[2023-06-18 14:23:41,995] [INFO] DFAST_QC pipeline started.
[2023-06-18 14:23:41,997] [INFO] DFAST_QC version: 0.5.7
[2023-06-18 14:23:41,997] [INFO] DQC Reference Directory: /var/lib/cwl/stg61502c13-cab1-4d0c-aae1-d4898ed7bd0c/dqc_reference
[2023-06-18 14:23:43,243] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-18 14:23:43,244] [INFO] Task started: Prodigal
[2023-06-18 14:23:43,244] [INFO] Running command: gunzip -c /var/lib/cwl/stg026d78aa-bfc7-4d90-a76f-9dcb0ef0186f/GCA_018609855.1_ASM1860985v1_genomic.fna.gz | prodigal -d GCA_018609855.1_ASM1860985v1_genomic.fna/cds.fna -a GCA_018609855.1_ASM1860985v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-18 14:23:44,703] [INFO] Task succeeded: Prodigal
[2023-06-18 14:23:44,704] [INFO] Task started: HMMsearch
[2023-06-18 14:23:44,704] [INFO] Running command: hmmsearch --tblout GCA_018609855.1_ASM1860985v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg61502c13-cab1-4d0c-aae1-d4898ed7bd0c/dqc_reference/reference_markers.hmm GCA_018609855.1_ASM1860985v1_genomic.fna/protein.faa > /dev/null
[2023-06-18 14:23:44,967] [INFO] Task succeeded: HMMsearch
[2023-06-18 14:23:44,969] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg026d78aa-bfc7-4d90-a76f-9dcb0ef0186f/GCA_018609855.1_ASM1860985v1_genomic.fna.gz]
[2023-06-18 14:23:44,982] [INFO] Query marker FASTA was written to GCA_018609855.1_ASM1860985v1_genomic.fna/markers.fasta
[2023-06-18 14:23:44,982] [INFO] Task started: Blastn
[2023-06-18 14:23:44,983] [INFO] Running command: blastn -query GCA_018609855.1_ASM1860985v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg61502c13-cab1-4d0c-aae1-d4898ed7bd0c/dqc_reference/reference_markers.fasta -out GCA_018609855.1_ASM1860985v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 14:23:45,470] [INFO] Task succeeded: Blastn
[2023-06-18 14:23:45,474] [INFO] Selected 0 target genomes.
[2023-06-18 14:23:45,475] [INFO] Target genome list was writen to GCA_018609855.1_ASM1860985v1_genomic.fna/target_genomes.txt
[2023-06-18 14:23:45,475] [ERROR] File is empty. [GCA_018609855.1_ASM1860985v1_genomic.fna/target_genomes.txt]
[2023-06-18 14:23:45,475] [ERROR] Task failed. No target genome found.
[2023-06-18 14:23:45,476] [INFO] ===== Start completeness check using CheckM =====
[2023-06-18 14:23:45,476] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg61502c13-cab1-4d0c-aae1-d4898ed7bd0c/dqc_reference/checkm_data
[2023-06-18 14:23:45,481] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-18 14:23:45,495] [INFO] Task started: CheckM
[2023-06-18 14:23:45,496] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018609855.1_ASM1860985v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018609855.1_ASM1860985v1_genomic.fna/checkm_input GCA_018609855.1_ASM1860985v1_genomic.fna/checkm_result
[2023-06-18 14:23:58,234] [INFO] Task succeeded: CheckM
[2023-06-18 14:23:58,235] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 65.81%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-18 14:23:58,254] [INFO] ===== Completeness check finished =====
[2023-06-18 14:23:58,255] [INFO] ===== Start GTDB Search =====
[2023-06-18 14:23:58,255] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018609855.1_ASM1860985v1_genomic.fna/markers.fasta)
[2023-06-18 14:23:58,255] [INFO] Task started: Blastn
[2023-06-18 14:23:58,255] [INFO] Running command: blastn -query GCA_018609855.1_ASM1860985v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg61502c13-cab1-4d0c-aae1-d4898ed7bd0c/dqc_reference/reference_markers_gtdb.fasta -out GCA_018609855.1_ASM1860985v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 14:23:58,772] [INFO] Task succeeded: Blastn
[2023-06-18 14:23:58,779] [INFO] Selected 3 target genomes.
[2023-06-18 14:23:58,779] [INFO] Target genome list was writen to GCA_018609855.1_ASM1860985v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-18 14:23:58,818] [INFO] Task started: fastANI
[2023-06-18 14:23:58,818] [INFO] Running command: fastANI --query /var/lib/cwl/stg026d78aa-bfc7-4d90-a76f-9dcb0ef0186f/GCA_018609855.1_ASM1860985v1_genomic.fna.gz --refList GCA_018609855.1_ASM1860985v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018609855.1_ASM1860985v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-18 14:23:59,519] [INFO] Task succeeded: fastANI
[2023-06-18 14:23:59,524] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-18 14:23:59,524] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018609855.1	s__JAHENU01 sp018609855	100.0	172	177	d__Archaea;p__Nanohaloarchaeota;c__Nanosalinia;o__Nanosalinales;f__JAHENU01;g__JAHENU01	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-18 14:23:59,526] [INFO] GTDB search result was written to GCA_018609855.1_ASM1860985v1_genomic.fna/result_gtdb.tsv
[2023-06-18 14:23:59,527] [INFO] ===== GTDB Search completed =====
[2023-06-18 14:23:59,529] [INFO] DFAST_QC result json was written to GCA_018609855.1_ASM1860985v1_genomic.fna/dqc_result.json
[2023-06-18 14:23:59,529] [INFO] DFAST_QC completed!
[2023-06-18 14:23:59,529] [INFO] Total running time: 0h0m18s