[2023-06-19 02:25:25,492] [INFO] DFAST_QC pipeline started.
[2023-06-19 02:25:25,502] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 02:25:25,502] [INFO] DQC Reference Directory: /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference
[2023-06-19 02:25:28,474] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 02:25:28,475] [INFO] Task started: Prodigal
[2023-06-19 02:25:28,475] [INFO] Running command: gunzip -c /var/lib/cwl/stg9d5a571d-04bb-4aec-97da-27a8c9d5826c/GCA_018656645.1_ASM1865664v1_genomic.fna.gz | prodigal -d GCA_018656645.1_ASM1865664v1_genomic.fna/cds.fna -a GCA_018656645.1_ASM1865664v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 02:25:33,870] [INFO] Task succeeded: Prodigal
[2023-06-19 02:25:33,870] [INFO] Task started: HMMsearch
[2023-06-19 02:25:33,871] [INFO] Running command: hmmsearch --tblout GCA_018656645.1_ASM1865664v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/reference_markers.hmm GCA_018656645.1_ASM1865664v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 02:25:34,059] [INFO] Task succeeded: HMMsearch
[2023-06-19 02:25:34,060] [INFO] Found 6/6 markers.
[2023-06-19 02:25:34,081] [INFO] Query marker FASTA was written to GCA_018656645.1_ASM1865664v1_genomic.fna/markers.fasta
[2023-06-19 02:25:34,081] [INFO] Task started: Blastn
[2023-06-19 02:25:34,081] [INFO] Running command: blastn -query GCA_018656645.1_ASM1865664v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/reference_markers.fasta -out GCA_018656645.1_ASM1865664v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 02:25:34,635] [INFO] Task succeeded: Blastn
[2023-06-19 02:25:34,639] [INFO] Selected 13 target genomes.
[2023-06-19 02:25:34,639] [INFO] Target genome list was writen to GCA_018656645.1_ASM1865664v1_genomic.fna/target_genomes.txt
[2023-06-19 02:25:34,640] [INFO] Task started: fastANI
[2023-06-19 02:25:34,640] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d5a571d-04bb-4aec-97da-27a8c9d5826c/GCA_018656645.1_ASM1865664v1_genomic.fna.gz --refList GCA_018656645.1_ASM1865664v1_genomic.fna/target_genomes.txt --output GCA_018656645.1_ASM1865664v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 02:25:40,275] [INFO] Task succeeded: fastANI
[2023-06-19 02:25:40,276] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 02:25:40,276] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 02:25:40,278] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 02:25:40,278] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 02:25:40,278] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 02:25:40,279] [INFO] DFAST Taxonomy check result was written to GCA_018656645.1_ASM1865664v1_genomic.fna/tc_result.tsv
[2023-06-19 02:25:40,280] [INFO] ===== Taxonomy check completed =====
[2023-06-19 02:25:40,280] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 02:25:40,280] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/checkm_data
[2023-06-19 02:25:40,283] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 02:25:40,304] [INFO] Task started: CheckM
[2023-06-19 02:25:40,304] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018656645.1_ASM1865664v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018656645.1_ASM1865664v1_genomic.fna/checkm_input GCA_018656645.1_ASM1865664v1_genomic.fna/checkm_result
[2023-06-19 02:26:02,306] [INFO] Task succeeded: CheckM
[2023-06-19 02:26:02,307] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 02:26:02,321] [INFO] ===== Completeness check finished =====
[2023-06-19 02:26:02,321] [INFO] ===== Start GTDB Search =====
[2023-06-19 02:26:02,321] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018656645.1_ASM1865664v1_genomic.fna/markers.fasta)
[2023-06-19 02:26:02,322] [INFO] Task started: Blastn
[2023-06-19 02:26:02,322] [INFO] Running command: blastn -query GCA_018656645.1_ASM1865664v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd1dab751-b883-437d-b222-19894a38be99/dqc_reference/reference_markers_gtdb.fasta -out GCA_018656645.1_ASM1865664v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 02:26:03,111] [INFO] Task succeeded: Blastn
[2023-06-19 02:26:03,114] [INFO] Selected 14 target genomes.
[2023-06-19 02:26:03,114] [INFO] Target genome list was writen to GCA_018656645.1_ASM1865664v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 02:26:03,174] [INFO] Task started: fastANI
[2023-06-19 02:26:03,174] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d5a571d-04bb-4aec-97da-27a8c9d5826c/GCA_018656645.1_ASM1865664v1_genomic.fna.gz --refList GCA_018656645.1_ASM1865664v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018656645.1_ASM1865664v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 02:26:08,163] [INFO] Task succeeded: fastANI
[2023-06-19 02:26:08,171] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 02:26:08,171] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018656645.1	s__GCA-002718515 sp018656645	100.0	589	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_014238125.1	s__GCA-002718515 sp014238125	81.7457	365	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014381305.1	s__GCA-002718515 sp014381305	80.1195	307	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018657585.1	s__GCA-002718515 sp018657585	78.2532	88	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018701395.1	s__GCA-002718515 sp018701395	77.683	89	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	99.63	99.41	0.94	0.92	4	-
GCA_014238785.1	s__GCA-002718515 sp014238785	77.0874	84	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_012965315.1	s__GCA-002718515 sp012965315	76.7095	67	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	99.71	99.67	0.87	0.85	4	-
GCA_018665385.1	s__GCA-002718515 sp018665385	76.6487	57	597	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	99.09	99.03	0.85	0.83	4	-
--------------------------------------------------------------------------------
[2023-06-19 02:26:08,173] [INFO] GTDB search result was written to GCA_018656645.1_ASM1865664v1_genomic.fna/result_gtdb.tsv
[2023-06-19 02:26:08,173] [INFO] ===== GTDB Search completed =====
[2023-06-19 02:26:08,175] [INFO] DFAST_QC result json was written to GCA_018656645.1_ASM1865664v1_genomic.fna/dqc_result.json
[2023-06-19 02:26:08,176] [INFO] DFAST_QC completed!
[2023-06-19 02:26:08,176] [INFO] Total running time: 0h0m43s