<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2021-11-02T18:40:26.907" last_update="2021-11-02T18:40:26.907" submission_date="2021-08-23T08:11:08.970" id="20928774" accession="SAMN20928774">   <Ids>     <Id db="BioSample" is_primary="1">SAMN20928774</Id>     <Id db_label="Sample name">GA_180221_E-1-1_metaspades_Cyanobacteria_43_4</Id>   </Ids>   <Description>     <Title>MIMAG Metagenome-assembled Genome sample from Microcoleus sp. GA_180221_E-1-1-MAG-43_4</Title>     <Organism taxonomy_id="2872243" taxonomy_name="Microcoleus sp. GA_180221_E-1-1-MAG-43_4">       <OrganismName>Microcoleus sp. GA_180221_E-1-1-MAG-43_4</OrganismName>     </Organism>     <Comment>       <Paragraph>groundwater sample from cold water geyser eruptions; erupting water was collected in bleach-sterilized containers and from there filtered on teflon filters with 0.1 um pore size</Paragraph>       <Paragraph>Keywords: GSC:MIxS;MIMAG:6.0</Paragraph>     </Comment>   </Description>   <Owner>     <Name>University Duisburg-Essen</Name>     <Contacts>       <Contact email="till.bornemann@uni-due.de">         <Name>           <First>Till</First>           <Last>Bornemann</Last>           <Middle>LV</Middle>         </Name>       </Contact>     </Contacts>   </Owner>   <Models>     <Model>MIMAG.water</Model>   </Models>   <Package display_name="MIMAG: metagenome-assembled genome, water; version 6.0">MIMAG.water.6.0</Package>   <Attributes>     <Attribute attribute_name="depth" harmonized_name="depth" display_name="depth">351 m</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">groundwater from cold water geyser, filtered on 0.1um pore size filters</Attribute>     <Attribute attribute_name="collection_date" harmonized_name="collection_date" display_name="collection date">2018-02-21</Attribute>     <Attribute attribute_name="geo_loc_name" harmonized_name="geo_loc_name" display_name="geographic location">Germany: Volcanic Eifel, Andernach</Attribute>     <Attribute attribute_name="lat_lon" harmonized_name="lat_lon" display_name="latitude and longitude">50.448588 N 7.375355 E</Attribute>     <Attribute attribute_name="isolate-name-alias" harmonized_name="isolate_name_alias" display_name="isolate name alias">GA_180221_E-1-1_metaspades_Cyanobacteria_43_4</Attribute>     <Attribute attribute_name="env_broad_scale" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">groundwater|terrestrial subsurface|cold water geyser</Attribute>     <Attribute attribute_name="env_local_scale" harmonized_name="env_local_scale" display_name="local-scale environmental context">cold water geyser|geyser eruption</Attribute>     <Attribute attribute_name="env_medium" harmonized_name="env_medium" display_name="environmental medium">groundwater</Attribute>     <Attribute attribute_name="diss_org_carb" harmonized_name="diss_org_carb" display_name="dissolved organic carbon">1.39 (0.31) mg/L</Attribute>     <Attribute attribute_name="potassium" harmonized_name="potassium" display_name="potassium">3.15 (0.86) mM</Attribute>     <Attribute attribute_name="sulfate" harmonized_name="sulfate" display_name="sulfate">4.98 (0.39) mM</Attribute>     <Attribute attribute_name="calcium" harmonized_name="calcium" display_name="calcium">5.26 (0.21) mM</Attribute>     <Attribute attribute_name="chloride" harmonized_name="chloride" display_name="chloride">70.58 (7.26) mM</Attribute>     <Attribute attribute_name="sodium" harmonized_name="sodium" display_name="sodium">73.05 (2.28) mM</Attribute>     <Attribute attribute_name="magnesium" harmonized_name="magnesium" display_name="magnesium">9.05 (0.27)</Attribute>     <Attribute attribute_name="nitrate" harmonized_name="nitrate" display_name="nitrate">BDL</Attribute>     <Attribute attribute_name="phosphate" harmonized_name="phosphate" display_name="phosphate">BDL</Attribute>     <Attribute attribute_name="sulfide" harmonized_name="sulfide" display_name="sulfide">BDL</Attribute>     <Attribute attribute_name="chem_administration" harmonized_name="chem_administration" display_name="chemical administration">None</Attribute>     <Attribute attribute_name="conduc" harmonized_name="conduc" display_name="conductivity">12800-13600 S</Attribute>     <Attribute attribute_name="diss_carb_dioxide" harmonized_name="diss_carb_dioxide" display_name="dissolved carbon dioxide">5700 mg/L</Attribute>     <Attribute attribute_name="organism_count" harmonized_name="organism_count" display_name="organism count">3.5e10^6cells/mL total cells; eFM using DAPI</Attribute>     <Attribute attribute_name="oxy_stat_samp" harmonized_name="oxy_stat_samp" display_name="oxygenation status of sample">anaerobic</Attribute>     <Attribute attribute_name="ph" harmonized_name="ph" display_name="pH">6.8</Attribute>     <Attribute attribute_name="ref_biomaterial" harmonized_name="ref_biomaterial" display_name="reference for biomaterial">https://www.biorxiv.org/content/10.1101/2020.03.07.980714v3.full.pdf</Attribute>     <Attribute attribute_name="samp_collect_device" harmonized_name="samp_collect_device" display_name="sample collection device or method">0.1 um pore size teflon filters</Attribute>     <Attribute attribute_name="samp_mat_process" harmonized_name="samp_mat_process" display_name="sample material processing">DNA Powersoil max kit followed by EtOH precipitation with Glycogen carrier</Attribute>     <Attribute attribute_name="samp_size" harmonized_name="samp_size" display_name="sample size">30 L of filtered volume; 1/2 of filter was used for extraction</Attribute>     <Attribute attribute_name="samp_store_dur" harmonized_name="samp_store_dur" display_name="sample storage duration">&lt; 1 week</Attribute>     <Attribute attribute_name="samp_store_loc" harmonized_name="samp_store_loc" display_name="sample storage location">- on dry ice (field) or in 80 C fridge (lab)</Attribute>     <Attribute attribute_name="samp_store_temp" harmonized_name="samp_store_temp" display_name="sample storage temperature">-80 C</Attribute>     <Attribute attribute_name="samp_vol_we_dna_ext" harmonized_name="samp_vol_we_dna_ext" display_name="sample volume or weight for DNA extraction">~ 15 L (1/2 of a filter)</Attribute>     <Attribute attribute_name="size_frac" harmonized_name="size_frac" display_name="size fraction selected">0.1 m</Attribute>     <Attribute attribute_name="temp" harmonized_name="temp" display_name="temperature">18 C</Attribute>     <Attribute attribute_name="tot_depth_water_col" harmonized_name="tot_depth_water_col" display_name="total depth of water column">351 m</Attribute>     <Attribute attribute_name="total_iron" harmonized_name="tot_iron" display_name="total iron">486.11 (39.06) M</Attribute>     <Attribute attribute_name="metagenomic">1</Attribute>     <Attribute attribute_name="environmental-sample">1</Attribute>     <Attribute attribute_name="sample-type" harmonized_name="sample_type" display_name="sample type">metagenomic assembly</Attribute>     <Attribute attribute_name="metagenome-source">aquifer metagenome</Attribute>     <Attribute attribute_name="derived-from" harmonized_name="derived_from" display_name="derived from">This BioSample is a metagenomic assembly obtained from the aquifer metagenome BioSample: SAMN14680028</Attribute>     <Attribute attribute_name="isolate" harmonized_name="isolate" display_name="isolate">GA_180221_E-1-1-MAG-43_4</Attribute>   </Attributes>   <Links>     <Link type="entrez" target="bioproject" label="PRJNA627655">627655</Link>   </Links>   <Status status="live" when="2021-11-02T18:40:26.907"/> </BioSample> </BioSampleSet>
