[2023-06-29 15:04:16,497] [INFO] DFAST_QC pipeline started.
[2023-06-29 15:04:16,499] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 15:04:16,499] [INFO] DQC Reference Directory: /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference
[2023-06-29 15:04:17,765] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 15:04:17,766] [INFO] Task started: Prodigal
[2023-06-29 15:04:17,766] [INFO] Running command: gunzip -c /var/lib/cwl/stg551c2659-57a0-47e5-96e3-3258c55d1847/GCA_011042615.1_ASM1104261v1_genomic.fna.gz | prodigal -d GCA_011042615.1_ASM1104261v1_genomic.fna/cds.fna -a GCA_011042615.1_ASM1104261v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 15:04:20,506] [INFO] Task succeeded: Prodigal
[2023-06-29 15:04:20,507] [INFO] Task started: HMMsearch
[2023-06-29 15:04:20,507] [INFO] Running command: hmmsearch --tblout GCA_011042615.1_ASM1104261v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/reference_markers.hmm GCA_011042615.1_ASM1104261v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 15:04:20,695] [INFO] Task succeeded: HMMsearch
[2023-06-29 15:04:20,697] [WARNING] Found 1/6 markers. [/var/lib/cwl/stg551c2659-57a0-47e5-96e3-3258c55d1847/GCA_011042615.1_ASM1104261v1_genomic.fna.gz]
[2023-06-29 15:04:20,716] [INFO] Query marker FASTA was written to GCA_011042615.1_ASM1104261v1_genomic.fna/markers.fasta
[2023-06-29 15:04:20,717] [INFO] Task started: Blastn
[2023-06-29 15:04:20,717] [INFO] Running command: blastn -query GCA_011042615.1_ASM1104261v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/reference_markers.fasta -out GCA_011042615.1_ASM1104261v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 15:04:21,180] [INFO] Task succeeded: Blastn
[2023-06-29 15:04:21,183] [INFO] Selected 1 target genomes.
[2023-06-29 15:04:21,184] [INFO] Target genome list was writen to GCA_011042615.1_ASM1104261v1_genomic.fna/target_genomes.txt
[2023-06-29 15:04:21,185] [INFO] Task started: fastANI
[2023-06-29 15:04:21,185] [INFO] Running command: fastANI --query /var/lib/cwl/stg551c2659-57a0-47e5-96e3-3258c55d1847/GCA_011042615.1_ASM1104261v1_genomic.fna.gz --refList GCA_011042615.1_ASM1104261v1_genomic.fna/target_genomes.txt --output GCA_011042615.1_ASM1104261v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 15:04:21,589] [INFO] Task succeeded: fastANI
[2023-06-29 15:04:21,589] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 15:04:21,590] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 15:04:21,592] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 15:04:21,592] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 15:04:21,592] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 15:04:21,594] [INFO] DFAST Taxonomy check result was written to GCA_011042615.1_ASM1104261v1_genomic.fna/tc_result.tsv
[2023-06-29 15:04:21,595] [INFO] ===== Taxonomy check completed =====
[2023-06-29 15:04:21,595] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 15:04:21,595] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/checkm_data
[2023-06-29 15:04:21,598] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 15:04:21,615] [INFO] Task started: CheckM
[2023-06-29 15:04:21,616] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_011042615.1_ASM1104261v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_011042615.1_ASM1104261v1_genomic.fna/checkm_input GCA_011042615.1_ASM1104261v1_genomic.fna/checkm_result
[2023-06-29 15:04:37,831] [INFO] Task succeeded: CheckM
[2023-06-29 15:04:37,833] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 71.19%
Contamintation: 2.08%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-06-29 15:04:37,885] [INFO] ===== Completeness check finished =====
[2023-06-29 15:04:37,885] [INFO] ===== Start GTDB Search =====
[2023-06-29 15:04:37,886] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_011042615.1_ASM1104261v1_genomic.fna/markers.fasta)
[2023-06-29 15:04:37,886] [INFO] Task started: Blastn
[2023-06-29 15:04:37,886] [INFO] Running command: blastn -query GCA_011042615.1_ASM1104261v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4727b965-e6f0-41da-963b-00d0605ea6e9/dqc_reference/reference_markers_gtdb.fasta -out GCA_011042615.1_ASM1104261v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 15:04:38,323] [INFO] Task succeeded: Blastn
[2023-06-29 15:04:38,327] [INFO] Selected 2 target genomes.
[2023-06-29 15:04:38,327] [INFO] Target genome list was writen to GCA_011042615.1_ASM1104261v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 15:04:38,330] [INFO] Task started: fastANI
[2023-06-29 15:04:38,330] [INFO] Running command: fastANI --query /var/lib/cwl/stg551c2659-57a0-47e5-96e3-3258c55d1847/GCA_011042615.1_ASM1104261v1_genomic.fna.gz --refList GCA_011042615.1_ASM1104261v1_genomic.fna/target_genomes_gtdb.txt --output GCA_011042615.1_ASM1104261v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 15:04:39,027] [INFO] Task succeeded: fastANI
[2023-06-29 15:04:39,031] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 15:04:39,031] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_013572355.1	s__Methanogaster sp013572355	80.4433	132	281	d__Archaea;p__Halobacteriota;c__Methanosarcinia;o__Methanosarcinales;f__Methanogasteraceae;g__Methanogaster	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-29 15:04:39,034] [INFO] GTDB search result was written to GCA_011042615.1_ASM1104261v1_genomic.fna/result_gtdb.tsv
[2023-06-29 15:04:39,034] [INFO] ===== GTDB Search completed =====
[2023-06-29 15:04:39,037] [INFO] DFAST_QC result json was written to GCA_011042615.1_ASM1104261v1_genomic.fna/dqc_result.json
[2023-06-29 15:04:39,037] [INFO] DFAST_QC completed!
[2023-06-29 15:04:39,038] [INFO] Total running time: 0h0m23s