[2023-06-30 12:52:09,051] [INFO] DFAST_QC pipeline started.
[2023-06-30 12:52:09,057] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 12:52:09,057] [INFO] DQC Reference Directory: /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference
[2023-06-30 12:52:11,330] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 12:52:11,331] [INFO] Task started: Prodigal
[2023-06-30 12:52:11,331] [INFO] Running command: gunzip -c /var/lib/cwl/stg5e38e54f-ce14-4f33-8cfa-cdc6eb9b54b8/GCA_016708315.1_ASM1670831v1_genomic.fna.gz | prodigal -d GCA_016708315.1_ASM1670831v1_genomic.fna/cds.fna -a GCA_016708315.1_ASM1670831v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 12:52:22,836] [INFO] Task succeeded: Prodigal
[2023-06-30 12:52:22,836] [INFO] Task started: HMMsearch
[2023-06-30 12:52:22,836] [INFO] Running command: hmmsearch --tblout GCA_016708315.1_ASM1670831v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/reference_markers.hmm GCA_016708315.1_ASM1670831v1_genomic.fna/protein.faa > /dev/null
[2023-06-30 12:52:23,027] [INFO] Task succeeded: HMMsearch
[2023-06-30 12:52:23,028] [INFO] Found 6/6 markers.
[2023-06-30 12:52:23,059] [INFO] Query marker FASTA was written to GCA_016708315.1_ASM1670831v1_genomic.fna/markers.fasta
[2023-06-30 12:52:23,059] [INFO] Task started: Blastn
[2023-06-30 12:52:23,059] [INFO] Running command: blastn -query GCA_016708315.1_ASM1670831v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/reference_markers.fasta -out GCA_016708315.1_ASM1670831v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 12:52:23,682] [INFO] Task succeeded: Blastn
[2023-06-30 12:52:23,686] [INFO] Selected 24 target genomes.
[2023-06-30 12:52:23,687] [INFO] Target genome list was writen to GCA_016708315.1_ASM1670831v1_genomic.fna/target_genomes.txt
[2023-06-30 12:52:23,689] [INFO] Task started: fastANI
[2023-06-30 12:52:23,689] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e38e54f-ce14-4f33-8cfa-cdc6eb9b54b8/GCA_016708315.1_ASM1670831v1_genomic.fna.gz --refList GCA_016708315.1_ASM1670831v1_genomic.fna/target_genomes.txt --output GCA_016708315.1_ASM1670831v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 12:52:39,910] [INFO] Task succeeded: fastANI
[2023-06-30 12:52:39,910] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 12:52:39,910] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 12:52:39,912] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 12:52:39,912] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 12:52:39,912] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 12:52:39,914] [INFO] DFAST Taxonomy check result was written to GCA_016708315.1_ASM1670831v1_genomic.fna/tc_result.tsv
[2023-06-30 12:52:39,914] [INFO] ===== Taxonomy check completed =====
[2023-06-30 12:52:39,914] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 12:52:39,915] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/checkm_data
[2023-06-30 12:52:39,917] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 12:52:39,953] [INFO] Task started: CheckM
[2023-06-30 12:52:39,953] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_016708315.1_ASM1670831v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_016708315.1_ASM1670831v1_genomic.fna/checkm_input GCA_016708315.1_ASM1670831v1_genomic.fna/checkm_result
[2023-06-30 12:53:16,603] [INFO] Task succeeded: CheckM
[2023-06-30 12:53:16,604] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 12:53:16,622] [INFO] ===== Completeness check finished =====
[2023-06-30 12:53:16,622] [INFO] ===== Start GTDB Search =====
[2023-06-30 12:53:16,623] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_016708315.1_ASM1670831v1_genomic.fna/markers.fasta)
[2023-06-30 12:53:16,623] [INFO] Task started: Blastn
[2023-06-30 12:53:16,623] [INFO] Running command: blastn -query GCA_016708315.1_ASM1670831v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7b9220c9-f457-4f6d-b66f-d3b4999ed69d/dqc_reference/reference_markers_gtdb.fasta -out GCA_016708315.1_ASM1670831v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 12:53:17,607] [INFO] Task succeeded: Blastn
[2023-06-30 12:53:17,611] [INFO] Selected 21 target genomes.
[2023-06-30 12:53:17,611] [INFO] Target genome list was writen to GCA_016708315.1_ASM1670831v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 12:53:17,653] [INFO] Task started: fastANI
[2023-06-30 12:53:17,653] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e38e54f-ce14-4f33-8cfa-cdc6eb9b54b8/GCA_016708315.1_ASM1670831v1_genomic.fna.gz --refList GCA_016708315.1_ASM1670831v1_genomic.fna/target_genomes_gtdb.txt --output GCA_016708315.1_ASM1670831v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 12:53:31,362] [INFO] Task succeeded: fastANI
[2023-06-30 12:53:31,366] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 12:53:31,367] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_016708315.1	s__JADJGC01 sp016708315	100.0	1278	1278	d__Bacteria;p__Zixibacteria;c__MSB-5A5;o__CAIYYT01;f__CAIYYT01;g__JADJGC01	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_903903945.1	s__CAIYYT01 sp903903945	75.7003	59	1278	d__Bacteria;p__Zixibacteria;c__MSB-5A5;o__CAIYYT01;f__CAIYYT01;g__CAIYYT01	95.0	99.77	99.77	0.92	0.92	2	-
--------------------------------------------------------------------------------
[2023-06-30 12:53:31,369] [INFO] GTDB search result was written to GCA_016708315.1_ASM1670831v1_genomic.fna/result_gtdb.tsv
[2023-06-30 12:53:31,369] [INFO] ===== GTDB Search completed =====
[2023-06-30 12:53:31,372] [INFO] DFAST_QC result json was written to GCA_016708315.1_ASM1670831v1_genomic.fna/dqc_result.json
[2023-06-30 12:53:31,372] [INFO] DFAST_QC completed!
[2023-06-30 12:53:31,372] [INFO] Total running time: 0h1m22s