[2023-06-29 13:19:21,088] [INFO] DFAST_QC pipeline started.
[2023-06-29 13:19:21,090] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 13:19:21,090] [INFO] DQC Reference Directory: /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference
[2023-06-29 13:19:22,294] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 13:19:22,295] [INFO] Task started: Prodigal
[2023-06-29 13:19:22,295] [INFO] Running command: gunzip -c /var/lib/cwl/stgc1f7ebe1-334b-41ce-a6d8-8a360448a0b5/GCA_021443845.1_ASM2144384v1_genomic.fna.gz | prodigal -d GCA_021443845.1_ASM2144384v1_genomic.fna/cds.fna -a GCA_021443845.1_ASM2144384v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 13:19:28,105] [INFO] Task succeeded: Prodigal
[2023-06-29 13:19:28,106] [INFO] Task started: HMMsearch
[2023-06-29 13:19:28,106] [INFO] Running command: hmmsearch --tblout GCA_021443845.1_ASM2144384v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/reference_markers.hmm GCA_021443845.1_ASM2144384v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 13:19:28,338] [INFO] Task succeeded: HMMsearch
[2023-06-29 13:19:28,339] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgc1f7ebe1-334b-41ce-a6d8-8a360448a0b5/GCA_021443845.1_ASM2144384v1_genomic.fna.gz]
[2023-06-29 13:19:28,368] [INFO] Query marker FASTA was written to GCA_021443845.1_ASM2144384v1_genomic.fna/markers.fasta
[2023-06-29 13:19:28,369] [INFO] Task started: Blastn
[2023-06-29 13:19:28,369] [INFO] Running command: blastn -query GCA_021443845.1_ASM2144384v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/reference_markers.fasta -out GCA_021443845.1_ASM2144384v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 13:19:28,974] [INFO] Task succeeded: Blastn
[2023-06-29 13:19:28,978] [INFO] Selected 26 target genomes.
[2023-06-29 13:19:28,978] [INFO] Target genome list was writen to GCA_021443845.1_ASM2144384v1_genomic.fna/target_genomes.txt
[2023-06-29 13:19:28,982] [INFO] Task started: fastANI
[2023-06-29 13:19:28,982] [INFO] Running command: fastANI --query /var/lib/cwl/stgc1f7ebe1-334b-41ce-a6d8-8a360448a0b5/GCA_021443845.1_ASM2144384v1_genomic.fna.gz --refList GCA_021443845.1_ASM2144384v1_genomic.fna/target_genomes.txt --output GCA_021443845.1_ASM2144384v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 13:19:42,789] [INFO] Task succeeded: fastANI
[2023-06-29 13:19:42,790] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 13:19:42,790] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 13:19:42,796] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 13:19:42,797] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 13:19:42,797] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 13:19:42,801] [INFO] DFAST Taxonomy check result was written to GCA_021443845.1_ASM2144384v1_genomic.fna/tc_result.tsv
[2023-06-29 13:19:42,802] [INFO] ===== Taxonomy check completed =====
[2023-06-29 13:19:42,802] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 13:19:42,803] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/checkm_data
[2023-06-29 13:19:42,808] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 13:19:42,841] [INFO] Task started: CheckM
[2023-06-29 13:19:42,841] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_021443845.1_ASM2144384v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_021443845.1_ASM2144384v1_genomic.fna/checkm_input GCA_021443845.1_ASM2144384v1_genomic.fna/checkm_result
[2023-06-29 13:20:07,352] [INFO] Task succeeded: CheckM
[2023-06-29 13:20:07,354] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 68.98%
Contamintation: 0.46%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-29 13:20:07,378] [INFO] ===== Completeness check finished =====
[2023-06-29 13:20:07,379] [INFO] ===== Start GTDB Search =====
[2023-06-29 13:20:07,379] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_021443845.1_ASM2144384v1_genomic.fna/markers.fasta)
[2023-06-29 13:20:07,380] [INFO] Task started: Blastn
[2023-06-29 13:20:07,380] [INFO] Running command: blastn -query GCA_021443845.1_ASM2144384v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg649b8bd7-64bb-4a94-8972-53b980a3085c/dqc_reference/reference_markers_gtdb.fasta -out GCA_021443845.1_ASM2144384v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 13:20:08,327] [INFO] Task succeeded: Blastn
[2023-06-29 13:20:08,331] [INFO] Selected 28 target genomes.
[2023-06-29 13:20:08,332] [INFO] Target genome list was writen to GCA_021443845.1_ASM2144384v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 13:20:08,351] [INFO] Task started: fastANI
[2023-06-29 13:20:08,352] [INFO] Running command: fastANI --query /var/lib/cwl/stgc1f7ebe1-334b-41ce-a6d8-8a360448a0b5/GCA_021443845.1_ASM2144384v1_genomic.fna.gz --refList GCA_021443845.1_ASM2144384v1_genomic.fna/target_genomes_gtdb.txt --output GCA_021443845.1_ASM2144384v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 13:20:20,332] [INFO] Task succeeded: fastANI
[2023-06-29 13:20:20,337] [INFO] Found 3 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 13:20:20,337] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_017510065.1	s__RGIG5612 sp017510065	76.7157	85	724	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__RGIG5612	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017431485.1	s__RGIG5612 sp017431485	76.4137	63	724	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__RGIG5612	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017459985.1	s__RGIG5612 sp017459985	76.3528	91	724	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__RGIG5612	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-29 13:20:20,339] [INFO] GTDB search result was written to GCA_021443845.1_ASM2144384v1_genomic.fna/result_gtdb.tsv
[2023-06-29 13:20:20,340] [INFO] ===== GTDB Search completed =====
[2023-06-29 13:20:20,342] [INFO] DFAST_QC result json was written to GCA_021443845.1_ASM2144384v1_genomic.fna/dqc_result.json
[2023-06-29 13:20:20,342] [INFO] DFAST_QC completed!
[2023-06-29 13:20:20,342] [INFO] Total running time: 0h0m59s