[2023-06-29 06:10:51,850] [INFO] DFAST_QC pipeline started.
[2023-06-29 06:10:51,853] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 06:10:51,853] [INFO] DQC Reference Directory: /var/lib/cwl/stg68089f08-f8ca-4501-b7c6-5c2a28498591/dqc_reference
[2023-06-29 06:10:54,285] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 06:10:54,286] [INFO] Task started: Prodigal
[2023-06-29 06:10:54,286] [INFO] Running command: gunzip -c /var/lib/cwl/stgd79e9e63-c294-49ff-9bc5-3e08f187f8f5/GCA_015521175.1_ASM1552117v1_genomic.fna.gz | prodigal -d GCA_015521175.1_ASM1552117v1_genomic.fna/cds.fna -a GCA_015521175.1_ASM1552117v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 06:10:56,632] [INFO] Task succeeded: Prodigal
[2023-06-29 06:10:56,633] [INFO] Task started: HMMsearch
[2023-06-29 06:10:56,633] [INFO] Running command: hmmsearch --tblout GCA_015521175.1_ASM1552117v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg68089f08-f8ca-4501-b7c6-5c2a28498591/dqc_reference/reference_markers.hmm GCA_015521175.1_ASM1552117v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 06:10:56,823] [INFO] Task succeeded: HMMsearch
[2023-06-29 06:10:56,825] [WARNING] Found 1/6 markers. [/var/lib/cwl/stgd79e9e63-c294-49ff-9bc5-3e08f187f8f5/GCA_015521175.1_ASM1552117v1_genomic.fna.gz]
[2023-06-29 06:10:56,845] [INFO] Query marker FASTA was written to GCA_015521175.1_ASM1552117v1_genomic.fna/markers.fasta
[2023-06-29 06:10:56,845] [INFO] Task started: Blastn
[2023-06-29 06:10:56,845] [INFO] Running command: blastn -query GCA_015521175.1_ASM1552117v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg68089f08-f8ca-4501-b7c6-5c2a28498591/dqc_reference/reference_markers.fasta -out GCA_015521175.1_ASM1552117v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 06:10:59,201] [INFO] Task succeeded: Blastn
[2023-06-29 06:10:59,204] [INFO] Selected 0 target genomes.
[2023-06-29 06:10:59,204] [INFO] Target genome list was writen to GCA_015521175.1_ASM1552117v1_genomic.fna/target_genomes.txt
[2023-06-29 06:10:59,204] [ERROR] File is empty. [GCA_015521175.1_ASM1552117v1_genomic.fna/target_genomes.txt]
[2023-06-29 06:10:59,204] [ERROR] Task failed. No target genome found.
[2023-06-29 06:10:59,205] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 06:10:59,205] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg68089f08-f8ca-4501-b7c6-5c2a28498591/dqc_reference/checkm_data
[2023-06-29 06:10:59,208] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 06:10:59,228] [INFO] Task started: CheckM
[2023-06-29 06:10:59,228] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_015521175.1_ASM1552117v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_015521175.1_ASM1552117v1_genomic.fna/checkm_input GCA_015521175.1_ASM1552117v1_genomic.fna/checkm_result
[2023-06-29 06:11:16,591] [INFO] Task succeeded: CheckM
[2023-06-29 06:11:16,592] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 3.47%
Strain heterogeneity: 75.00%
--------------------------------------------------------------------------------
[2023-06-29 06:11:16,613] [INFO] ===== Completeness check finished =====
[2023-06-29 06:11:16,613] [INFO] ===== Start GTDB Search =====
[2023-06-29 06:11:16,613] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_015521175.1_ASM1552117v1_genomic.fna/markers.fasta)
[2023-06-29 06:11:16,613] [INFO] Task started: Blastn
[2023-06-29 06:11:16,614] [INFO] Running command: blastn -query GCA_015521175.1_ASM1552117v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg68089f08-f8ca-4501-b7c6-5c2a28498591/dqc_reference/reference_markers_gtdb.fasta -out GCA_015521175.1_ASM1552117v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 06:11:17,092] [INFO] Task succeeded: Blastn
[2023-06-29 06:11:17,096] [INFO] Selected 4 target genomes.
[2023-06-29 06:11:17,097] [INFO] Target genome list was writen to GCA_015521175.1_ASM1552117v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 06:11:17,103] [INFO] Task started: fastANI
[2023-06-29 06:11:17,103] [INFO] Running command: fastANI --query /var/lib/cwl/stgd79e9e63-c294-49ff-9bc5-3e08f187f8f5/GCA_015521175.1_ASM1552117v1_genomic.fna.gz --refList GCA_015521175.1_ASM1552117v1_genomic.fna/target_genomes_gtdb.txt --output GCA_015521175.1_ASM1552117v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 06:11:18,090] [INFO] Task succeeded: fastANI
[2023-06-29 06:11:18,095] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 06:11:18,096] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015521175.1	s__JAADGB01 sp015521175	100.0	348	354	d__Archaea;p__Thermoproteota;c__Thermoproteia;o__Sulfolobales;f__Acidilobaceae;g__JAADGB01	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-29 06:11:18,098] [INFO] GTDB search result was written to GCA_015521175.1_ASM1552117v1_genomic.fna/result_gtdb.tsv
[2023-06-29 06:11:18,099] [INFO] ===== GTDB Search completed =====
[2023-06-29 06:11:18,102] [INFO] DFAST_QC result json was written to GCA_015521175.1_ASM1552117v1_genomic.fna/dqc_result.json
[2023-06-29 06:11:18,102] [INFO] DFAST_QC completed!
[2023-06-29 06:11:18,102] [INFO] Total running time: 0h0m26s