[2023-06-28 19:21:39,719] [INFO] DFAST_QC pipeline started.
[2023-06-28 19:21:39,722] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 19:21:39,722] [INFO] DQC Reference Directory: /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference
[2023-06-28 19:21:41,289] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 19:21:41,290] [INFO] Task started: Prodigal
[2023-06-28 19:21:41,290] [INFO] Running command: gunzip -c /var/lib/cwl/stgd64e569b-4b73-478b-ad63-ad59d29b5af5/GCA_019424845.1_ASM1942484v1_genomic.fna.gz | prodigal -d GCA_019424845.1_ASM1942484v1_genomic.fna/cds.fna -a GCA_019424845.1_ASM1942484v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 19:21:47,690] [INFO] Task succeeded: Prodigal
[2023-06-28 19:21:47,691] [INFO] Task started: HMMsearch
[2023-06-28 19:21:47,691] [INFO] Running command: hmmsearch --tblout GCA_019424845.1_ASM1942484v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/reference_markers.hmm GCA_019424845.1_ASM1942484v1_genomic.fna/protein.faa > /dev/null
[2023-06-28 19:21:47,919] [INFO] Task succeeded: HMMsearch
[2023-06-28 19:21:47,920] [INFO] Found 6/6 markers.
[2023-06-28 19:21:47,941] [INFO] Query marker FASTA was written to GCA_019424845.1_ASM1942484v1_genomic.fna/markers.fasta
[2023-06-28 19:21:47,941] [INFO] Task started: Blastn
[2023-06-28 19:21:47,942] [INFO] Running command: blastn -query GCA_019424845.1_ASM1942484v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/reference_markers.fasta -out GCA_019424845.1_ASM1942484v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 19:21:48,932] [INFO] Task succeeded: Blastn
[2023-06-28 19:21:48,936] [INFO] Selected 10 target genomes.
[2023-06-28 19:21:48,936] [INFO] Target genome list was writen to GCA_019424845.1_ASM1942484v1_genomic.fna/target_genomes.txt
[2023-06-28 19:21:48,939] [INFO] Task started: fastANI
[2023-06-28 19:21:48,939] [INFO] Running command: fastANI --query /var/lib/cwl/stgd64e569b-4b73-478b-ad63-ad59d29b5af5/GCA_019424845.1_ASM1942484v1_genomic.fna.gz --refList GCA_019424845.1_ASM1942484v1_genomic.fna/target_genomes.txt --output GCA_019424845.1_ASM1942484v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 19:21:53,863] [INFO] Task succeeded: fastANI
[2023-06-28 19:21:53,864] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 19:21:53,864] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 19:21:53,884] [INFO] Found 7 fastANI hits (5 hits with ANI > threshold)
[2023-06-28 19:21:53,884] [INFO] The taxonomy check result is classified as 'conclusive'.
[2023-06-28 19:21:53,885] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bifidobacterium longum	strain=DSM 20219	GCA_900104835.1	216816	216816	type	True	96.6741	459	582	95	conclusive
Bifidobacterium longum subsp. longum	strain=KCTC 3128	GCA_017132775.1	1679	216816	type	True	96.6704	453	582	95	conclusive
Bifidobacterium longum subsp. longum	strain=LMG 13197	GCA_000741245.1	1679	216816	type	True	96.4994	462	582	95	conclusive
Bifidobacterium longum subsp. longum	strain=JCM 1217	GCA_000196555.1	1679	216816	type	True	96.4977	467	582	95	conclusive
Bifidobacterium longum	strain=NCTC11818	GCA_900637335.1	216816	216816	type	True	96.4972	467	582	95	conclusive
Bifidobacterium miconisargentati	strain=82T25	GCA_019331675.1	2834437	2834437	type	True	83.73	396	582	95	below_threshold
Bifidobacterium mizhiense	strain=S053-2	GCA_020884755.1	2879940	2879940	type	True	76.8298	105	582	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-28 19:21:53,891] [INFO] DFAST Taxonomy check result was written to GCA_019424845.1_ASM1942484v1_genomic.fna/tc_result.tsv
[2023-06-28 19:21:53,891] [INFO] ===== Taxonomy check completed =====
[2023-06-28 19:21:53,892] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 19:21:53,892] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/checkm_data
[2023-06-28 19:21:53,894] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 19:21:53,940] [INFO] Task started: CheckM
[2023-06-28 19:21:53,941] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_019424845.1_ASM1942484v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_019424845.1_ASM1942484v1_genomic.fna/checkm_input GCA_019424845.1_ASM1942484v1_genomic.fna/checkm_result
[2023-06-28 19:22:19,145] [INFO] Task succeeded: CheckM
[2023-06-28 19:22:19,146] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 80.40%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-28 19:22:19,166] [INFO] ===== Completeness check finished =====
[2023-06-28 19:22:19,166] [INFO] ===== Start GTDB Search =====
[2023-06-28 19:22:19,167] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_019424845.1_ASM1942484v1_genomic.fna/markers.fasta)
[2023-06-28 19:22:19,167] [INFO] Task started: Blastn
[2023-06-28 19:22:19,167] [INFO] Running command: blastn -query GCA_019424845.1_ASM1942484v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7dfbdc6d-072c-4353-b039-6f9dbaabaacf/dqc_reference/reference_markers_gtdb.fasta -out GCA_019424845.1_ASM1942484v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 19:22:20,809] [INFO] Task succeeded: Blastn
[2023-06-28 19:22:20,813] [INFO] Selected 9 target genomes.
[2023-06-28 19:22:20,814] [INFO] Target genome list was writen to GCA_019424845.1_ASM1942484v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 19:22:20,816] [INFO] Task started: fastANI
[2023-06-28 19:22:20,816] [INFO] Running command: fastANI --query /var/lib/cwl/stgd64e569b-4b73-478b-ad63-ad59d29b5af5/GCA_019424845.1_ASM1942484v1_genomic.fna.gz --refList GCA_019424845.1_ASM1942484v1_genomic.fna/target_genomes_gtdb.txt --output GCA_019424845.1_ASM1942484v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 19:22:25,593] [INFO] Task succeeded: fastANI
[2023-06-28 19:22:25,601] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-28 19:22:25,601] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000196555.1	s__Bifidobacterium longum	96.4977	467	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.53	95.59	0.86	0.67	481	conclusive
GCF_000269965.1	s__Bifidobacterium infantis	94.6123	438	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.68	97.44	0.91	0.82	49	-
GCF_001025175.1	s__Bifidobacterium breve	86.136	395	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.58	97.75	0.90	0.86	170	-
GCF_018555355.1	s__Bifidobacterium sp018555355	83.8556	400	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.26	96.26	0.85	0.85	2	-
GCF_003129905.1	s__Bifidobacterium callitrichidarum	83.3854	418	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002259745.1	s__Bifidobacterium myosotis	82.8934	392	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.86	96.74	0.90	0.89	3	-
GCF_012932425.1	s__Bifidobacterium sp012932425	81.0232	293	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000741165.1	s__Bifidobacterium biavatii	80.8282	289	582	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.97	99.97	0.98	0.98	2	-
--------------------------------------------------------------------------------
[2023-06-28 19:22:25,603] [INFO] GTDB search result was written to GCA_019424845.1_ASM1942484v1_genomic.fna/result_gtdb.tsv
[2023-06-28 19:22:25,604] [INFO] ===== GTDB Search completed =====
[2023-06-28 19:22:25,608] [INFO] DFAST_QC result json was written to GCA_019424845.1_ASM1942484v1_genomic.fna/dqc_result.json
[2023-06-28 19:22:25,608] [INFO] DFAST_QC completed!
[2023-06-28 19:22:25,608] [INFO] Total running time: 0h0m46s