[2023-06-18 19:52:04,970] [INFO] DFAST_QC pipeline started.
[2023-06-18 19:52:04,972] [INFO] DFAST_QC version: 0.5.7
[2023-06-18 19:52:04,972] [INFO] DQC Reference Directory: /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference
[2023-06-18 19:52:06,509] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-18 19:52:06,510] [INFO] Task started: Prodigal
[2023-06-18 19:52:06,510] [INFO] Running command: gunzip -c /var/lib/cwl/stgbb9d979b-862b-418f-b2ff-9a4c758bb152/GCA_018671035.1_ASM1867103v1_genomic.fna.gz | prodigal -d GCA_018671035.1_ASM1867103v1_genomic.fna/cds.fna -a GCA_018671035.1_ASM1867103v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-18 19:52:09,018] [INFO] Task succeeded: Prodigal
[2023-06-18 19:52:09,019] [INFO] Task started: HMMsearch
[2023-06-18 19:52:09,019] [INFO] Running command: hmmsearch --tblout GCA_018671035.1_ASM1867103v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/reference_markers.hmm GCA_018671035.1_ASM1867103v1_genomic.fna/protein.faa > /dev/null
[2023-06-18 19:52:09,249] [INFO] Task succeeded: HMMsearch
[2023-06-18 19:52:09,251] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgbb9d979b-862b-418f-b2ff-9a4c758bb152/GCA_018671035.1_ASM1867103v1_genomic.fna.gz]
[2023-06-18 19:52:09,271] [INFO] Query marker FASTA was written to GCA_018671035.1_ASM1867103v1_genomic.fna/markers.fasta
[2023-06-18 19:52:09,272] [INFO] Task started: Blastn
[2023-06-18 19:52:09,272] [INFO] Running command: blastn -query GCA_018671035.1_ASM1867103v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/reference_markers.fasta -out GCA_018671035.1_ASM1867103v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 19:52:09,823] [INFO] Task succeeded: Blastn
[2023-06-18 19:52:09,827] [INFO] Selected 5 target genomes.
[2023-06-18 19:52:09,827] [INFO] Target genome list was writen to GCA_018671035.1_ASM1867103v1_genomic.fna/target_genomes.txt
[2023-06-18 19:52:09,829] [INFO] Task started: fastANI
[2023-06-18 19:52:09,830] [INFO] Running command: fastANI --query /var/lib/cwl/stgbb9d979b-862b-418f-b2ff-9a4c758bb152/GCA_018671035.1_ASM1867103v1_genomic.fna.gz --refList GCA_018671035.1_ASM1867103v1_genomic.fna/target_genomes.txt --output GCA_018671035.1_ASM1867103v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-18 19:52:11,638] [INFO] Task succeeded: fastANI
[2023-06-18 19:52:11,639] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-18 19:52:11,639] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-18 19:52:11,640] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-18 19:52:11,641] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-18 19:52:11,641] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-18 19:52:11,643] [INFO] DFAST Taxonomy check result was written to GCA_018671035.1_ASM1867103v1_genomic.fna/tc_result.tsv
[2023-06-18 19:52:11,643] [INFO] ===== Taxonomy check completed =====
[2023-06-18 19:52:11,643] [INFO] ===== Start completeness check using CheckM =====
[2023-06-18 19:52:11,643] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/checkm_data
[2023-06-18 19:52:11,646] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-18 19:52:11,664] [INFO] Task started: CheckM
[2023-06-18 19:52:11,664] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018671035.1_ASM1867103v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018671035.1_ASM1867103v1_genomic.fna/checkm_input GCA_018671035.1_ASM1867103v1_genomic.fna/checkm_result
[2023-06-18 19:52:27,903] [INFO] Task succeeded: CheckM
[2023-06-18 19:52:27,904] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.94%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-18 19:52:27,926] [INFO] ===== Completeness check finished =====
[2023-06-18 19:52:27,927] [INFO] ===== Start GTDB Search =====
[2023-06-18 19:52:27,927] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018671035.1_ASM1867103v1_genomic.fna/markers.fasta)
[2023-06-18 19:52:27,928] [INFO] Task started: Blastn
[2023-06-18 19:52:27,928] [INFO] Running command: blastn -query GCA_018671035.1_ASM1867103v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg988f80f2-b732-4570-961c-7fc114d6b49d/dqc_reference/reference_markers_gtdb.fasta -out GCA_018671035.1_ASM1867103v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 19:52:28,389] [INFO] Task succeeded: Blastn
[2023-06-18 19:52:28,393] [INFO] Selected 7 target genomes.
[2023-06-18 19:52:28,393] [INFO] Target genome list was writen to GCA_018671035.1_ASM1867103v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-18 19:52:28,399] [INFO] Task started: fastANI
[2023-06-18 19:52:28,399] [INFO] Running command: fastANI --query /var/lib/cwl/stgbb9d979b-862b-418f-b2ff-9a4c758bb152/GCA_018671035.1_ASM1867103v1_genomic.fna.gz --refList GCA_018671035.1_ASM1867103v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018671035.1_ASM1867103v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-18 19:52:30,520] [INFO] Task succeeded: fastANI
[2023-06-18 19:52:30,527] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-18 19:52:30,527] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002494975.1	s__MGIIb-O2 sp002494975	98.5001	326	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	98.81	97.76	0.81	0.69	24	conclusive
GCA_902511005.1	s__MGIIb-O2 sp902511005	91.4336	318	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	98.72	98.72	0.78	0.78	2	-
GCA_902514485.1	s__MGIIb-O2 sp902514485	88.9248	211	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	98.21	98.21	0.74	0.74	2	-
GCA_016777225.1	s__MGIIb-O2 sp016777225	81.1135	312	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002495525.1	s__MGIIb-O2 sp002495525	78.5994	179	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	98.21	96.68	0.79	0.75	3	-
GCA_002504905.1	s__MGIIb-O2 sp002504905	78.106	181	410	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	99.85	99.68	0.95	0.91	6	-
--------------------------------------------------------------------------------
[2023-06-18 19:52:30,529] [INFO] GTDB search result was written to GCA_018671035.1_ASM1867103v1_genomic.fna/result_gtdb.tsv
[2023-06-18 19:52:30,530] [INFO] ===== GTDB Search completed =====
[2023-06-18 19:52:30,532] [INFO] DFAST_QC result json was written to GCA_018671035.1_ASM1867103v1_genomic.fna/dqc_result.json
[2023-06-18 19:52:30,532] [INFO] DFAST_QC completed!
[2023-06-18 19:52:30,533] [INFO] Total running time: 0h0m26s