[2023-06-28 12:00:02,773] [INFO] DFAST_QC pipeline started.
[2023-06-28 12:00:02,775] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 12:00:02,776] [INFO] DQC Reference Directory: /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference
[2023-06-28 12:00:04,152] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 12:00:04,153] [INFO] Task started: Prodigal
[2023-06-28 12:00:04,154] [INFO] Running command: gunzip -c /var/lib/cwl/stg1018076e-52e1-4ec5-bcae-054c11d0bcff/GCA_020635975.1_ASM2063597v1_genomic.fna.gz | prodigal -d GCA_020635975.1_ASM2063597v1_genomic.fna/cds.fna -a GCA_020635975.1_ASM2063597v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 12:00:13,264] [INFO] Task succeeded: Prodigal
[2023-06-28 12:00:13,264] [INFO] Task started: HMMsearch
[2023-06-28 12:00:13,264] [INFO] Running command: hmmsearch --tblout GCA_020635975.1_ASM2063597v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/reference_markers.hmm GCA_020635975.1_ASM2063597v1_genomic.fna/protein.faa > /dev/null
[2023-06-28 12:00:13,506] [INFO] Task succeeded: HMMsearch
[2023-06-28 12:00:13,508] [INFO] Found 6/6 markers.
[2023-06-28 12:00:13,543] [INFO] Query marker FASTA was written to GCA_020635975.1_ASM2063597v1_genomic.fna/markers.fasta
[2023-06-28 12:00:13,544] [INFO] Task started: Blastn
[2023-06-28 12:00:13,544] [INFO] Running command: blastn -query GCA_020635975.1_ASM2063597v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/reference_markers.fasta -out GCA_020635975.1_ASM2063597v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 12:00:14,142] [INFO] Task succeeded: Blastn
[2023-06-28 12:00:14,147] [INFO] Selected 31 target genomes.
[2023-06-28 12:00:14,148] [INFO] Target genome list was writen to GCA_020635975.1_ASM2063597v1_genomic.fna/target_genomes.txt
[2023-06-28 12:00:14,158] [INFO] Task started: fastANI
[2023-06-28 12:00:14,158] [INFO] Running command: fastANI --query /var/lib/cwl/stg1018076e-52e1-4ec5-bcae-054c11d0bcff/GCA_020635975.1_ASM2063597v1_genomic.fna.gz --refList GCA_020635975.1_ASM2063597v1_genomic.fna/target_genomes.txt --output GCA_020635975.1_ASM2063597v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 12:00:31,737] [INFO] Task succeeded: fastANI
[2023-06-28 12:00:31,737] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 12:00:31,737] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 12:00:31,739] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-28 12:00:31,739] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-28 12:00:31,739] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-28 12:00:31,741] [INFO] DFAST Taxonomy check result was written to GCA_020635975.1_ASM2063597v1_genomic.fna/tc_result.tsv
[2023-06-28 12:00:31,742] [INFO] ===== Taxonomy check completed =====
[2023-06-28 12:00:31,742] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 12:00:31,742] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/checkm_data
[2023-06-28 12:00:31,745] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 12:00:31,781] [INFO] Task started: CheckM
[2023-06-28 12:00:31,782] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_020635975.1_ASM2063597v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_020635975.1_ASM2063597v1_genomic.fna/checkm_input GCA_020635975.1_ASM2063597v1_genomic.fna/checkm_result
[2023-06-28 12:01:02,943] [INFO] Task succeeded: CheckM
[2023-06-28 12:01:02,945] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-28 12:01:02,965] [INFO] ===== Completeness check finished =====
[2023-06-28 12:01:02,966] [INFO] ===== Start GTDB Search =====
[2023-06-28 12:01:02,966] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_020635975.1_ASM2063597v1_genomic.fna/markers.fasta)
[2023-06-28 12:01:02,967] [INFO] Task started: Blastn
[2023-06-28 12:01:02,967] [INFO] Running command: blastn -query GCA_020635975.1_ASM2063597v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgaaf83e2c-41c0-4016-a8e8-88e8a882fcc5/dqc_reference/reference_markers_gtdb.fasta -out GCA_020635975.1_ASM2063597v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 12:01:03,711] [INFO] Task succeeded: Blastn
[2023-06-28 12:01:03,716] [INFO] Selected 34 target genomes.
[2023-06-28 12:01:03,717] [INFO] Target genome list was writen to GCA_020635975.1_ASM2063597v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 12:01:03,782] [INFO] Task started: fastANI
[2023-06-28 12:01:03,783] [INFO] Running command: fastANI --query /var/lib/cwl/stg1018076e-52e1-4ec5-bcae-054c11d0bcff/GCA_020635975.1_ASM2063597v1_genomic.fna.gz --refList GCA_020635975.1_ASM2063597v1_genomic.fna/target_genomes_gtdb.txt --output GCA_020635975.1_ASM2063597v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 12:01:22,621] [INFO] Task succeeded: fastANI
[2023-06-28 12:01:22,624] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-28 12:01:22,625] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_017853115.1	s__JAFMKE01 sp017853115	75.8414	62	857	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Chitinophagales;f__UBA8649;g__JAFMKE01	95.0	99.30	99.30	0.86	0.86	2	-
--------------------------------------------------------------------------------
[2023-06-28 12:01:22,627] [INFO] GTDB search result was written to GCA_020635975.1_ASM2063597v1_genomic.fna/result_gtdb.tsv
[2023-06-28 12:01:22,627] [INFO] ===== GTDB Search completed =====
[2023-06-28 12:01:22,630] [INFO] DFAST_QC result json was written to GCA_020635975.1_ASM2063597v1_genomic.fna/dqc_result.json
[2023-06-28 12:01:22,630] [INFO] DFAST_QC completed!
[2023-06-28 12:01:22,630] [INFO] Total running time: 0h1m20s