[2023-06-19 07:50:09,409] [INFO] DFAST_QC pipeline started.
[2023-06-19 07:50:09,412] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 07:50:09,412] [INFO] DQC Reference Directory: /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference
[2023-06-19 07:50:10,696] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 07:50:10,697] [INFO] Task started: Prodigal
[2023-06-19 07:50:10,698] [INFO] Running command: gunzip -c /var/lib/cwl/stgb9020943-9f1d-4344-9125-0a53728767da/GCA_018652945.1_ASM1865294v1_genomic.fna.gz | prodigal -d GCA_018652945.1_ASM1865294v1_genomic.fna/cds.fna -a GCA_018652945.1_ASM1865294v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 07:50:31,989] [INFO] Task succeeded: Prodigal
[2023-06-19 07:50:31,989] [INFO] Task started: HMMsearch
[2023-06-19 07:50:31,990] [INFO] Running command: hmmsearch --tblout GCA_018652945.1_ASM1865294v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/reference_markers.hmm GCA_018652945.1_ASM1865294v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 07:50:32,292] [INFO] Task succeeded: HMMsearch
[2023-06-19 07:50:32,293] [INFO] Found 6/6 markers.
[2023-06-19 07:50:32,339] [INFO] Query marker FASTA was written to GCA_018652945.1_ASM1865294v1_genomic.fna/markers.fasta
[2023-06-19 07:50:32,340] [INFO] Task started: Blastn
[2023-06-19 07:50:32,340] [INFO] Running command: blastn -query GCA_018652945.1_ASM1865294v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/reference_markers.fasta -out GCA_018652945.1_ASM1865294v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 07:50:32,950] [INFO] Task succeeded: Blastn
[2023-06-19 07:50:32,957] [INFO] Selected 25 target genomes.
[2023-06-19 07:50:32,957] [INFO] Target genome list was writen to GCA_018652945.1_ASM1865294v1_genomic.fna/target_genomes.txt
[2023-06-19 07:50:32,987] [INFO] Task started: fastANI
[2023-06-19 07:50:32,988] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9020943-9f1d-4344-9125-0a53728767da/GCA_018652945.1_ASM1865294v1_genomic.fna.gz --refList GCA_018652945.1_ASM1865294v1_genomic.fna/target_genomes.txt --output GCA_018652945.1_ASM1865294v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 07:50:47,910] [INFO] Task succeeded: fastANI
[2023-06-19 07:50:47,911] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 07:50:47,911] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 07:50:47,913] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 07:50:47,913] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 07:50:47,914] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 07:50:47,915] [INFO] DFAST Taxonomy check result was written to GCA_018652945.1_ASM1865294v1_genomic.fna/tc_result.tsv
[2023-06-19 07:50:47,916] [INFO] ===== Taxonomy check completed =====
[2023-06-19 07:50:47,916] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 07:50:47,916] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/checkm_data
[2023-06-19 07:50:47,920] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 07:50:47,973] [INFO] Task started: CheckM
[2023-06-19 07:50:47,973] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018652945.1_ASM1865294v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018652945.1_ASM1865294v1_genomic.fna/checkm_input GCA_018652945.1_ASM1865294v1_genomic.fna/checkm_result
[2023-06-19 07:51:52,363] [INFO] Task succeeded: CheckM
[2023-06-19 07:51:52,365] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 78.12%
Contamintation: 11.57%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 07:51:52,391] [INFO] ===== Completeness check finished =====
[2023-06-19 07:51:52,391] [INFO] ===== Start GTDB Search =====
[2023-06-19 07:51:52,392] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018652945.1_ASM1865294v1_genomic.fna/markers.fasta)
[2023-06-19 07:51:52,392] [INFO] Task started: Blastn
[2023-06-19 07:51:52,392] [INFO] Running command: blastn -query GCA_018652945.1_ASM1865294v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcfc10d7f-3eea-4285-a33b-b80108b978cb/dqc_reference/reference_markers_gtdb.fasta -out GCA_018652945.1_ASM1865294v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 07:51:53,147] [INFO] Task succeeded: Blastn
[2023-06-19 07:51:53,152] [INFO] Selected 28 target genomes.
[2023-06-19 07:51:53,153] [INFO] Target genome list was writen to GCA_018652945.1_ASM1865294v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 07:51:53,164] [INFO] Task started: fastANI
[2023-06-19 07:51:53,165] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9020943-9f1d-4344-9125-0a53728767da/GCA_018652945.1_ASM1865294v1_genomic.fna.gz --refList GCA_018652945.1_ASM1865294v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018652945.1_ASM1865294v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 07:52:08,642] [INFO] Task succeeded: fastANI
[2023-06-19 07:52:08,647] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 07:52:08,648] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018669475.1	s__JABHCC01 sp018669475	99.8114	1413	1541	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA12481;g__JABHCC01	95.0	99.78	99.71	0.92	0.88	7	conclusive
--------------------------------------------------------------------------------
[2023-06-19 07:52:08,650] [INFO] GTDB search result was written to GCA_018652945.1_ASM1865294v1_genomic.fna/result_gtdb.tsv
[2023-06-19 07:52:08,650] [INFO] ===== GTDB Search completed =====
[2023-06-19 07:52:08,652] [INFO] DFAST_QC result json was written to GCA_018652945.1_ASM1865294v1_genomic.fna/dqc_result.json
[2023-06-19 07:52:08,653] [INFO] DFAST_QC completed!
[2023-06-19 07:52:08,653] [INFO] Total running time: 0h1m59s