[2023-06-19 12:10:14,945] [INFO] DFAST_QC pipeline started.
[2023-06-19 12:10:14,947] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 12:10:14,947] [INFO] DQC Reference Directory: /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference
[2023-06-19 12:10:18,172] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 12:10:18,203] [INFO] Task started: Prodigal
[2023-06-19 12:10:18,204] [INFO] Running command: gunzip -c /var/lib/cwl/stg100478d1-79c5-44bb-a392-8cacf666a354/GCA_013289115.1_ASM1328911v1_genomic.fna.gz | prodigal -d GCA_013289115.1_ASM1328911v1_genomic.fna/cds.fna -a GCA_013289115.1_ASM1328911v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 12:10:20,508] [INFO] Task succeeded: Prodigal
[2023-06-19 12:10:20,509] [INFO] Task started: HMMsearch
[2023-06-19 12:10:20,509] [INFO] Running command: hmmsearch --tblout GCA_013289115.1_ASM1328911v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/reference_markers.hmm GCA_013289115.1_ASM1328911v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 12:10:20,752] [INFO] Task succeeded: HMMsearch
[2023-06-19 12:10:20,753] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg100478d1-79c5-44bb-a392-8cacf666a354/GCA_013289115.1_ASM1328911v1_genomic.fna.gz]
[2023-06-19 12:10:20,772] [INFO] Query marker FASTA was written to GCA_013289115.1_ASM1328911v1_genomic.fna/markers.fasta
[2023-06-19 12:10:20,773] [INFO] Task started: Blastn
[2023-06-19 12:10:20,773] [INFO] Running command: blastn -query GCA_013289115.1_ASM1328911v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/reference_markers.fasta -out GCA_013289115.1_ASM1328911v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 12:10:22,623] [INFO] Task succeeded: Blastn
[2023-06-19 12:10:22,628] [INFO] Selected 13 target genomes.
[2023-06-19 12:10:22,628] [INFO] Target genome list was writen to GCA_013289115.1_ASM1328911v1_genomic.fna/target_genomes.txt
[2023-06-19 12:10:22,633] [INFO] Task started: fastANI
[2023-06-19 12:10:22,633] [INFO] Running command: fastANI --query /var/lib/cwl/stg100478d1-79c5-44bb-a392-8cacf666a354/GCA_013289115.1_ASM1328911v1_genomic.fna.gz --refList GCA_013289115.1_ASM1328911v1_genomic.fna/target_genomes.txt --output GCA_013289115.1_ASM1328911v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 12:10:29,084] [INFO] Task succeeded: fastANI
[2023-06-19 12:10:29,084] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 12:10:29,084] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 12:10:29,086] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 12:10:29,086] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 12:10:29,086] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 12:10:29,088] [INFO] DFAST Taxonomy check result was written to GCA_013289115.1_ASM1328911v1_genomic.fna/tc_result.tsv
[2023-06-19 12:10:29,088] [INFO] ===== Taxonomy check completed =====
[2023-06-19 12:10:29,089] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 12:10:29,089] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/checkm_data
[2023-06-19 12:10:29,092] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 12:10:29,110] [INFO] Task started: CheckM
[2023-06-19 12:10:29,111] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_013289115.1_ASM1328911v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_013289115.1_ASM1328911v1_genomic.fna/checkm_input GCA_013289115.1_ASM1328911v1_genomic.fna/checkm_result
[2023-06-19 12:10:44,598] [INFO] Task succeeded: CheckM
[2023-06-19 12:10:44,600] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 12:10:44,620] [INFO] ===== Completeness check finished =====
[2023-06-19 12:10:44,621] [INFO] ===== Start GTDB Search =====
[2023-06-19 12:10:44,621] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_013289115.1_ASM1328911v1_genomic.fna/markers.fasta)
[2023-06-19 12:10:44,622] [INFO] Task started: Blastn
[2023-06-19 12:10:44,622] [INFO] Running command: blastn -query GCA_013289115.1_ASM1328911v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1e73a745-76f4-4d66-9d31-d93213e71dd1/dqc_reference/reference_markers_gtdb.fasta -out GCA_013289115.1_ASM1328911v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 12:10:45,386] [INFO] Task succeeded: Blastn
[2023-06-19 12:10:45,393] [INFO] Selected 17 target genomes.
[2023-06-19 12:10:45,393] [INFO] Target genome list was writen to GCA_013289115.1_ASM1328911v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 12:10:45,404] [INFO] Task started: fastANI
[2023-06-19 12:10:45,405] [INFO] Running command: fastANI --query /var/lib/cwl/stg100478d1-79c5-44bb-a392-8cacf666a354/GCA_013289115.1_ASM1328911v1_genomic.fna.gz --refList GCA_013289115.1_ASM1328911v1_genomic.fna/target_genomes_gtdb.txt --output GCA_013289115.1_ASM1328911v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 12:10:52,609] [INFO] Task succeeded: fastANI
[2023-06-19 12:10:52,614] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 12:10:52,614] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_013289115.1	s__JABDAQ01 sp013289115	100.0	357	361	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Diplorickettsiales;f__Diplorickettsiaceae;g__JABDAQ01	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-19 12:10:52,617] [INFO] GTDB search result was written to GCA_013289115.1_ASM1328911v1_genomic.fna/result_gtdb.tsv
[2023-06-19 12:10:52,618] [INFO] ===== GTDB Search completed =====
[2023-06-19 12:10:52,620] [INFO] DFAST_QC result json was written to GCA_013289115.1_ASM1328911v1_genomic.fna/dqc_result.json
[2023-06-19 12:10:52,620] [INFO] DFAST_QC completed!
[2023-06-19 12:10:52,620] [INFO] Total running time: 0h0m38s