[2023-06-30 21:54:37,491] [INFO] DFAST_QC pipeline started.
[2023-06-30 21:54:37,496] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 21:54:37,496] [INFO] DQC Reference Directory: /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference
[2023-06-30 21:54:39,087] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 21:54:39,088] [INFO] Task started: Prodigal
[2023-06-30 21:54:39,089] [INFO] Running command: gunzip -c /var/lib/cwl/stgc5c844d3-fb26-4243-8943-220c0ec2f565/GCA_008933075.1_ASM893307v1_genomic.fna.gz | prodigal -d GCA_008933075.1_ASM893307v1_genomic.fna/cds.fna -a GCA_008933075.1_ASM893307v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 21:54:44,982] [INFO] Task succeeded: Prodigal
[2023-06-30 21:54:44,983] [INFO] Task started: HMMsearch
[2023-06-30 21:54:44,983] [INFO] Running command: hmmsearch --tblout GCA_008933075.1_ASM893307v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/reference_markers.hmm GCA_008933075.1_ASM893307v1_genomic.fna/protein.faa > /dev/null
[2023-06-30 21:54:45,223] [INFO] Task succeeded: HMMsearch
[2023-06-30 21:54:45,224] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgc5c844d3-fb26-4243-8943-220c0ec2f565/GCA_008933075.1_ASM893307v1_genomic.fna.gz]
[2023-06-30 21:54:45,248] [INFO] Query marker FASTA was written to GCA_008933075.1_ASM893307v1_genomic.fna/markers.fasta
[2023-06-30 21:54:45,248] [INFO] Task started: Blastn
[2023-06-30 21:54:45,248] [INFO] Running command: blastn -query GCA_008933075.1_ASM893307v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/reference_markers.fasta -out GCA_008933075.1_ASM893307v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 21:54:45,851] [INFO] Task succeeded: Blastn
[2023-06-30 21:54:45,855] [INFO] Selected 15 target genomes.
[2023-06-30 21:54:45,856] [INFO] Target genome list was writen to GCA_008933075.1_ASM893307v1_genomic.fna/target_genomes.txt
[2023-06-30 21:54:45,882] [INFO] Task started: fastANI
[2023-06-30 21:54:45,883] [INFO] Running command: fastANI --query /var/lib/cwl/stgc5c844d3-fb26-4243-8943-220c0ec2f565/GCA_008933075.1_ASM893307v1_genomic.fna.gz --refList GCA_008933075.1_ASM893307v1_genomic.fna/target_genomes.txt --output GCA_008933075.1_ASM893307v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 21:54:57,944] [INFO] Task succeeded: fastANI
[2023-06-30 21:54:57,944] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 21:54:57,945] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 21:54:57,947] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 21:54:57,947] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 21:54:57,947] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 21:54:57,949] [INFO] DFAST Taxonomy check result was written to GCA_008933075.1_ASM893307v1_genomic.fna/tc_result.tsv
[2023-06-30 21:54:57,950] [INFO] ===== Taxonomy check completed =====
[2023-06-30 21:54:57,950] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 21:54:57,950] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/checkm_data
[2023-06-30 21:54:57,953] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 21:54:57,987] [INFO] Task started: CheckM
[2023-06-30 21:54:57,988] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_008933075.1_ASM893307v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_008933075.1_ASM893307v1_genomic.fna/checkm_input GCA_008933075.1_ASM893307v1_genomic.fna/checkm_result
[2023-06-30 21:55:23,292] [INFO] Task succeeded: CheckM
[2023-06-30 21:55:23,294] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 21:55:23,312] [INFO] ===== Completeness check finished =====
[2023-06-30 21:55:23,313] [INFO] ===== Start GTDB Search =====
[2023-06-30 21:55:23,313] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_008933075.1_ASM893307v1_genomic.fna/markers.fasta)
[2023-06-30 21:55:23,313] [INFO] Task started: Blastn
[2023-06-30 21:55:23,313] [INFO] Running command: blastn -query GCA_008933075.1_ASM893307v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg022ca246-b7ae-416a-a42c-a11ad80271f6/dqc_reference/reference_markers_gtdb.fasta -out GCA_008933075.1_ASM893307v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 21:55:24,199] [INFO] Task succeeded: Blastn
[2023-06-30 21:55:24,203] [INFO] Selected 17 target genomes.
[2023-06-30 21:55:24,203] [INFO] Target genome list was writen to GCA_008933075.1_ASM893307v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 21:55:24,216] [INFO] Task started: fastANI
[2023-06-30 21:55:24,217] [INFO] Running command: fastANI --query /var/lib/cwl/stgc5c844d3-fb26-4243-8943-220c0ec2f565/GCA_008933075.1_ASM893307v1_genomic.fna.gz --refList GCA_008933075.1_ASM893307v1_genomic.fna/target_genomes_gtdb.txt --output GCA_008933075.1_ASM893307v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 21:55:34,309] [INFO] Task succeeded: fastANI
[2023-06-30 21:55:34,314] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 21:55:34,314] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903834135.1	s__Palsa-1447 sp903834135	99.5627	500	512	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Opitutales;f__Opitutaceae;g__Palsa-1447	95.0	99.77	99.56	0.97	0.92	44	conclusive
--------------------------------------------------------------------------------
[2023-06-30 21:55:34,317] [INFO] GTDB search result was written to GCA_008933075.1_ASM893307v1_genomic.fna/result_gtdb.tsv
[2023-06-30 21:55:34,317] [INFO] ===== GTDB Search completed =====
[2023-06-30 21:55:34,319] [INFO] DFAST_QC result json was written to GCA_008933075.1_ASM893307v1_genomic.fna/dqc_result.json
[2023-06-30 21:55:34,320] [INFO] DFAST_QC completed!
[2023-06-30 21:55:34,320] [INFO] Total running time: 0h0m57s