[2023-06-29 19:35:36,213] [INFO] DFAST_QC pipeline started.
[2023-06-29 19:35:36,215] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 19:35:36,215] [INFO] DQC Reference Directory: /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference
[2023-06-29 19:35:37,483] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 19:35:37,484] [INFO] Task started: Prodigal
[2023-06-29 19:35:37,484] [INFO] Running command: gunzip -c /var/lib/cwl/stgf58d97bf-0547-417f-ab7f-dc024ce08e40/GCA_011042535.1_ASM1104253v1_genomic.fna.gz | prodigal -d GCA_011042535.1_ASM1104253v1_genomic.fna/cds.fna -a GCA_011042535.1_ASM1104253v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 19:35:41,516] [INFO] Task succeeded: Prodigal
[2023-06-29 19:35:41,517] [INFO] Task started: HMMsearch
[2023-06-29 19:35:41,517] [INFO] Running command: hmmsearch --tblout GCA_011042535.1_ASM1104253v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/reference_markers.hmm GCA_011042535.1_ASM1104253v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 19:35:41,729] [INFO] Task succeeded: HMMsearch
[2023-06-29 19:35:41,731] [WARNING] Found 2/6 markers. [/var/lib/cwl/stgf58d97bf-0547-417f-ab7f-dc024ce08e40/GCA_011042535.1_ASM1104253v1_genomic.fna.gz]
[2023-06-29 19:35:41,752] [INFO] Query marker FASTA was written to GCA_011042535.1_ASM1104253v1_genomic.fna/markers.fasta
[2023-06-29 19:35:41,752] [INFO] Task started: Blastn
[2023-06-29 19:35:41,753] [INFO] Running command: blastn -query GCA_011042535.1_ASM1104253v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/reference_markers.fasta -out GCA_011042535.1_ASM1104253v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 19:35:42,290] [INFO] Task succeeded: Blastn
[2023-06-29 19:35:42,294] [INFO] Selected 5 target genomes.
[2023-06-29 19:35:42,294] [INFO] Target genome list was writen to GCA_011042535.1_ASM1104253v1_genomic.fna/target_genomes.txt
[2023-06-29 19:35:42,296] [INFO] Task started: fastANI
[2023-06-29 19:35:42,296] [INFO] Running command: fastANI --query /var/lib/cwl/stgf58d97bf-0547-417f-ab7f-dc024ce08e40/GCA_011042535.1_ASM1104253v1_genomic.fna.gz --refList GCA_011042535.1_ASM1104253v1_genomic.fna/target_genomes.txt --output GCA_011042535.1_ASM1104253v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 19:35:43,600] [INFO] Task succeeded: fastANI
[2023-06-29 19:35:43,601] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 19:35:43,601] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 19:35:43,602] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 19:35:43,602] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 19:35:43,603] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 19:35:43,604] [INFO] DFAST Taxonomy check result was written to GCA_011042535.1_ASM1104253v1_genomic.fna/tc_result.tsv
[2023-06-29 19:35:43,605] [INFO] ===== Taxonomy check completed =====
[2023-06-29 19:35:43,605] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 19:35:43,605] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/checkm_data
[2023-06-29 19:35:43,608] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 19:35:43,622] [INFO] Task started: CheckM
[2023-06-29 19:35:43,622] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_011042535.1_ASM1104253v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_011042535.1_ASM1104253v1_genomic.fna/checkm_input GCA_011042535.1_ASM1104253v1_genomic.fna/checkm_result
[2023-06-29 19:36:02,618] [INFO] Task succeeded: CheckM
[2023-06-29 19:36:02,619] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 19:36:02,639] [INFO] ===== Completeness check finished =====
[2023-06-29 19:36:02,639] [INFO] ===== Start GTDB Search =====
[2023-06-29 19:36:02,640] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_011042535.1_ASM1104253v1_genomic.fna/markers.fasta)
[2023-06-29 19:36:02,640] [INFO] Task started: Blastn
[2023-06-29 19:36:02,640] [INFO] Running command: blastn -query GCA_011042535.1_ASM1104253v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2fd2cc26-0251-4eb3-9850-1f71a77d6d07/dqc_reference/reference_markers_gtdb.fasta -out GCA_011042535.1_ASM1104253v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 19:36:03,223] [INFO] Task succeeded: Blastn
[2023-06-29 19:36:03,235] [INFO] Selected 6 target genomes.
[2023-06-29 19:36:03,235] [INFO] Target genome list was writen to GCA_011042535.1_ASM1104253v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 19:36:03,243] [INFO] Task started: fastANI
[2023-06-29 19:36:03,244] [INFO] Running command: fastANI --query /var/lib/cwl/stgf58d97bf-0547-417f-ab7f-dc024ce08e40/GCA_011042535.1_ASM1104253v1_genomic.fna.gz --refList GCA_011042535.1_ASM1104253v1_genomic.fna/target_genomes_gtdb.txt --output GCA_011042535.1_ASM1104253v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 19:36:05,258] [INFO] Task succeeded: fastANI
[2023-06-29 19:36:05,261] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 19:36:05,261] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003649695.1	s__B87-G9 sp003649695	98.921	148	214	d__Archaea;p__Thermoplasmatota;c__Thermoplasmata;o__B87-G9;f__B87-G9;g__B87-G9	95.0	98.92	98.92	0.69	0.69	2	conclusive
--------------------------------------------------------------------------------
[2023-06-29 19:36:05,264] [INFO] GTDB search result was written to GCA_011042535.1_ASM1104253v1_genomic.fna/result_gtdb.tsv
[2023-06-29 19:36:05,265] [INFO] ===== GTDB Search completed =====
[2023-06-29 19:36:05,267] [INFO] DFAST_QC result json was written to GCA_011042535.1_ASM1104253v1_genomic.fna/dqc_result.json
[2023-06-29 19:36:05,267] [INFO] DFAST_QC completed!
[2023-06-29 19:36:05,267] [INFO] Total running time: 0h0m29s