[2023-06-17 13:44:23,832] [INFO] DFAST_QC pipeline started.
[2023-06-17 13:44:23,835] [INFO] DFAST_QC version: 0.5.7
[2023-06-17 13:44:23,835] [INFO] DQC Reference Directory: /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference
[2023-06-17 13:44:25,080] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-17 13:44:25,080] [INFO] Task started: Prodigal
[2023-06-17 13:44:25,081] [INFO] Running command: gunzip -c /var/lib/cwl/stg136fb340-3077-442f-b9fc-7bd89e28521b/GCA_009780415.1_ASM978041v1_genomic.fna.gz | prodigal -d GCA_009780415.1_ASM978041v1_genomic.fna/cds.fna -a GCA_009780415.1_ASM978041v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-17 13:44:31,316] [INFO] Task succeeded: Prodigal
[2023-06-17 13:44:31,317] [INFO] Task started: HMMsearch
[2023-06-17 13:44:31,317] [INFO] Running command: hmmsearch --tblout GCA_009780415.1_ASM978041v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/reference_markers.hmm GCA_009780415.1_ASM978041v1_genomic.fna/protein.faa > /dev/null
[2023-06-17 13:44:31,579] [INFO] Task succeeded: HMMsearch
[2023-06-17 13:44:31,581] [INFO] Found 6/6 markers.
[2023-06-17 13:44:31,617] [INFO] Query marker FASTA was written to GCA_009780415.1_ASM978041v1_genomic.fna/markers.fasta
[2023-06-17 13:44:31,618] [INFO] Task started: Blastn
[2023-06-17 13:44:31,618] [INFO] Running command: blastn -query GCA_009780415.1_ASM978041v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/reference_markers.fasta -out GCA_009780415.1_ASM978041v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 13:44:32,304] [INFO] Task succeeded: Blastn
[2023-06-17 13:44:32,309] [INFO] Selected 12 target genomes.
[2023-06-17 13:44:32,309] [INFO] Target genome list was writen to GCA_009780415.1_ASM978041v1_genomic.fna/target_genomes.txt
[2023-06-17 13:44:32,316] [INFO] Task started: fastANI
[2023-06-17 13:44:32,316] [INFO] Running command: fastANI --query /var/lib/cwl/stg136fb340-3077-442f-b9fc-7bd89e28521b/GCA_009780415.1_ASM978041v1_genomic.fna.gz --refList GCA_009780415.1_ASM978041v1_genomic.fna/target_genomes.txt --output GCA_009780415.1_ASM978041v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-17 13:44:39,505] [INFO] Task succeeded: fastANI
[2023-06-17 13:44:39,505] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-17 13:44:39,506] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-17 13:44:39,508] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-17 13:44:39,508] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-17 13:44:39,509] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-17 13:44:39,511] [INFO] DFAST Taxonomy check result was written to GCA_009780415.1_ASM978041v1_genomic.fna/tc_result.tsv
[2023-06-17 13:44:39,512] [INFO] ===== Taxonomy check completed =====
[2023-06-17 13:44:39,513] [INFO] ===== Start completeness check using CheckM =====
[2023-06-17 13:44:39,513] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/checkm_data
[2023-06-17 13:44:39,518] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-17 13:44:39,548] [INFO] Task started: CheckM
[2023-06-17 13:44:39,549] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_009780415.1_ASM978041v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_009780415.1_ASM978041v1_genomic.fna/checkm_input GCA_009780415.1_ASM978041v1_genomic.fna/checkm_result
[2023-06-17 13:45:04,170] [INFO] Task succeeded: CheckM
[2023-06-17 13:45:04,171] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-17 13:45:04,194] [INFO] ===== Completeness check finished =====
[2023-06-17 13:45:04,194] [INFO] ===== Start GTDB Search =====
[2023-06-17 13:45:04,194] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_009780415.1_ASM978041v1_genomic.fna/markers.fasta)
[2023-06-17 13:45:04,195] [INFO] Task started: Blastn
[2023-06-17 13:45:04,195] [INFO] Running command: blastn -query GCA_009780415.1_ASM978041v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg797e42a0-85fc-4d18-a714-3ab0f2e7ee5e/dqc_reference/reference_markers_gtdb.fasta -out GCA_009780415.1_ASM978041v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 13:45:05,240] [INFO] Task succeeded: Blastn
[2023-06-17 13:45:05,244] [INFO] Selected 26 target genomes.
[2023-06-17 13:45:05,245] [INFO] Target genome list was writen to GCA_009780415.1_ASM978041v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-17 13:45:05,275] [INFO] Task started: fastANI
[2023-06-17 13:45:05,276] [INFO] Running command: fastANI --query /var/lib/cwl/stg136fb340-3077-442f-b9fc-7bd89e28521b/GCA_009780415.1_ASM978041v1_genomic.fna.gz --refList GCA_009780415.1_ASM978041v1_genomic.fna/target_genomes_gtdb.txt --output GCA_009780415.1_ASM978041v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-17 13:45:17,133] [INFO] Task succeeded: fastANI
[2023-06-17 13:45:17,138] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-17 13:45:17,139] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009780415.1	s__WRAC01 sp009780415	100.0	793	800	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__WRAX01;g__WRAC01	95.0	99.99	99.99	0.98	0.98	2	conclusive
--------------------------------------------------------------------------------
[2023-06-17 13:45:17,141] [INFO] GTDB search result was written to GCA_009780415.1_ASM978041v1_genomic.fna/result_gtdb.tsv
[2023-06-17 13:45:17,142] [INFO] ===== GTDB Search completed =====
[2023-06-17 13:45:17,145] [INFO] DFAST_QC result json was written to GCA_009780415.1_ASM978041v1_genomic.fna/dqc_result.json
[2023-06-17 13:45:17,145] [INFO] DFAST_QC completed!
[2023-06-17 13:45:17,145] [INFO] Total running time: 0h0m53s