[2023-06-29 14:37:38,808] [INFO] DFAST_QC pipeline started.
[2023-06-29 14:37:38,811] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 14:37:38,811] [INFO] DQC Reference Directory: /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference
[2023-06-29 14:37:40,006] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 14:37:40,007] [INFO] Task started: Prodigal
[2023-06-29 14:37:40,007] [INFO] Running command: gunzip -c /var/lib/cwl/stg9c73db19-f225-4077-a157-c2e0d004e7d8/GCA_021621005.1_ASM2162100v1_genomic.fna.gz | prodigal -d GCA_021621005.1_ASM2162100v1_genomic.fna/cds.fna -a GCA_021621005.1_ASM2162100v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 14:37:43,411] [INFO] Task succeeded: Prodigal
[2023-06-29 14:37:43,411] [INFO] Task started: HMMsearch
[2023-06-29 14:37:43,411] [INFO] Running command: hmmsearch --tblout GCA_021621005.1_ASM2162100v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/reference_markers.hmm GCA_021621005.1_ASM2162100v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 14:37:43,641] [INFO] Task succeeded: HMMsearch
[2023-06-29 14:37:43,642] [INFO] Found 6/6 markers.
[2023-06-29 14:37:43,665] [INFO] Query marker FASTA was written to GCA_021621005.1_ASM2162100v1_genomic.fna/markers.fasta
[2023-06-29 14:37:43,666] [INFO] Task started: Blastn
[2023-06-29 14:37:43,666] [INFO] Running command: blastn -query GCA_021621005.1_ASM2162100v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/reference_markers.fasta -out GCA_021621005.1_ASM2162100v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 14:37:44,289] [INFO] Task succeeded: Blastn
[2023-06-29 14:37:44,294] [INFO] Selected 15 target genomes.
[2023-06-29 14:37:44,294] [INFO] Target genome list was writen to GCA_021621005.1_ASM2162100v1_genomic.fna/target_genomes.txt
[2023-06-29 14:37:44,299] [INFO] Task started: fastANI
[2023-06-29 14:37:44,299] [INFO] Running command: fastANI --query /var/lib/cwl/stg9c73db19-f225-4077-a157-c2e0d004e7d8/GCA_021621005.1_ASM2162100v1_genomic.fna.gz --refList GCA_021621005.1_ASM2162100v1_genomic.fna/target_genomes.txt --output GCA_021621005.1_ASM2162100v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 14:37:52,952] [INFO] Task succeeded: fastANI
[2023-06-29 14:37:52,953] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 14:37:52,953] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 14:37:52,956] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 14:37:52,956] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 14:37:52,956] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 14:37:52,959] [INFO] DFAST Taxonomy check result was written to GCA_021621005.1_ASM2162100v1_genomic.fna/tc_result.tsv
[2023-06-29 14:37:52,959] [INFO] ===== Taxonomy check completed =====
[2023-06-29 14:37:52,960] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 14:37:52,960] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/checkm_data
[2023-06-29 14:37:52,965] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 14:37:52,989] [INFO] Task started: CheckM
[2023-06-29 14:37:52,990] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_021621005.1_ASM2162100v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_021621005.1_ASM2162100v1_genomic.fna/checkm_input GCA_021621005.1_ASM2162100v1_genomic.fna/checkm_result
[2023-06-29 14:38:10,787] [INFO] Task succeeded: CheckM
[2023-06-29 14:38:10,788] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 14:38:10,808] [INFO] ===== Completeness check finished =====
[2023-06-29 14:38:10,808] [INFO] ===== Start GTDB Search =====
[2023-06-29 14:38:10,809] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_021621005.1_ASM2162100v1_genomic.fna/markers.fasta)
[2023-06-29 14:38:10,809] [INFO] Task started: Blastn
[2023-06-29 14:38:10,809] [INFO] Running command: blastn -query GCA_021621005.1_ASM2162100v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga6692a20-609e-427e-b458-533a03d812a5/dqc_reference/reference_markers_gtdb.fasta -out GCA_021621005.1_ASM2162100v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 14:38:11,838] [INFO] Task succeeded: Blastn
[2023-06-29 14:38:11,843] [INFO] Selected 19 target genomes.
[2023-06-29 14:38:11,843] [INFO] Target genome list was writen to GCA_021621005.1_ASM2162100v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 14:38:11,864] [INFO] Task started: fastANI
[2023-06-29 14:38:11,864] [INFO] Running command: fastANI --query /var/lib/cwl/stg9c73db19-f225-4077-a157-c2e0d004e7d8/GCA_021621005.1_ASM2162100v1_genomic.fna.gz --refList GCA_021621005.1_ASM2162100v1_genomic.fna/target_genomes_gtdb.txt --output GCA_021621005.1_ASM2162100v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 14:38:18,301] [INFO] Task succeeded: fastANI
[2023-06-29 14:38:18,318] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 14:38:18,319] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_000432435.1	s__Fimenecus sp000432435	98.4765	447	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	98.55	98.08	0.94	0.85	30	conclusive
GCA_002437245.1	s__Fimenecus sp002437245	89.7149	420	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_004556705.1	s__Fimenecus sp004556705	78.6437	108	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	96.73	95.72	0.84	0.76	9	-
GCA_900315985.1	s__Fimenecus sp900315985	77.5617	75	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	97.95	96.57	0.90	0.84	11	-
GCA_015066635.1	s__Fimenecus sp015066635	77.4648	61	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018712805.1	s__Fimenecus excrementavium	77.2085	52	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	98.06	98.06	0.85	0.85	2	-
GCA_017438845.1	s__Fimenecus sp017438845	77.1068	82	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015066565.1	s__SIG554 sp015066565	77.077	61	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__SIG554	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900540295.1	s__Fimenecus sp900540295	76.6223	60	472	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Fimenecus	95.0	98.52	98.30	0.90	0.86	3	-
--------------------------------------------------------------------------------
[2023-06-29 14:38:18,321] [INFO] GTDB search result was written to GCA_021621005.1_ASM2162100v1_genomic.fna/result_gtdb.tsv
[2023-06-29 14:38:18,322] [INFO] ===== GTDB Search completed =====
[2023-06-29 14:38:18,325] [INFO] DFAST_QC result json was written to GCA_021621005.1_ASM2162100v1_genomic.fna/dqc_result.json
[2023-06-29 14:38:18,325] [INFO] DFAST_QC completed!
[2023-06-29 14:38:18,326] [INFO] Total running time: 0h0m40s