:25,723] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-04 20:02:25,724] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004558105.1	s__SFEB01 sp004558105	98.0643	231	264	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-314;g__SFEB01	95.0	97.80	97.16	0.85	0.80	9	conclusive
--------------------------------------------------------------------------------
[2023-06-04 20:02:25,728] [INFO] GTDB search result was written to GCA_004557935.1_ASM455793v1_genomic.fna/result_gtdb.tsv
[2023-06-04 20:02:25,729] [INFO] ===== GTDB Search completed =====
[2023-06-04 20:02:25,732] [INFO] DFAST_QC result json was written to GCA_004557935.1_ASM455793v1_genomic.fna/dqc_result.json
[2023-06-04 20:02:25,732] [INFO] DFAST_QC completed!
[2023-06-04 20:02:25,732] [INFO] Total running time: 0h0m32s