[2023-06-19 08:34:24,027] [INFO] DFAST_QC pipeline started.
[2023-06-19 08:34:24,031] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 08:34:24,031] [INFO] DQC Reference Directory: /var/lib/cwl/stgae66b55f-002b-4408-9f26-4315dfb83e16/dqc_reference
[2023-06-19 08:34:25,271] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 08:34:25,272] [INFO] Task started: Prodigal
[2023-06-19 08:34:25,272] [INFO] Running command: gunzip -c /var/lib/cwl/stga2d7bbc6-d1b8-4073-a9f3-5dbd81b829d9/GCA_018897955.1_ASM1889795v1_genomic.fna.gz | prodigal -d GCA_018897955.1_ASM1889795v1_genomic.fna/cds.fna -a GCA_018897955.1_ASM1889795v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 08:34:27,366] [INFO] Task succeeded: Prodigal
[2023-06-19 08:34:27,367] [INFO] Task started: HMMsearch
[2023-06-19 08:34:27,367] [INFO] Running command: hmmsearch --tblout GCA_018897955.1_ASM1889795v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgae66b55f-002b-4408-9f26-4315dfb83e16/dqc_reference/reference_markers.hmm GCA_018897955.1_ASM1889795v1_genomic.fna/protein.faa > /dev/null
[2023-06-19 08:34:27,569] [INFO] Task succeeded: HMMsearch
[2023-06-19 08:34:27,571] [WARNING] Found 2/6 markers. [/var/lib/cwl/stga2d7bbc6-d1b8-4073-a9f3-5dbd81b829d9/GCA_018897955.1_ASM1889795v1_genomic.fna.gz]
[2023-06-19 08:34:27,589] [INFO] Query marker FASTA was written to GCA_018897955.1_ASM1889795v1_genomic.fna/markers.fasta
[2023-06-19 08:34:27,589] [INFO] Task started: Blastn
[2023-06-19 08:34:27,590] [INFO] Running command: blastn -query GCA_018897955.1_ASM1889795v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgae66b55f-002b-4408-9f26-4315dfb83e16/dqc_reference/reference_markers.fasta -out GCA_018897955.1_ASM1889795v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 08:34:28,154] [INFO] Task succeeded: Blastn
[2023-06-19 08:34:28,157] [INFO] Selected 0 target genomes.
[2023-06-19 08:34:28,158] [INFO] Target genome list was writen to GCA_018897955.1_ASM1889795v1_genomic.fna/target_genomes.txt
[2023-06-19 08:34:28,158] [ERROR] File is empty. [GCA_018897955.1_ASM1889795v1_genomic.fna/target_genomes.txt]
[2023-06-19 08:34:28,158] [ERROR] Task failed. No target genome found.
[2023-06-19 08:34:28,158] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 08:34:28,158] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgae66b55f-002b-4408-9f26-4315dfb83e16/dqc_reference/checkm_data
[2023-06-19 08:34:28,161] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 08:34:28,175] [INFO] Task started: CheckM
[2023-06-19 08:34:28,175] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_018897955.1_ASM1889795v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_018897955.1_ASM1889795v1_genomic.fna/checkm_input GCA_018897955.1_ASM1889795v1_genomic.fna/checkm_result
[2023-06-19 08:34:42,819] [INFO] Task succeeded: CheckM
[2023-06-19 08:34:42,820] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 78.39%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 08:34:42,844] [INFO] ===== Completeness check finished =====
[2023-06-19 08:34:42,844] [INFO] ===== Start GTDB Search =====
[2023-06-19 08:34:42,845] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_018897955.1_ASM1889795v1_genomic.fna/markers.fasta)
[2023-06-19 08:34:42,845] [INFO] Task started: Blastn
[2023-06-19 08:34:42,845] [INFO] Running command: blastn -query GCA_018897955.1_ASM1889795v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgae66b55f-002b-4408-9f26-4315dfb83e16/dqc_reference/reference_markers_gtdb.fasta -out GCA_018897955.1_ASM1889795v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 08:34:43,522] [INFO] Task succeeded: Blastn
[2023-06-19 08:34:43,529] [INFO] Selected 1 target genomes.
[2023-06-19 08:34:43,529] [INFO] Target genome list was writen to GCA_018897955.1_ASM1889795v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 08:34:43,532] [INFO] Task started: fastANI
[2023-06-19 08:34:43,532] [INFO] Running command: fastANI --query /var/lib/cwl/stga2d7bbc6-d1b8-4073-a9f3-5dbd81b829d9/GCA_018897955.1_ASM1889795v1_genomic.fna.gz --refList GCA_018897955.1_ASM1889795v1_genomic.fna/target_genomes_gtdb.txt --output GCA_018897955.1_ASM1889795v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 08:34:43,937] [INFO] Task succeeded: fastANI
[2023-06-19 08:34:43,942] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-19 08:34:43,942] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018897155.1	s__LFW-46 sp018897155	99.8106	235	239	d__Archaea;p__EX4484-52;c__EX4484-52;o__EX4484-52;f__LFW-46;g__LFW-46	95.0	99.88	99.81	0.97	0.96	3	conclusive
--------------------------------------------------------------------------------
[2023-06-19 08:34:43,946] [INFO] GTDB search result was written to GCA_018897955.1_ASM1889795v1_genomic.fna/result_gtdb.tsv
[2023-06-19 08:34:43,947] [INFO] ===== GTDB Search completed =====
[2023-06-19 08:34:43,950] [INFO] DFAST_QC result json was written to GCA_018897955.1_ASM1889795v1_genomic.fna/dqc_result.json
[2023-06-19 08:34:43,950] [INFO] DFAST_QC completed!
[2023-06-19 08:34:43,950] [INFO] Total running time: 0h0m20s