[2023-06-18 19:41:57,468] [INFO] DFAST_QC pipeline started.
[2023-06-18 19:41:57,473] [INFO] DFAST_QC version: 0.5.7
[2023-06-18 19:41:57,473] [INFO] DQC Reference Directory: /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference
[2023-06-18 19:41:59,501] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-18 19:41:59,502] [INFO] Task started: Prodigal
[2023-06-18 19:41:59,503] [INFO] Running command: gunzip -c /var/lib/cwl/stgcff49c77-32ff-4ccd-9360-e2f09507e1bb/GCA_018820975.1_ASM1882097v1_genomic.fna.gz | prodigal -d GCA_018820975.1_ASM1882097v1_genomic.fna/cds.fna -a GCA_018820975.1_ASM1882097v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-18 19:42:01,550] [INFO] Task succeeded: Prodigal
[2023-06-18 19:42:01,551] [INFO] Task started: HMMsearch
[2023-06-18 19:42:01,551] [INFO] Running command: hmmsearch --tblout GCA_018820975.1_ASM1882097v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/reference_markers.hmm GCA_018820975.1_ASM1882097v1_genomic.fna/protein.faa > /dev/null
[2023-06-18 19:42:01,737] [INFO] Task succeeded: HMMsearch
[2023-06-18 19:42:01,739] [INFO] Found 6/6 markers.
[2023-06-18 19:42:01,758] [INFO] Query marker FASTA was written to GCA_018820975.1_ASM1882097v1_genomic.fna/markers.fasta
[2023-06-18 19:42:01,759] [INFO] Task started: Blastn
[2023-06-18 19:42:01,759] [INFO] Running command: blastn -query GCA_018820975.1_ASM1882097v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/reference_markers.fasta -out GCA_018820975.1_ASM1882097v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 19:42:03,942] [INFO] Task succeeded: Blastn
[2023-06-18 19:42:03,946] [INFO] Selected 14 target genomes.
[2023-06-18 19:42:03,947] [INFO] Target genome list was writen to GCA_018820975.1_ASM1882097v1_genomic.fna/target_genomes.txt
[2023-06-18 19:42:03,956] [INFO] Task started: fastANI
[2023-06-18 19:42:03,956] [INFO] Running command: fastANI --query /var/lib/cwl/stgcff49c77-32ff-4ccd-9360-e2f09507e1bb/GCA_018820975.1_ASM1882097v1_genomic.fna.gz --refList GCA_018820975.1_ASM1882097v1_genomic.fna/target_genomes.txt --output GCA_018820975.1_ASM1882097v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-18 19:42:10,641] [INFO] Task succeeded: fastANI
[2023-06-18 19:42:10,642] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-18 19:42:10,642] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-18 19:42:10,644] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-18 19:42:10,645] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-18 19:42:10,645] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-18 19:42:10,647] [INFO] DFAST Taxonomy check result was written to GCA_018820975.1_ASM1882097v1_genomic.fna/tc_result.tsv
[2023-06-18 19:42:10,648] [INFO] ===== Taxonomy check completed =====
[2023-06-18 19:42:10,648] [INFO] ===== Start completeness check using CheckM =====
[2023-06-18 19:42:10,648] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/checkm_data
[2023-06-18 19:42:10,652] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-18 19:42:10,664] [INFO] Task started: CheckM
[2023-06-18 19:42:10,664] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018820975.1_ASM1882097v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018820975.1_ASM1882097v1_genomic.fna/checkm_input GCA_018820975.1_ASM1882097v1_genomic.fna/checkm_result
[2023-06-18 19:42:24,877] [INFO] Task succeeded: CheckM
[2023-06-18 19:42:24,879] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 77.78%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-18 19:42:24,899] [INFO] ===== Completeness check finished =====
[2023-06-18 19:42:24,899] [INFO] ===== Start GTDB Search =====
[2023-06-18 19:42:24,900] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018820975.1_ASM1882097v1_genomic.fna/markers.fasta)
[2023-06-18 19:42:24,900] [INFO] Task started: Blastn
[2023-06-18 19:42:24,900] [INFO] Running command: blastn -query GCA_018820975.1_ASM1882097v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeb22ab1a-0660-4620-ae61-d31bdc7e5286/dqc_reference/reference_markers_gtdb.fasta -out GCA_018820975.1_ASM1882097v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 19:42:25,636] [INFO] Task succeeded: Blastn
[2023-06-18 19:42:25,641] [INFO] Selected 19 target genomes.
[2023-06-18 19:42:25,641] [INFO] Target genome list was writen to GCA_018820975.1_ASM1882097v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-18 19:42:25,651] [INFO] Task started: fastANI
[2023-06-18 19:42:25,651] [INFO] Running command: fastANI --query /var/lib/cwl/stgcff49c77-32ff-4ccd-9360-e2f09507e1bb/GCA_018820975.1_ASM1882097v1_genomic.fna.gz --refList GCA_018820975.1_ASM1882097v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018820975.1_ASM1882097v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-18 19:42:28,815] [INFO] Task succeeded: fastANI
[2023-06-18 19:42:28,819] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-18 19:42:28,820] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018822835.1	s__JAHJJO01 sp018822835	97.3527	112	146	d__Bacteria;p__Patescibacteria;c__ABY1;o__BM507;f__JAHJJO01;g__JAHJJO01	95.0	98.52	97.60	0.77	0.75	5	conclusive
--------------------------------------------------------------------------------
[2023-06-18 19:42:28,821] [INFO] GTDB search result was written to GCA_018820975.1_ASM1882097v1_genomic.fna/result_gtdb.tsv
[2023-06-18 19:42:28,822] [INFO] ===== GTDB Search completed =====
[2023-06-18 19:42:28,824] [INFO] DFAST_QC result json was written to GCA_018820975.1_ASM1882097v1_genomic.fna/dqc_result.json
[2023-06-18 19:42:28,825] [INFO] DFAST_QC completed!
[2023-06-18 19:42:28,825] [INFO] Total running time: 0h0m31s