[2023-06-19 14:15:59,203] [INFO] DFAST_QC pipeline started.
[2023-06-19 14:15:59,206] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 14:15:59,206] [INFO] DQC Reference Directory: /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference
[2023-06-19 14:16:00,658] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 14:16:00,659] [INFO] Task started: Prodigal
[2023-06-19 14:16:00,659] [INFO] Running command: gunzip -c /var/lib/cwl/stga8b8ff15-f98e-4226-9c8b-b52e8a9ca313/GCA_018655505.1_ASM1865550v1_genomic.fna.gz | prodigal -d GCA_018655505.1_ASM1865550v1_genomic.fna/cds.fna -a GCA_018655505.1_ASM1865550v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 14:16:04,555] [INFO] Task succeeded: Prodigal
[2023-06-19 14:16:04,556] [INFO] Task started: HMMsearch
[2023-06-19 14:16:04,556] [INFO] Running command: hmmsearch --tblout GCA_018655505.1_ASM1865550v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/reference_markers.hmm GCA_018655505.1_ASM1865550v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 14:16:04,789] [INFO] Task succeeded: HMMsearch
[2023-06-19 14:16:04,792] [INFO] Found 6/6 markers.
[2023-06-19 14:16:04,807] [INFO] Query marker FASTA was written to GCA_018655505.1_ASM1865550v1_genomic.fna/markers.fasta
[2023-06-19 14:16:04,808] [INFO] Task started: Blastn
[2023-06-19 14:16:04,808] [INFO] Running command: blastn -query GCA_018655505.1_ASM1865550v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/reference_markers.fasta -out GCA_018655505.1_ASM1865550v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 14:16:05,397] [INFO] Task succeeded: Blastn
[2023-06-19 14:16:05,402] [INFO] Selected 10 target genomes.
[2023-06-19 14:16:05,402] [INFO] Target genome list was writen to GCA_018655505.1_ASM1865550v1_genomic.fna/target_genomes.txt
[2023-06-19 14:16:05,405] [INFO] Task started: fastANI
[2023-06-19 14:16:05,405] [INFO] Running command: fastANI --query /var/lib/cwl/stga8b8ff15-f98e-4226-9c8b-b52e8a9ca313/GCA_018655505.1_ASM1865550v1_genomic.fna.gz --refList GCA_018655505.1_ASM1865550v1_genomic.fna/target_genomes.txt --output GCA_018655505.1_ASM1865550v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 14:16:08,669] [INFO] Task succeeded: fastANI
[2023-06-19 14:16:08,670] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 14:16:08,670] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 14:16:08,672] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 14:16:08,672] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 14:16:08,672] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 14:16:08,674] [INFO] DFAST Taxonomy check result was written to GCA_018655505.1_ASM1865550v1_genomic.fna/tc_result.tsv
[2023-06-19 14:16:08,674] [INFO] ===== Taxonomy check completed =====
[2023-06-19 14:16:08,674] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 14:16:08,674] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/checkm_data
[2023-06-19 14:16:08,677] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 14:16:08,692] [INFO] Task started: CheckM
[2023-06-19 14:16:08,692] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018655505.1_ASM1865550v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018655505.1_ASM1865550v1_genomic.fna/checkm_input GCA_018655505.1_ASM1865550v1_genomic.fna/checkm_result
[2023-06-19 14:16:28,051] [INFO] Task succeeded: CheckM
[2023-06-19 14:16:28,052] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.50%
Contamintation: 1.04%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 14:16:28,080] [INFO] ===== Completeness check finished =====
[2023-06-19 14:16:28,080] [INFO] ===== Start GTDB Search =====
[2023-06-19 14:16:28,081] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018655505.1_ASM1865550v1_genomic.fna/markers.fasta)
[2023-06-19 14:16:28,081] [INFO] Task started: Blastn
[2023-06-19 14:16:28,081] [INFO] Running command: blastn -query GCA_018655505.1_ASM1865550v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3944fd06-df60-463b-ab56-e825e57ee284/dqc_reference/reference_markers_gtdb.fasta -out GCA_018655505.1_ASM1865550v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 14:16:28,900] [INFO] Task succeeded: Blastn
[2023-06-19 14:16:28,904] [INFO] Selected 17 target genomes.
[2023-06-19 14:16:28,904] [INFO] Target genome list was writen to GCA_018655505.1_ASM1865550v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 14:16:28,907] [INFO] Task started: fastANI
[2023-06-19 14:16:28,907] [INFO] Running command: fastANI --query /var/lib/cwl/stga8b8ff15-f98e-4226-9c8b-b52e8a9ca313/GCA_018655505.1_ASM1865550v1_genomic.fna.gz --refList GCA_018655505.1_ASM1865550v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018655505.1_ASM1865550v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 14:16:33,504] [INFO] Task succeeded: fastANI
[2023-06-19 14:16:33,507] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 14:16:33,507] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018652265.1	s__JABICL01 sp018652265	99.9095	290	303	d__Bacteria;p__Patescibacteria;c__Gracilibacteria;o__Peribacterales;f__JABICL01;g__JABICL01	95.0	99.93	99.91	0.96	0.94	4	conclusive
--------------------------------------------------------------------------------
[2023-06-19 14:16:33,511] [INFO] GTDB search result was written to GCA_018655505.1_ASM1865550v1_genomic.fna/result_gtdb.tsv
[2023-06-19 14:16:33,512] [INFO] ===== GTDB Search completed =====
[2023-06-19 14:16:33,515] [INFO] DFAST_QC result json was written to GCA_018655505.1_ASM1865550v1_genomic.fna/dqc_result.json
[2023-06-19 14:16:33,515] [INFO] DFAST_QC completed!
[2023-06-19 14:16:33,515] [INFO] Total running time: 0h0m34s