[2023-06-18 21:39:24,951] [INFO] DFAST_QC pipeline started.
[2023-06-18 21:39:24,953] [INFO] DFAST_QC version: 0.5.7
[2023-06-18 21:39:24,953] [INFO] DQC Reference Directory: /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference
[2023-06-18 21:39:26,166] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-18 21:39:26,167] [INFO] Task started: Prodigal
[2023-06-18 21:39:26,167] [INFO] Running command: gunzip -c /var/lib/cwl/stg15ca3eac-0ff1-406b-83f1-bc5e0e26b16f/GCA_018401015.1_ASM1840101v1_genomic.fna.gz | prodigal -d GCA_018401015.1_ASM1840101v1_genomic.fna/cds.fna -a GCA_018401015.1_ASM1840101v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-18 21:39:38,953] [INFO] Task succeeded: Prodigal
[2023-06-18 21:39:38,954] [INFO] Task started: HMMsearch
[2023-06-18 21:39:38,954] [INFO] Running command: hmmsearch --tblout GCA_018401015.1_ASM1840101v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/reference_markers.hmm GCA_018401015.1_ASM1840101v1_genomic.fna/protein.faa > /dev/null
[2023-06-18 21:39:39,268] [INFO] Task succeeded: HMMsearch
[2023-06-18 21:39:39,270] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg15ca3eac-0ff1-406b-83f1-bc5e0e26b16f/GCA_018401015.1_ASM1840101v1_genomic.fna.gz]
[2023-06-18 21:39:39,304] [INFO] Query marker FASTA was written to GCA_018401015.1_ASM1840101v1_genomic.fna/markers.fasta
[2023-06-18 21:39:39,305] [INFO] Task started: Blastn
[2023-06-18 21:39:39,305] [INFO] Running command: blastn -query GCA_018401015.1_ASM1840101v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/reference_markers.fasta -out GCA_018401015.1_ASM1840101v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 21:39:39,987] [INFO] Task succeeded: Blastn
[2023-06-18 21:39:39,990] [INFO] Selected 20 target genomes.
[2023-06-18 21:39:39,990] [INFO] Target genome list was writen to GCA_018401015.1_ASM1840101v1_genomic.fna/target_genomes.txt
[2023-06-18 21:39:39,993] [INFO] Task started: fastANI
[2023-06-18 21:39:39,993] [INFO] Running command: fastANI --query /var/lib/cwl/stg15ca3eac-0ff1-406b-83f1-bc5e0e26b16f/GCA_018401015.1_ASM1840101v1_genomic.fna.gz --refList GCA_018401015.1_ASM1840101v1_genomic.fna/target_genomes.txt --output GCA_018401015.1_ASM1840101v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-18 21:39:53,816] [INFO] Task succeeded: fastANI
[2023-06-18 21:39:53,817] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-18 21:39:53,817] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-18 21:39:53,821] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-18 21:39:53,821] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-18 21:39:53,821] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-18 21:39:53,826] [INFO] DFAST Taxonomy check result was written to GCA_018401015.1_ASM1840101v1_genomic.fna/tc_result.tsv
[2023-06-18 21:39:53,827] [INFO] ===== Taxonomy check completed =====
[2023-06-18 21:39:53,827] [INFO] ===== Start completeness check using CheckM =====
[2023-06-18 21:39:53,827] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/checkm_data
[2023-06-18 21:39:53,830] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-18 21:39:53,862] [INFO] Task started: CheckM
[2023-06-18 21:39:53,863] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018401015.1_ASM1840101v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018401015.1_ASM1840101v1_genomic.fna/checkm_input GCA_018401015.1_ASM1840101v1_genomic.fna/checkm_result
[2023-06-18 21:40:33,953] [INFO] Task succeeded: CheckM
[2023-06-18 21:40:33,954] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-18 21:40:33,980] [INFO] ===== Completeness check finished =====
[2023-06-18 21:40:33,980] [INFO] ===== Start GTDB Search =====
[2023-06-18 21:40:33,981] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018401015.1_ASM1840101v1_genomic.fna/markers.fasta)
[2023-06-18 21:40:33,981] [INFO] Task started: Blastn
[2023-06-18 21:40:33,981] [INFO] Running command: blastn -query GCA_018401015.1_ASM1840101v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg60658a4e-a4db-43e6-b6ca-0073d5907a65/dqc_reference/reference_markers_gtdb.fasta -out GCA_018401015.1_ASM1840101v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 21:40:34,925] [INFO] Task succeeded: Blastn
[2023-06-18 21:40:34,930] [INFO] Selected 22 target genomes.
[2023-06-18 21:40:34,930] [INFO] Target genome list was writen to GCA_018401015.1_ASM1840101v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-18 21:40:34,938] [INFO] Task started: fastANI
[2023-06-18 21:40:34,938] [INFO] Running command: fastANI --query /var/lib/cwl/stg15ca3eac-0ff1-406b-83f1-bc5e0e26b16f/GCA_018401015.1_ASM1840101v1_genomic.fna.gz --refList GCA_018401015.1_ASM1840101v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018401015.1_ASM1840101v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-18 21:40:45,722] [INFO] Task succeeded: fastANI
[2023-06-18 21:40:45,727] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-18 21:40:45,727] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018401015.1	s__M50B96 sp018401015	100.0	910	917	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__M50B96;g__M50B96	95.0	99.83	99.83	0.98	0.98	2	conclusive
--------------------------------------------------------------------------------
[2023-06-18 21:40:45,729] [INFO] GTDB search result was written to GCA_018401015.1_ASM1840101v1_genomic.fna/result_gtdb.tsv
[2023-06-18 21:40:45,730] [INFO] ===== GTDB Search completed =====
[2023-06-18 21:40:45,734] [INFO] DFAST_QC result json was written to GCA_018401015.1_ASM1840101v1_genomic.fna/dqc_result.json
[2023-06-18 21:40:45,735] [INFO] DFAST_QC completed!
[2023-06-18 21:40:45,735] [INFO] Total running time: 0h1m21s