[2023-06-29 00:23:05,331] [INFO] DFAST_QC pipeline started.
[2023-06-29 00:23:05,334] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 00:23:05,334] [INFO] DQC Reference Directory: /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference
[2023-06-29 00:23:06,586] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 00:23:06,587] [INFO] Task started: Prodigal
[2023-06-29 00:23:06,588] [INFO] Running command: gunzip -c /var/lib/cwl/stgfdee40c3-1278-4554-beff-985c82836c71/GCA_012032135.1_ASM1203213v1_genomic.fna.gz | prodigal -d GCA_012032135.1_ASM1203213v1_genomic.fna/cds.fna -a GCA_012032135.1_ASM1203213v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 00:23:16,437] [INFO] Task succeeded: Prodigal
[2023-06-29 00:23:16,437] [INFO] Task started: HMMsearch
[2023-06-29 00:23:16,438] [INFO] Running command: hmmsearch --tblout GCA_012032135.1_ASM1203213v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/reference_markers.hmm GCA_012032135.1_ASM1203213v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 00:23:16,733] [INFO] Task succeeded: HMMsearch
[2023-06-29 00:23:16,734] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgfdee40c3-1278-4554-beff-985c82836c71/GCA_012032135.1_ASM1203213v1_genomic.fna.gz]
[2023-06-29 00:23:16,773] [INFO] Query marker FASTA was written to GCA_012032135.1_ASM1203213v1_genomic.fna/markers.fasta
[2023-06-29 00:23:16,774] [INFO] Task started: Blastn
[2023-06-29 00:23:16,774] [INFO] Running command: blastn -query GCA_012032135.1_ASM1203213v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/reference_markers.fasta -out GCA_012032135.1_ASM1203213v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 00:23:17,386] [INFO] Task succeeded: Blastn
[2023-06-29 00:23:17,391] [INFO] Selected 16 target genomes.
[2023-06-29 00:23:17,392] [INFO] Target genome list was writen to GCA_012032135.1_ASM1203213v1_genomic.fna/target_genomes.txt
[2023-06-29 00:23:17,412] [INFO] Task started: fastANI
[2023-06-29 00:23:17,412] [INFO] Running command: fastANI --query /var/lib/cwl/stgfdee40c3-1278-4554-beff-985c82836c71/GCA_012032135.1_ASM1203213v1_genomic.fna.gz --refList GCA_012032135.1_ASM1203213v1_genomic.fna/target_genomes.txt --output GCA_012032135.1_ASM1203213v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 00:23:30,834] [INFO] Task succeeded: fastANI
[2023-06-29 00:23:30,834] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 00:23:30,834] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 00:23:30,836] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 00:23:30,836] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 00:23:30,836] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 00:23:30,838] [INFO] DFAST Taxonomy check result was written to GCA_012032135.1_ASM1203213v1_genomic.fna/tc_result.tsv
[2023-06-29 00:23:30,841] [INFO] ===== Taxonomy check completed =====
[2023-06-29 00:23:30,841] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 00:23:30,841] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/checkm_data
[2023-06-29 00:23:30,844] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 00:23:30,894] [INFO] Task started: CheckM
[2023-06-29 00:23:30,894] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_012032135.1_ASM1203213v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_012032135.1_ASM1203213v1_genomic.fna/checkm_input GCA_012032135.1_ASM1203213v1_genomic.fna/checkm_result
[2023-06-29 00:24:04,838] [INFO] Task succeeded: CheckM
[2023-06-29 00:24:04,841] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 88.31%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 00:24:04,868] [INFO] ===== Completeness check finished =====
[2023-06-29 00:24:04,868] [INFO] ===== Start GTDB Search =====
[2023-06-29 00:24:04,869] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_012032135.1_ASM1203213v1_genomic.fna/markers.fasta)
[2023-06-29 00:24:04,869] [INFO] Task started: Blastn
[2023-06-29 00:24:04,869] [INFO] Running command: blastn -query GCA_012032135.1_ASM1203213v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg16c912f6-8b74-4ed1-a816-28bc561f0245/dqc_reference/reference_markers_gtdb.fasta -out GCA_012032135.1_ASM1203213v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 00:24:05,649] [INFO] Task succeeded: Blastn
[2023-06-29 00:24:05,653] [INFO] Selected 25 target genomes.
[2023-06-29 00:24:05,654] [INFO] Target genome list was writen to GCA_012032135.1_ASM1203213v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 00:24:05,698] [INFO] Task started: fastANI
[2023-06-29 00:24:05,698] [INFO] Running command: fastANI --query /var/lib/cwl/stgfdee40c3-1278-4554-beff-985c82836c71/GCA_012032135.1_ASM1203213v1_genomic.fna.gz --refList GCA_012032135.1_ASM1203213v1_genomic.fna/target_genomes_gtdb.txt --output GCA_012032135.1_ASM1203213v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 00:24:24,285] [INFO] Task succeeded: fastANI
[2023-06-29 00:24:24,290] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 00:24:24,291] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_012032135.1	s__JAAURS01 sp012032135	99.9999	1264	1291	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Thermosynechococcales;f__Thermosynechococcaceae;g__JAAURS01	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-29 00:24:24,293] [INFO] GTDB search result was written to GCA_012032135.1_ASM1203213v1_genomic.fna/result_gtdb.tsv
[2023-06-29 00:24:24,295] [INFO] ===== GTDB Search completed =====
[2023-06-29 00:24:24,297] [INFO] DFAST_QC result json was written to GCA_012032135.1_ASM1203213v1_genomic.fna/dqc_result.json
[2023-06-29 00:24:24,298] [INFO] DFAST_QC completed!
[2023-06-29 00:24:24,298] [INFO] Total running time: 0h1m19s