[2023-06-28 11:04:04,668] [INFO] DFAST_QC pipeline started.
[2023-06-28 11:04:04,671] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 11:04:04,672] [INFO] DQC Reference Directory: /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference
[2023-06-28 11:04:06,139] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 11:04:06,140] [INFO] Task started: Prodigal
[2023-06-28 11:04:06,140] [INFO] Running command: gunzip -c /var/lib/cwl/stg3523521b-7eb3-4057-810f-8f6a572afa68/GCA_020052475.1_ASM2005247v1_genomic.fna.gz | prodigal -d GCA_020052475.1_ASM2005247v1_genomic.fna/cds.fna -a GCA_020052475.1_ASM2005247v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 11:04:08,523] [INFO] Task succeeded: Prodigal
[2023-06-28 11:04:08,524] [INFO] Task started: HMMsearch
[2023-06-28 11:04:08,524] [INFO] Running command: hmmsearch --tblout GCA_020052475.1_ASM2005247v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/reference_markers.hmm GCA_020052475.1_ASM2005247v1_genomic.fna/protein.faa > /dev/null
[2023-06-28 11:04:08,715] [INFO] Task succeeded: HMMsearch
[2023-06-28 11:04:08,719] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg3523521b-7eb3-4057-810f-8f6a572afa68/GCA_020052475.1_ASM2005247v1_genomic.fna.gz]
[2023-06-28 11:04:08,739] [INFO] Query marker FASTA was written to GCA_020052475.1_ASM2005247v1_genomic.fna/markers.fasta
[2023-06-28 11:04:08,739] [INFO] Task started: Blastn
[2023-06-28 11:04:08,740] [INFO] Running command: blastn -query GCA_020052475.1_ASM2005247v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/reference_markers.fasta -out GCA_020052475.1_ASM2005247v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 11:04:10,126] [INFO] Task succeeded: Blastn
[2023-06-28 11:04:10,131] [INFO] Selected 13 target genomes.
[2023-06-28 11:04:10,132] [INFO] Target genome list was writen to GCA_020052475.1_ASM2005247v1_genomic.fna/target_genomes.txt
[2023-06-28 11:04:10,139] [INFO] Task started: fastANI
[2023-06-28 11:04:10,140] [INFO] Running command: fastANI --query /var/lib/cwl/stg3523521b-7eb3-4057-810f-8f6a572afa68/GCA_020052475.1_ASM2005247v1_genomic.fna.gz --refList GCA_020052475.1_ASM2005247v1_genomic.fna/target_genomes.txt --output GCA_020052475.1_ASM2005247v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 11:04:17,765] [INFO] Task succeeded: fastANI
[2023-06-28 11:04:17,766] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 11:04:17,767] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 11:04:17,769] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-28 11:04:17,769] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-28 11:04:17,769] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-28 11:04:17,771] [INFO] DFAST Taxonomy check result was written to GCA_020052475.1_ASM2005247v1_genomic.fna/tc_result.tsv
[2023-06-28 11:04:17,772] [INFO] ===== Taxonomy check completed =====
[2023-06-28 11:04:17,773] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 11:04:17,773] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/checkm_data
[2023-06-28 11:04:17,777] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 11:04:17,796] [INFO] Task started: CheckM
[2023-06-28 11:04:17,796] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_020052475.1_ASM2005247v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_020052475.1_ASM2005247v1_genomic.fna/checkm_input GCA_020052475.1_ASM2005247v1_genomic.fna/checkm_result
[2023-06-28 11:04:33,200] [INFO] Task succeeded: CheckM
[2023-06-28 11:04:33,202] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 66.67%
Contamintation: 0.76%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-06-28 11:04:33,223] [INFO] ===== Completeness check finished =====
[2023-06-28 11:04:33,223] [INFO] ===== Start GTDB Search =====
[2023-06-28 11:04:33,224] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_020052475.1_ASM2005247v1_genomic.fna/markers.fasta)
[2023-06-28 11:04:33,224] [INFO] Task started: Blastn
[2023-06-28 11:04:33,225] [INFO] Running command: blastn -query GCA_020052475.1_ASM2005247v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3036a7ab-86ba-485f-9d11-a235967d67b1/dqc_reference/reference_markers_gtdb.fasta -out GCA_020052475.1_ASM2005247v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 11:04:33,970] [INFO] Task succeeded: Blastn
[2023-06-28 11:04:33,974] [INFO] Selected 17 target genomes.
[2023-06-28 11:04:33,975] [INFO] Target genome list was writen to GCA_020052475.1_ASM2005247v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 11:04:34,007] [INFO] Task started: fastANI
[2023-06-28 11:04:34,008] [INFO] Running command: fastANI --query /var/lib/cwl/stg3523521b-7eb3-4057-810f-8f6a572afa68/GCA_020052475.1_ASM2005247v1_genomic.fna.gz --refList GCA_020052475.1_ASM2005247v1_genomic.fna/target_genomes_gtdb.txt --output GCA_020052475.1_ASM2005247v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 11:04:40,820] [INFO] Task succeeded: fastANI
[2023-06-28 11:04:40,822] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-28 11:04:40,822] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-28 11:04:40,825] [INFO] GTDB search result was written to GCA_020052475.1_ASM2005247v1_genomic.fna/result_gtdb.tsv
[2023-06-28 11:04:40,826] [INFO] ===== GTDB Search completed =====
[2023-06-28 11:04:40,828] [INFO] DFAST_QC result json was written to GCA_020052475.1_ASM2005247v1_genomic.fna/dqc_result.json
[2023-06-28 11:04:40,829] [INFO] DFAST_QC completed!
[2023-06-28 11:04:40,829] [INFO] Total running time: 0h0m36s