[2023-06-19 11:45:58,640] [INFO] DFAST_QC pipeline started.
[2023-06-19 11:45:58,644] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 11:45:58,644] [INFO] DQC Reference Directory: /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference
[2023-06-19 11:46:00,063] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 11:46:00,064] [INFO] Task started: Prodigal
[2023-06-19 11:46:00,064] [INFO] Running command: gunzip -c /var/lib/cwl/stg41cb9e8b-ce49-4314-b6c0-edba9b98fdaf/GCA_018699085.1_ASM1869908v1_genomic.fna.gz | prodigal -d GCA_018699085.1_ASM1869908v1_genomic.fna/cds.fna -a GCA_018699085.1_ASM1869908v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 11:46:08,832] [INFO] Task succeeded: Prodigal
[2023-06-19 11:46:08,832] [INFO] Task started: HMMsearch
[2023-06-19 11:46:08,832] [INFO] Running command: hmmsearch --tblout GCA_018699085.1_ASM1869908v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/reference_markers.hmm GCA_018699085.1_ASM1869908v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 11:46:09,035] [INFO] Task succeeded: HMMsearch
[2023-06-19 11:46:09,036] [INFO] Found 6/6 markers.
[2023-06-19 11:46:09,064] [INFO] Query marker FASTA was written to GCA_018699085.1_ASM1869908v1_genomic.fna/markers.fasta
[2023-06-19 11:46:09,064] [INFO] Task started: Blastn
[2023-06-19 11:46:09,064] [INFO] Running command: blastn -query GCA_018699085.1_ASM1869908v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/reference_markers.fasta -out GCA_018699085.1_ASM1869908v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 11:46:09,683] [INFO] Task succeeded: Blastn
[2023-06-19 11:46:09,703] [INFO] Selected 33 target genomes.
[2023-06-19 11:46:09,703] [INFO] Target genome list was writen to GCA_018699085.1_ASM1869908v1_genomic.fna/target_genomes.txt
[2023-06-19 11:46:09,707] [INFO] Task started: fastANI
[2023-06-19 11:46:09,707] [INFO] Running command: fastANI --query /var/lib/cwl/stg41cb9e8b-ce49-4314-b6c0-edba9b98fdaf/GCA_018699085.1_ASM1869908v1_genomic.fna.gz --refList GCA_018699085.1_ASM1869908v1_genomic.fna/target_genomes.txt --output GCA_018699085.1_ASM1869908v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 11:46:33,079] [INFO] Task succeeded: fastANI
[2023-06-19 11:46:33,079] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 11:46:33,080] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 11:46:33,081] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 11:46:33,081] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 11:46:33,082] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 11:46:33,083] [INFO] DFAST Taxonomy check result was written to GCA_018699085.1_ASM1869908v1_genomic.fna/tc_result.tsv
[2023-06-19 11:46:33,084] [INFO] ===== Taxonomy check completed =====
[2023-06-19 11:46:33,084] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 11:46:33,084] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/checkm_data
[2023-06-19 11:46:33,087] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 11:46:33,122] [INFO] Task started: CheckM
[2023-06-19 11:46:33,122] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018699085.1_ASM1869908v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018699085.1_ASM1869908v1_genomic.fna/checkm_input GCA_018699085.1_ASM1869908v1_genomic.fna/checkm_result
[2023-06-19 11:47:01,728] [INFO] Task succeeded: CheckM
[2023-06-19 11:47:01,728] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 11:47:01,742] [INFO] ===== Completeness check finished =====
[2023-06-19 11:47:01,743] [INFO] ===== Start GTDB Search =====
[2023-06-19 11:47:01,743] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018699085.1_ASM1869908v1_genomic.fna/markers.fasta)
[2023-06-19 11:47:01,743] [INFO] Task started: Blastn
[2023-06-19 11:47:01,743] [INFO] Running command: blastn -query GCA_018699085.1_ASM1869908v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf419c529-ffb5-44d1-aa1f-b0e38f612a7b/dqc_reference/reference_markers_gtdb.fasta -out GCA_018699085.1_ASM1869908v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 11:47:02,818] [INFO] Task succeeded: Blastn
[2023-06-19 11:47:02,821] [INFO] Selected 23 target genomes.
[2023-06-19 11:47:02,821] [INFO] Target genome list was writen to GCA_018699085.1_ASM1869908v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 11:47:02,829] [INFO] Task started: fastANI
[2023-06-19 11:47:02,829] [INFO] Running command: fastANI --query /var/lib/cwl/stg41cb9e8b-ce49-4314-b6c0-edba9b98fdaf/GCA_018699085.1_ASM1869908v1_genomic.fna.gz --refList GCA_018699085.1_ASM1869908v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018699085.1_ASM1869908v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 11:47:16,704] [INFO] Task succeeded: fastANI
[2023-06-19 11:47:16,707] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 11:47:16,707] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018666155.1	s__WLXB01 sp018666155	99.9445	1089	1124	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodospirillales;f__Rhodospirillaceae;g__WLXB01	95.0	99.93	99.91	0.96	0.93	5	conclusive
GCA_012103295.1	s__WLXB01 sp012103295	80.28	728	1124	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodospirillales;f__Rhodospirillaceae;g__WLXB01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-19 11:47:16,708] [INFO] GTDB search result was written to GCA_018699085.1_ASM1869908v1_genomic.fna/result_gtdb.tsv
[2023-06-19 11:47:16,709] [INFO] ===== GTDB Search completed =====
[2023-06-19 11:47:16,711] [INFO] DFAST_QC result json was written to GCA_018699085.1_ASM1869908v1_genomic.fna/dqc_result.json
[2023-06-19 11:47:16,711] [INFO] DFAST_QC completed!
[2023-06-19 11:47:16,711] [INFO] Total running time: 0h1m18s