[2023-06-05 07:09:45,239] [INFO] DFAST_QC pipeline started.
[2023-06-05 07:09:45,243] [INFO] DFAST_QC version: 0.5.7
[2023-06-05 07:09:45,243] [INFO] DQC Reference Directory: /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference
[2023-06-05 07:09:46,844] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-05 07:09:46,845] [INFO] Task started: Prodigal
[2023-06-05 07:09:46,845] [INFO] Running command: gunzip -c /var/lib/cwl/stg0fae5ca9-c2c5-4dc4-981c-eb26b93917ee/GCA_010021325.1_ASM1002132v1_genomic.fna.gz | prodigal -d GCA_010021325.1_ASM1002132v1_genomic.fna/cds.fna -a GCA_010021325.1_ASM1002132v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-05 07:09:50,535] [INFO] Task succeeded: Prodigal
[2023-06-05 07:09:50,535] [INFO] Task started: HMMsearch
[2023-06-05 07:09:50,535] [INFO] Running command: hmmsearch --tblout GCA_010021325.1_ASM1002132v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/reference_markers.hmm GCA_010021325.1_ASM1002132v1_genomic.fna/protein.faa > /dev/null
[2023-06-05 07:09:50,756] [INFO] Task succeeded: HMMsearch
[2023-06-05 07:09:50,758] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg0fae5ca9-c2c5-4dc4-981c-eb26b93917ee/GCA_010021325.1_ASM1002132v1_genomic.fna.gz]
[2023-06-05 07:09:50,777] [INFO] Query marker FASTA was written to GCA_010021325.1_ASM1002132v1_genomic.fna/markers.fasta
[2023-06-05 07:09:50,778] [INFO] Task started: Blastn
[2023-06-05 07:09:50,778] [INFO] Running command: blastn -query GCA_010021325.1_ASM1002132v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/reference_markers.fasta -out GCA_010021325.1_ASM1002132v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 07:09:51,369] [INFO] Task succeeded: Blastn
[2023-06-05 07:09:51,373] [INFO] Selected 15 target genomes.
[2023-06-05 07:09:51,373] [INFO] Target genome list was writen to GCA_010021325.1_ASM1002132v1_genomic.fna/target_genomes.txt
[2023-06-05 07:09:51,374] [INFO] Task started: fastANI
[2023-06-05 07:09:51,374] [INFO] Running command: fastANI --query /var/lib/cwl/stg0fae5ca9-c2c5-4dc4-981c-eb26b93917ee/GCA_010021325.1_ASM1002132v1_genomic.fna.gz --refList GCA_010021325.1_ASM1002132v1_genomic.fna/target_genomes.txt --output GCA_010021325.1_ASM1002132v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-05 07:09:59,137] [INFO] Task succeeded: fastANI
[2023-06-05 07:09:59,137] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-05 07:09:59,138] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-05 07:09:59,140] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-05 07:09:59,140] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-05 07:09:59,140] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-05 07:09:59,142] [INFO] DFAST Taxonomy check result was written to GCA_010021325.1_ASM1002132v1_genomic.fna/tc_result.tsv
[2023-06-05 07:09:59,143] [INFO] ===== Taxonomy check completed =====
[2023-06-05 07:09:59,143] [INFO] ===== Start completeness check using CheckM =====
[2023-06-05 07:09:59,143] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/checkm_data
[2023-06-05 07:09:59,145] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-05 07:09:59,168] [INFO] Task started: CheckM
[2023-06-05 07:09:59,168] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_010021325.1_ASM1002132v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_010021325.1_ASM1002132v1_genomic.fna/checkm_input GCA_010021325.1_ASM1002132v1_genomic.fna/checkm_result
[2023-06-05 07:10:18,824] [INFO] Task succeeded: CheckM
[2023-06-05 07:10:18,826] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 1.39%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-05 07:10:18,848] [INFO] ===== Completeness check finished =====
[2023-06-05 07:10:18,848] [INFO] ===== Start GTDB Search =====
[2023-06-05 07:10:18,849] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_010021325.1_ASM1002132v1_genomic.fna/markers.fasta)
[2023-06-05 07:10:18,849] [INFO] Task started: Blastn
[2023-06-05 07:10:18,850] [INFO] Running command: blastn -query GCA_010021325.1_ASM1002132v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg394d1c40-d009-4c12-86db-7dad64caa958/dqc_reference/reference_markers_gtdb.fasta -out GCA_010021325.1_ASM1002132v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 07:10:19,597] [INFO] Task succeeded: Blastn
[2023-06-05 07:10:19,601] [INFO] Selected 11 target genomes.
[2023-06-05 07:10:19,601] [INFO] Target genome list was writen to GCA_010021325.1_ASM1002132v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-05 07:10:19,602] [INFO] Task started: fastANI
[2023-06-05 07:10:19,602] [INFO] Running command: fastANI --query /var/lib/cwl/stg0fae5ca9-c2c5-4dc4-981c-eb26b93917ee/GCA_010021325.1_ASM1002132v1_genomic.fna.gz --refList GCA_010021325.1_ASM1002132v1_genomic.fna/target_genomes_gtdb.txt --output GCA_010021325.1_ASM1002132v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-05 07:10:22,475] [INFO] Task succeeded: fastANI
[2023-06-05 07:10:22,488] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-05 07:10:22,488] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003535455.1	s__BACL27 sp003535455	98.6444	215	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	98.51	98.35	0.83	0.76	7	conclusive
GCA_009923685.1	s__BACL27 sp009923685	87.5983	106	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002737635.1	s__BACL27 sp002737635	81.4313	121	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014190055.1	s__BACL27 sp014190055	80.5221	140	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	95.53	95.36	0.86	0.77	8	-
GCA_005805045.1	s__BACL27 sp005805045	79.7368	106	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	99.25	99.23	0.81	0.81	3	-
GCA_009927565.1	s__BACL27 sp009927565	79.2219	100	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016462185.1	s__BACL27 sp016462185	78.63	114	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	98.92	98.92	0.88	0.88	2	-
GCA_016870935.1	s__BACL27 sp016870935	78.2752	93	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014190295.1	s__BACL27 sp014190295	77.9723	87	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016871295.1	s__BACL27 sp016871295	77.5297	91	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016463105.1	s__BACL27 sp016463105	77.173	65	253	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-05 07:10:22,492] [INFO] GTDB search result was written to GCA_010021325.1_ASM1002132v1_genomic.fna/result_gtdb.tsv
[2023-06-05 07:10:22,493] [INFO] ===== GTDB Search completed =====
[2023-06-05 07:10:22,496] [INFO] DFAST_QC result json was written to GCA_010021325.1_ASM1002132v1_genomic.fna/dqc_result.json
[2023-06-05 07:10:22,496] [INFO] DFAST_QC completed!
[2023-06-05 07:10:22,496] [INFO] Total running time: 0h0m37s