<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2020-05-14T00:00:00.000" last_update="2021-01-06T15:23:18.468" submission_date="2020-05-14T05:50:13.840" id="14918439" accession="SAMN14918439">   <Ids>     <Id db="BioSample" is_primary="1">SAMN14918439</Id>     <Id db_label="Sample name">BL5_bin.8</Id>     <Id db="SRA">SRS6658861</Id>   </Ids>   <Description>     <Title>MIMAG Metagenome-assembled Genome sample from Tepidibacillus decaturensis</Title>     <Organism taxonomy_id="1413211" taxonomy_name="Tepidibacillus decaturensis">       <OrganismName>Tepidibacillus decaturensis</OrganismName>     </Organism>     <Comment>       <Paragraph>Keywords: GSC:MIxS;MIMAG:6.0</Paragraph>     </Comment>   </Description>   <Owner>     <Name url="http://www.uni-bremen.de/en/microecophys.html">University of Bremen</Name>     <Contacts>       <Contact email="david.aromokeye@uni-bremen.de">         <Name>           <First>David</First>           <Last>Aromokeye</Last>           <Middle>Adeyemi</Middle>         </Name>       </Contact>     </Contacts>   </Owner>   <Models>     <Model>MIMAG</Model>   </Models>   <Package display_name="MIMAG: metagenome-assembled genome; version 6.0">MIMAG.6.0</Package>   <Attributes>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">marine sediment</Attribute>     <Attribute attribute_name="collection_date" harmonized_name="collection_date" display_name="collection date">Not applicable</Attribute>     <Attribute attribute_name="geo_loc_name" harmonized_name="geo_loc_name" display_name="geographic location">North Sea: German Bight (Helgoland Mud Area)</Attribute>     <Attribute attribute_name="lat_lon" harmonized_name="lat_lon" display_name="latitude and longitude">54.052300 N 7.580400 E</Attribute>     <Attribute attribute_name="rel_to_oxygen" harmonized_name="rel_to_oxygen" display_name="relationship to oxygen">obligate anaerobe</Attribute>     <Attribute attribute_name="samp_mat_process" harmonized_name="samp_mat_process" display_name="sample material processing">Enrichment was prepared using 5 mL volume from 4th transfer as starting material in a final volume of 50 mL widdel medium. Sample name identifies the unique information of each sample including the target 16S rRNA gene and the phase of the enrichment transfer.</Attribute>     <Attribute attribute_name="samp_size" harmonized_name="samp_size" display_name="sample size">5 mL enrichment was centrifuged down to 1 mL volume which was used for DNA extraction</Attribute>     <Attribute attribute_name="source_material_id" harmonized_name="source_material_id" display_name="source material identifiers">Benzoate + Lepidocrocite enrichment at 30ºC after the fifth transfer completely metabolised benzoate coupled to lepidocrocite reduction. 5mL was collected from the enrichment bottle and stored at -20ºC prior to DNA extraction and metagenome sequencing. Pooled DNA from three replicates of the BL5 enrichment used for metagenome sequencing. BL5_bin.8 is the reassembled metagenome assembled genome</Attribute>     <Attribute attribute_name="isolate" harmonized_name="isolate" display_name="isolate">BL5_bin.8</Attribute>     <Attribute attribute_name="metagenomic">1</Attribute>     <Attribute attribute_name="environmental-sample">1</Attribute>     <Attribute attribute_name="sample-type" harmonized_name="sample_type" display_name="sample type">metagenomic assembly</Attribute>     <Attribute attribute_name="metagenome-source">marine sediment metagenome</Attribute>     <Attribute attribute_name="env_broad_scale" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">missing</Attribute>     <Attribute attribute_name="env_local_scale" harmonized_name="env_local_scale" display_name="local-scale environmental context">missing</Attribute>     <Attribute attribute_name="env_medium" harmonized_name="env_medium" display_name="environmental medium">missing</Attribute>     <Attribute attribute_name="derived-from" harmonized_name="derived_from" display_name="derived from">This BioSample is a metagenomic assembly obtained from the marine sediment metagenome BioSamples: SAMN14820614, SAMN14820615, and SAMN14820616</Attribute>   </Attributes>   <Links>     <Link type="entrez" target="bioproject" label="PRJNA630030">630030</Link>   </Links>   <Status status="live" when="2020-05-14T05:50:13.841"/> </BioSample> </BioSampleSet>
